2a6q

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[[Image:2a6q.png|left|200px]]
 
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==Crystal structure of YefM-YoeB complex==
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The line below this paragraph, containing "STRUCTURE_2a6q", creates the "Structure Box" on the page.
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<StructureSection load='2a6q' size='340' side='right'caption='[[2a6q]], [[Resolution|resolution]] 2.05&Aring;' scene=''>
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2a6q]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A6Q OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2A6Q FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.05&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2a6q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2a6q OCA], [https://pdbe.org/2a6q PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2a6q RCSB], [https://www.ebi.ac.uk/pdbsum/2a6q PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2a6q ProSAT]</span></td></tr>
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{{STRUCTURE_2a6q| PDB=2a6q | SCENE= }}
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</table>
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== Function ==
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===Crystal structure of YefM-YoeB complex===
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[https://www.uniprot.org/uniprot/YOEB_ECOLI YOEB_ECOLI] Toxic component of a toxin-antitoxin (TA) module. Its mode of function is controversial; it has been proposed to be an mRNA interferase but also an inhibitor of translation initiation. When overproduced in wild-type cells, inhibits bacterial growth and translation by cleavage of mRNA molecules while it has a weak effect on colony forming ability. Overproduction of Lon protease specifically activates YoeB-dependent mRNA cleavage, leading to lethality. YefM binds to the promoter region of the yefM-yeoB operon to repress transcription, YeoB acts as a corepressor.<ref>PMID:14672926</ref> <ref>PMID:15009896</ref> <ref>PMID:17170003</ref> <ref>PMID:18854355</ref> <ref>PMID:19028895</ref> <ref>PMID:19124462</ref> <ref>PMID:19400780</ref> <ref>PMID:16109374</ref> Shown in vitro to be an mRNA interferase that requires translation for substrate cleavage; if the mRNA is mutated so as to not be translatable it is no longer cleaved. Cleavage only occurs within translated regions. Has RNase activity and preferentially cleaves at the 3'-end of purine ribonucleotides.<ref>PMID:14672926</ref> <ref>PMID:15009896</ref> <ref>PMID:17170003</ref> <ref>PMID:18854355</ref> <ref>PMID:19028895</ref> <ref>PMID:19124462</ref> <ref>PMID:19400780</ref> <ref>PMID:16109374</ref> Also shown in vitro to be a translation initiation blocker. Binds to the 70S ribosome and 50S ribosomal subunit; binding is inhibited by hygromycin A and tetracycline, both of which bind to the 30S subunit in the A site. Thus YoeB is located at the interface between 50S and 30S ribosomes and interacts with the A site where it cleaves mRNA, blocking translation initiation.<ref>PMID:14672926</ref> <ref>PMID:15009896</ref> <ref>PMID:17170003</ref> <ref>PMID:18854355</ref> <ref>PMID:19028895</ref> <ref>PMID:19124462</ref> <ref>PMID:19400780</ref> <ref>PMID:16109374</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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(as it appears on PubMed at http://www.pubmed.gov), where 16109374 is the PubMed ID number.
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/a6/2a6q_consurf.spt"</scriptWhenChecked>
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{{ABSTRACT_PUBMED_16109374}}
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</jmolCheckbox>
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==About this Structure==
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2a6q ConSurf].
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2A6Q is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A6Q OCA].
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== References ==
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==Reference==
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<references/>
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Conformational change in the catalytic site of the ribonuclease YoeB toxin by YefM antitoxin., Kamada K, Hanaoka F, Mol Cell. 2005 Aug 19;19(4):497-509. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16109374 16109374]
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__TOC__
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</StructureSection>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Protein complex]]
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[[Category: Large Structures]]
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[[Category: Hanaoka, F.]]
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[[Category: Hanaoka F]]
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[[Category: Kamada, K.]]
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[[Category: Kamada K]]
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[[Category: Addiction module]]
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[[Category: Antitoxin]]
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[[Category: Inhibitor]]
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[[Category: Rnase]]
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[[Category: Toxin]]
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[[Category: Yefm]]
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[[Category: Yoeb]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 14:39:28 2008''
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Current revision

Crystal structure of YefM-YoeB complex

PDB ID 2a6q

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