2h34

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{{Seed}}
 
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[[Image:2h34.png|left|200px]]
 
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==Apoenzyme crystal structure of the tuberculosis serine/threonine kinase, PknE==
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The line below this paragraph, containing "STRUCTURE_2h34", creates the "Structure Box" on the page.
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<StructureSection load='2h34' size='340' side='right'caption='[[2h34]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2h34]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_H37Rv Mycobacterium tuberculosis H37Rv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2H34 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2H34 FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BR:BROMIDE+ION'>BR</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
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{{STRUCTURE_2h34| PDB=2h34 | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2h34 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2h34 OCA], [https://pdbe.org/2h34 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2h34 RCSB], [https://www.ebi.ac.uk/pdbsum/2h34 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2h34 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/PKNE_MYCTU PKNE_MYCTU] Important for survival of the bacterium in the host during infection. Promotes the survival of infected macrophages by activating multiple signaling responses and suppressing apoptosis of macrophages during nitrate stress. May contribute to the adaptation of M.tuberculosis during stress conditions by maintaining the cellular integrity. Can phosphorylate the FHA domain of Rv1747.<ref>PMID:15987910</ref> <ref>PMID:17892498</ref> <ref>PMID:23108860</ref> <ref>PMID:21945589</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/h3/2h34_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2h34 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The "eukaryotic-like" receptor Ser/Thr protein kinases (STPKs) are candidates for the sensors that mediate environmental adaptations of Mycobacterium tuberculosis (Mtb). To define the mechanisms of regulation and substrate recognition, we determined the crystal structure of the ligand-free, activated kinase domain (KD) of the Mtb STPK, PknE. Remarkably, the PknE KD formed a dimer similar to that first observed in the structure of the ATPgammaS complex of the Mtb paralog, PknB. This structural similarity, which occurs despite little sequence conservation between the PknB and PknE dimer interfaces, supports the idea that dimerization regulates the Mtb receptor STPKs. Insertion of the DFG motif into the ATP-binding site and other conformational differences compared the ATPgammaS:PknB complex suggest that apo-PknE is not pre-organized to bind nucleotides. This structure may represent an inactive conformation stabilized by dimerization or, alternatively, an active conformation that reveals shifts that mediate nucleotide exchange and order substrate binding.
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===Apoenzyme crystal structure of the tuberculosis serine/threonine kinase, PknE===
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A conserved dimer and global conformational changes in the structure of apo-PknE Ser/Thr protein kinase from Mycobacterium tuberculosis.,Gay LM, Ng HL, Alber T J Mol Biol. 2006 Jul 7;360(2):409-20. Epub 2006 May 19. PMID:16762364<ref>PMID:16762364</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 2h34" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_16762364}}, adds the Publication Abstract to the page
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*[[Serine/threonine protein kinase 3D structures|Serine/threonine protein kinase 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 16762364 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_16762364}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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2H34 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2H34 OCA].
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[[Category: Mycobacterium tuberculosis H37Rv]]
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[[Category: Alber T]]
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==Reference==
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[[Category: Gay LM]]
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A conserved dimer and global conformational changes in the structure of apo-PknE Ser/Thr protein kinase from Mycobacterium tuberculosis., Gay LM, Ng HL, Alber T, J Mol Biol. 2006 Jul 7;360(2):409-20. Epub 2006 May 19. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16762364 16762364]
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[[Category: Ng HL]]
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[[Category: Mycobacterium tuberculosis]]
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[[Category: Non-specific serine/threonine protein kinase]]
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[[Category: Single protein]]
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[[Category: Alber, T.]]
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[[Category: Gay, L M.]]
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[[Category: Ng, H L.]]
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[[Category: Apoenzyme]]
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[[Category: Serine/threonine protein kinase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 14:15:05 2008''
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Current revision

Apoenzyme crystal structure of the tuberculosis serine/threonine kinase, PknE

PDB ID 2h34

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