1jy2

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(New page: 200px<br /><applet load="1jy2" size="450" color="white" frame="true" align="right" spinBox="true" caption="1jy2, resolution 1.4&Aring;" /> '''Crystal Structure of ...)
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[[Image:1jy2.jpg|left|200px]]<br /><applet load="1jy2" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1jy2, resolution 1.4&Aring;" />
 
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'''Crystal Structure of the Central Region of Bovine Fibrinogen (E5 fragment) at 1.4 Angstroms Resolution'''<br />
 
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==Overview==
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==Crystal Structure of the Central Region of Bovine Fibrinogen (E5 fragment) at 1.4 Angstroms Resolution==
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The high-resolution crystal structure of the N-terminal central region of, bovine fibrinogen (a 35-kDa E(5) fragment) reveals a remarkable dimeric, design. The two halves of the molecule bond together at the center in an, extensive molecular "handshake" by using both disulfide linkages and, noncovalent contacts. On one face of the fragment, the Aalpha and Bbeta, chains from the two monomers form a funnel-shaped domain with an unusual, hydrophobic cavity; here, on each of the two outer sides there appears to, be a binding site for thrombin. On the opposite face, the N-terminal gamma, chains fold into a separate domain. Despite the chemical identity of the, two halves of fibrinogen, an unusual pair of adjacent disulfide bonds, locally constrain the two gamma chains to adopt different conformations., The striking asymmetry of this domain may promote the known supercoiling, of the protofibrils in fibrin. This information on the detailed topology, of the E(5) fragment permits the construction of a more detailed model, than previously possible for the critical trimolecular junction of the, protofibril in fibrin.
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<StructureSection load='1jy2' size='340' side='right'caption='[[1jy2]], [[Resolution|resolution]] 1.40&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1jy2]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JY2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1JY2 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.4&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1jy2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jy2 OCA], [https://pdbe.org/1jy2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1jy2 RCSB], [https://www.ebi.ac.uk/pdbsum/1jy2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1jy2 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/FIBA_BOVIN FIBA_BOVIN] Fibrinogen has a double function: yielding monomers that polymerize into fibrin and acting as a cofactor in platelet aggregation.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/jy/1jy2_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1jy2 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The high-resolution crystal structure of the N-terminal central region of bovine fibrinogen (a 35-kDa E(5) fragment) reveals a remarkable dimeric design. The two halves of the molecule bond together at the center in an extensive molecular "handshake" by using both disulfide linkages and noncovalent contacts. On one face of the fragment, the Aalpha and Bbeta chains from the two monomers form a funnel-shaped domain with an unusual hydrophobic cavity; here, on each of the two outer sides there appears to be a binding site for thrombin. On the opposite face, the N-terminal gamma chains fold into a separate domain. Despite the chemical identity of the two halves of fibrinogen, an unusual pair of adjacent disulfide bonds locally constrain the two gamma chains to adopt different conformations. The striking asymmetry of this domain may promote the known supercoiling of the protofibrils in fibrin. This information on the detailed topology of the E(5) fragment permits the construction of a more detailed model than previously possible for the critical trimolecular junction of the protofibril in fibrin.
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==About this Structure==
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Crystal structure of the central region of bovine fibrinogen (E5 fragment) at 1.4-A resolution.,Madrazo J, Brown JH, Litvinovich S, Dominguez R, Yakovlev S, Medved L, Cohen C Proc Natl Acad Sci U S A. 2001 Oct 9;98(21):11967-72. PMID:11593005<ref>PMID:11593005</ref>
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1JY2 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1JY2 OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Crystal structure of the central region of bovine fibrinogen (E5 fragment) at 1.4-A resolution., Madrazo J, Brown JH, Litvinovich S, Dominguez R, Yakovlev S, Medved L, Cohen C, Proc Natl Acad Sci U S A. 2001 Oct 9;98(21):11967-72. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11593005 11593005]
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</div>
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[[Category: Bos taurus]]
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<div class="pdbe-citations 1jy2" style="background-color:#fffaf0;"></div>
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[[Category: Protein complex]]
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[[Category: Brown, J.H.]]
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[[Category: Cohen, C.]]
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[[Category: Dominguez, R.]]
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[[Category: Litvinovich, S.]]
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[[Category: Madrazo, J.]]
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[[Category: Medved, L.]]
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[[Category: Yakovlev, S.]]
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[[Category: asymmetry]]
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[[Category: beta-sheet]]
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[[Category: coiled-coil]]
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[[Category: crystal structure]]
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[[Category: disulfide bonds]]
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[[Category: fibrinogen]]
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[[Category: fragment e]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 18:40:52 2007''
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==See Also==
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*[[Fibrinogen|Fibrinogen]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Bos taurus]]
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[[Category: Large Structures]]
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[[Category: Brown JH]]
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[[Category: Cohen C]]
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[[Category: Dominguez R]]
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[[Category: Litvinovich S]]
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[[Category: Madrazo J]]
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[[Category: Medved L]]
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[[Category: Yakovlev S]]

Current revision

Crystal Structure of the Central Region of Bovine Fibrinogen (E5 fragment) at 1.4 Angstroms Resolution

PDB ID 1jy2

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