1jzo

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(New page: 200px<br /><applet load="1jzo" size="450" color="white" frame="true" align="right" spinBox="true" caption="1jzo, resolution 1.92&Aring;" /> '''DsbC C101S'''<br /> ...)
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[[Image:1jzo.gif|left|200px]]<br /><applet load="1jzo" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1jzo, resolution 1.92&Aring;" />
 
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'''DsbC C101S'''<br />
 
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==Overview==
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==DsbC C101S==
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The Escherichia coli disulfide bond isomerase DsbC rearranges incorrect, disulfide bonds during oxidative protein folding. It is specifically, activated by the periplasmic N-terminal domain (DsbDalpha) of the, transmembrane electron transporter DsbD. An intermediate of the electron, transport reaction was trapped, yielding a covalent DsbC-DsbDalpha, complex. The 2.3 A crystal structure of the complex shows for the first, time the specific interactions between two thiol oxidoreductases., DsbDalpha is a novel thiol oxidoreductase with the active site cysteines, embedded in an immunoglobulin fold. It binds into the central cleft of the, V-shaped DsbC dimer, which assumes a closed conformation on complex, formation. Comparison of the complex with oxidized DsbDalpha reveals major, conformational changes in a cap structure that regulates the accessibility, of the DsbDalpha active site. Our results explain how DsbC is selectively, activated by DsbD using electrons derived from the cytoplasm.
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<StructureSection load='1jzo' size='340' side='right'caption='[[1jzo]], [[Resolution|resolution]] 1.92&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1jzo]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JZO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1JZO FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.92&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1jzo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jzo OCA], [https://pdbe.org/1jzo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1jzo RCSB], [https://www.ebi.ac.uk/pdbsum/1jzo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1jzo ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/DSBC_ECOLI DSBC_ECOLI] Acts as a disulfide isomerase, interacting with incorrectly folded proteins to correct non-native disulfide bonds. DsbG and DsbC are part of a periplasmic reducing system that controls the level of cysteine sulfenylation, and provides reducing equivalents to rescue oxidatively damaged secreted proteins. Acts by transferring its disulfide bond to other proteins and is reduced in the process. DsbC is reoxidized by DsbD.<ref>PMID:19965429</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/jz/1jzo_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1jzo ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The Escherichia coli disulfide bond isomerase DsbC rearranges incorrect disulfide bonds during oxidative protein folding. It is specifically activated by the periplasmic N-terminal domain (DsbDalpha) of the transmembrane electron transporter DsbD. An intermediate of the electron transport reaction was trapped, yielding a covalent DsbC-DsbDalpha complex. The 2.3 A crystal structure of the complex shows for the first time the specific interactions between two thiol oxidoreductases. DsbDalpha is a novel thiol oxidoreductase with the active site cysteines embedded in an immunoglobulin fold. It binds into the central cleft of the V-shaped DsbC dimer, which assumes a closed conformation on complex formation. Comparison of the complex with oxidized DsbDalpha reveals major conformational changes in a cap structure that regulates the accessibility of the DsbDalpha active site. Our results explain how DsbC is selectively activated by DsbD using electrons derived from the cytoplasm.
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==About this Structure==
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The disulfide bond isomerase DsbC is activated by an immunoglobulin-fold thiol oxidoreductase: crystal structure of the DsbC-DsbDalpha complex.,Haebel PW, Goldstone D, Katzen F, Beckwith J, Metcalf P EMBO J. 2002 Sep 16;21(18):4774-84. PMID:12234918<ref>PMID:12234918</ref>
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1JZO is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1JZO OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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The disulfide bond isomerase DsbC is activated by an immunoglobulin-fold thiol oxidoreductase: crystal structure of the DsbC-DsbDalpha complex., Haebel PW, Goldstone D, Katzen F, Beckwith J, Metcalf P, EMBO J. 2002 Sep 16;21(18):4774-84. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12234918 12234918]
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</div>
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[[Category: Escherichia coli]]
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<div class="pdbe-citations 1jzo" style="background-color:#fffaf0;"></div>
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[[Category: Single protein]]
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[[Category: Beckwith, J.]]
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[[Category: Goldstone, D.]]
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[[Category: Haebel, P.W.]]
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[[Category: Katzen, F.]]
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[[Category: Metcalf, P.]]
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[[Category: disulfide bond isomerase]]
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[[Category: dsbc]]
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[[Category: thiol oxidoreductase]]
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[[Category: thioredoxin fold]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 18:43:29 2007''
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==See Also==
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*[[Thiol:disulfide interchange protein 3D structures|Thiol:disulfide interchange protein 3D structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Escherichia coli]]
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[[Category: Large Structures]]
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[[Category: Beckwith J]]
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[[Category: Goldstone D]]
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[[Category: Haebel PW]]
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[[Category: Katzen F]]
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[[Category: Metcalf P]]

Current revision

DsbC C101S

PDB ID 1jzo

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