1jzq

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(New page: 200px<br /><applet load="1jzq" size="450" color="white" frame="true" align="right" spinBox="true" caption="1jzq, resolution 3.0&Aring;" /> '''Isoleucyl-tRNA synthe...)
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[[Image:1jzq.gif|left|200px]]<br /><applet load="1jzq" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1jzq, resolution 3.0&Aring;" />
 
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'''Isoleucyl-tRNA synthetase Complexed with Isoleucyl-adenylate analogue'''<br />
 
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==Overview==
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==Isoleucyl-tRNA synthetase Complexed with Isoleucyl-adenylate analogue==
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An analogue of isoleucyl-adenylate (Ile-AMS) potently inhibits the, isoleucyl-tRNA synthetases (IleRSs) from the three primary kingdoms, whereas the antibiotic mupirocin inhibits only the eubacterial and, archaeal IleRSs, but not the eukaryotic enzymes, and therefore is, clinically used against methicillin-resistant Staphylococcus aureus. We, determined the crystal structures of the IleRS from the thermophilic, eubacterium, Thermus thermophilus, in complexes with Ile-AMS and mupirocin, at 3.0- and 2.5-A resolutions, respectively. A structural comparison of, the IleRS.Ile-AMS complex with the adenylate complexes of other, aminoacyl-tRNA synthetases revealed the common recognition mode of, aminoacyl-adenylate by the class I aminoacyl-tRNA synthetases. The Ile-AMS, and mupirocin, which have significantly different chemical structures, are, recognized by many of the same amino acid residues of the IleRS, suggesting that the antibiotic inhibits the enzymatic activity by blocking, the binding site of the high energy intermediate, Ile-AMP. In contrast, the two amino acid residues that concomitantly recognize Ile-AMS and, mupirocin are different between the eubacterial/archaeal IleRSs and the, eukaryotic IleRSs. Mutagenic analyses revealed that the replacement of the, two residues significantly changed the sensitivity to mupirocin.
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<StructureSection load='1jzq' size='340' side='right'caption='[[1jzq]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1jzq]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JZQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1JZQ FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ILA:N-[ISOLEUCINYL]-N-[ADENOSYL]-DIAMINOSUFONE'>ILA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1jzq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jzq OCA], [https://pdbe.org/1jzq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1jzq RCSB], [https://www.ebi.ac.uk/pdbsum/1jzq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1jzq ProSAT], [https://www.topsan.org/Proteins/RSGI/1jzq TOPSAN]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/SYI_THET8 SYI_THET8] Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) (By similarity).[HAMAP-Rule:MF_02003]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/jz/1jzq_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1jzq ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1JZQ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus] with ZN and ILA as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Isoleucine--tRNA_ligase Isoleucine--tRNA ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.1.1.5 6.1.1.5] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1JZQ OCA].
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*[[Aminoacyl tRNA synthetase 3D structures|Aminoacyl tRNA synthetase 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Structural basis for the recognition of isoleucyl-adenylate and an antibiotic, mupirocin, by isoleucyl-tRNA synthetase., Nakama T, Nureki O, Yokoyama S, J Biol Chem. 2001 Dec 14;276(50):47387-93. Epub 2001 Oct 2. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11584022 11584022]
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[[Category: Large Structures]]
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[[Category: Isoleucine--tRNA ligase]]
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[[Category: Single protein]]
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[[Category: Thermus thermophilus]]
[[Category: Thermus thermophilus]]
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[[Category: Nakama, T.]]
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[[Category: Nakama T]]
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[[Category: Nureki, O.]]
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[[Category: Nureki O]]
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[[Category: RSGI, RIKEN.Structural.Genomics/Proteomics.Initiative.]]
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[[Category: Yokoyama S]]
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[[Category: Yokoyama, S.]]
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[[Category: ILA]]
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[[Category: ZN]]
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[[Category: aminoacyl-trna synthetase]]
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[[Category: riken structural genomics/proteomics initiative]]
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[[Category: rsgi]]
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[[Category: structural genomics]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 18:43:33 2007''
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Current revision

Isoleucyl-tRNA synthetase Complexed with Isoleucyl-adenylate analogue

PDB ID 1jzq

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