2bke

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (07:34, 9 October 2024) (edit) (undo)
 
(11 intermediate revisions not shown.)
Line 1: Line 1:
-
{{Seed}}
 
-
[[Image:2bke.png|left|200px]]
 
-
<!--
+
==Conformational Flexibility Revealed by the Crystal Structure of a Crenarchaeal RadA==
-
The line below this paragraph, containing "STRUCTURE_2bke", creates the "Structure Box" on the page.
+
<StructureSection load='2bke' size='340' side='right'caption='[[2bke]], [[Resolution|resolution]] 3.20&Aring;' scene=''>
-
You may change the PDB parameter (which sets the PDB file loaded into the applet)
+
== Structural highlights ==
-
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+
<table><tr><td colspan='2'>[[2bke]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharolobus_solfataricus_P2 Saccharolobus solfataricus P2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BKE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2BKE FirstGlance]. <br>
-
or leave the SCENE parameter empty for the default display.
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.2&#8491;</td></tr>
-
-->
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
-
{{STRUCTURE_2bke| PDB=2bke | SCENE= }}
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2bke FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bke OCA], [https://pdbe.org/2bke PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2bke RCSB], [https://www.ebi.ac.uk/pdbsum/2bke PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2bke ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/RADA_SACS2 RADA_SACS2] Involved in DNA repair and in homologous recombination. Binds and assemble on single-stranded DNA to form a nucleoprotein filament. Hydrolyzes ATP in a ssDNA-dependent manner and promotes DNA strand exchange between homologous DNA molecules.
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bk/2bke_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2bke ConSurf].
 +
<div style="clear:both"></div>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
Homologous recombinational repair is an essential mechanism for repair of double-strand breaks in DNA. Recombinases of the RecA-fold family play a crucial role in this process, forming filaments that utilize ATP to mediate their interactions with single- and double-stranded DNA. The recombinase molecules present in the archaea (RadA) and eukaryota (Rad51) are more closely related to each other than to their bacterial counterpart (RecA) and, as a result, RadA makes a suitable model for the eukaryotic system. The crystal structure of Sulfolobus solfataricus RadA has been solved to a resolution of 3.2 A in the absence of nucleotide analogues or DNA, revealing a narrow filamentous assembly with three molecules per helical turn. As observed in other RecA-family recombinases, each RadA molecule in the filament is linked to its neighbour via interactions of a short beta-strand with the neighbouring ATPase domain. However, despite apparent flexibility between domains, comparison with other structures indicates conservation of a number of key interactions that introduce rigidity to the system, allowing allosteric control of the filament by interaction with ATP. Additional analysis reveals that the interaction specificity of the five human Rad51 paralogues can be predicted using a simple model based on the RadA structure.
-
===CONFORMATIONAL FLEXIBILITY REVEALED BY THE CRYSTAL STRUCTURE OF A CRENARCHAEAL RADA===
+
Conformational flexibility revealed by the crystal structure of a crenarchaeal RadA.,Ariza A, Richard DJ, White MF, Bond CS Nucleic Acids Res. 2005 Mar 8;33(5):1465-73. Print 2005. PMID:15755748<ref>PMID:15755748</ref>
-
 
+
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-
<!--
+
</div>
-
The line below this paragraph, {{ABSTRACT_PUBMED_15755748}}, adds the Publication Abstract to the page
+
<div class="pdbe-citations 2bke" style="background-color:#fffaf0;"></div>
-
(as it appears on PubMed at http://www.pubmed.gov), where 15755748 is the PubMed ID number.
+
== References ==
-
-->
+
<references/>
-
{{ABSTRACT_PUBMED_15755748}}
+
__TOC__
-
 
+
</StructureSection>
-
==About this Structure==
+
[[Category: Large Structures]]
-
2BKE is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Sulfolobus_solfataricus Sulfolobus solfataricus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BKE OCA].
+
[[Category: Saccharolobus solfataricus P2]]
-
 
+
[[Category: Ariza A]]
-
==Reference==
+
[[Category: Bond CS]]
-
Conformational flexibility revealed by the crystal structure of a crenarchaeal RadA., Ariza A, Richard DJ, White MF, Bond CS, Nucleic Acids Res. 2005 Mar 8;33(5):1465-73. Print 2005. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15755748 15755748]
+
[[Category: Richard DL]]
-
[[Category: Single protein]]
+
[[Category: White MF]]
-
[[Category: Sulfolobus solfataricus]]
+
-
[[Category: Ariza, A.]]
+
-
[[Category: Bond, C S.]]
+
-
[[Category: Richard, D L.]]
+
-
[[Category: White, M F.]]
+
-
[[Category: Archaea]]
+
-
[[Category: Dna repair]]
+
-
[[Category: Dna-binding protei]]
+
-
[[Category: Filament]]
+
-
[[Category: Homologous recombination]]
+
-
[[Category: Rad51]]
+
-
[[Category: Rada]]
+
-
[[Category: Reca]]
+
-
[[Category: Sulfolobus solfataricus]]
+
-
 
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 14:37:51 2008''
+

Current revision

Conformational Flexibility Revealed by the Crystal Structure of a Crenarchaeal RadA

PDB ID 2bke

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2bke"
Personal tools