1obb

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{{Seed}}
 
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[[Image:1obb.png|left|200px]]
 
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==alpha-glucosidase A, AglA, from Thermotoga maritima in complex with maltose and NAD+==
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The line below this paragraph, containing "STRUCTURE_1obb", creates the "Structure Box" on the page.
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<StructureSection load='1obb' size='340' side='right'caption='[[1obb]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1obb]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermotoga_maritima_MSB8 Thermotoga maritima MSB8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OBB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1OBB FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CSD:3-SULFINOALANINE'>CSD</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene>, <scene name='pdbligand=PRD_900001:alpha-maltose'>PRD_900001</scene></td></tr>
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{{STRUCTURE_1obb| PDB=1obb | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1obb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1obb OCA], [https://pdbe.org/1obb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1obb RCSB], [https://www.ebi.ac.uk/pdbsum/1obb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1obb ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/AGLA_THEMA AGLA_THEMA] alpha-glycosidase with a very broad specificity hydrolyzes maltose and other small maltooligosaccharides but is inactive against the polymeric substrate starch agla is not specific with respect to the configuration at the c-4 position of its substrates because glycosidic derivatives of d-galactose are also hydrolyzed does not cleave beta-glycosidic bonds
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ob/1obb_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1obb ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Glycoside hydrolase family 4 represents an unusual group of glucosidases with a requirement for NAD+, divalent metal cations, and reducing conditions. The family is also unique in its inclusion of both alpha- and beta-specific enzymes. The alpha-glucosidase A, AglA, from Thermotoga maritima is a typical glycoside hydrolase family 4 enzyme, requiring NAD+ and Mn2+ as well as strongly reducing conditions for activity. Here we present the crystal structure of the protein complexed with NAD+ and maltose, refined at a resolution of 1.9 A. The NAD+ is bound to a typical Rossman fold NAD+-binding site, and the nicotinamide moiety is localized close to the maltose substrate. Within the active site the conserved Cys-174 and surrounding histidines are positioned to play a role in the hydrolysis reaction. The electron density maps indicate that Cys-174 is oxidized to a sulfinic acid. Most likely, the strongly reducing conditions are necessary to reduce the oxidized cysteine side chain. Notably, the canonical set of catalytic acidic residues common to other glucosidases is not present in the active site. This, combined with a high structural homology to NAD-dependent dehydrogenases, suggests an unusual and possibly unique mechanism of action for a glycoside-hydrolyzing enzyme.
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===ALPHA-GLUCOSIDASE A, AGLA, FROM THERMOTOGA MARITIMA IN COMPLEX WITH MALTOSE AND NAD+===
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Crystal structure of Thermotoga maritima alpha-glucosidase AglA defines a new clan of NAD+-dependent glycosidases.,Lodge JA, Maier T, Liebl W, Hoffmann V, Strater N J Biol Chem. 2003 May 23;278(21):19151-8. Epub 2003 Feb 14. PMID:12588867<ref>PMID:12588867</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 1obb" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_12588867}}, adds the Publication Abstract to the page
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*[[Alpha-glucosidase 3D structures|Alpha-glucosidase 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 12588867 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_12588867}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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1OBB is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Thermotoga_maritima Thermotoga maritima]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OBB OCA].
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[[Category: Thermotoga maritima MSB8]]
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[[Category: Hoffmann V]]
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==Reference==
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[[Category: Liebl W]]
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Crystal structure of Thermotoga maritima alpha-glucosidase AglA defines a new clan of NAD+-dependent glycosidases., Lodge JA, Maier T, Liebl W, Hoffmann V, Strater N, J Biol Chem. 2003 May 23;278(21):19151-8. Epub 2003 Feb 14. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12588867 12588867]
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[[Category: Lodge JA]]
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[[Category: Alpha-glucosidase]]
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[[Category: Maier T]]
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[[Category: Single protein]]
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[[Category: Strater N]]
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[[Category: Thermotoga maritima]]
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[[Category: Hoffmann, V.]]
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[[Category: Liebl, W.]]
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[[Category: Lodge, J A.]]
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[[Category: Maier, T.]]
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[[Category: Strater, N.]]
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[[Category: Glycosidase]]
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[[Category: Hydrolase]]
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[[Category: Maltose]]
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[[Category: Nad+]]
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[[Category: Sulfinic acid]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 14:05:37 2008''
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Current revision

alpha-glucosidase A, AglA, from Thermotoga maritima in complex with maltose and NAD+

PDB ID 1obb

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