1k0w

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(New page: 200px<br /><applet load="1k0w" size="450" color="white" frame="true" align="right" spinBox="true" caption="1k0w, resolution 2.10&Aring;" /> '''CRYSTAL STRUCTURE OF...)
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[[Image:1k0w.jpg|left|200px]]<br /><applet load="1k0w" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1k0w, resolution 2.10&Aring;" />
 
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'''CRYSTAL STRUCTURE OF L-RIBULOSE-5-PHOSPHATE 4-EPIMERASE'''<br />
 
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==Overview==
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==CRYSTAL STRUCTURE OF L-RIBULOSE-5-PHOSPHATE 4-EPIMERASE==
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The structure of L-ribulose-5-phosphate 4-epimerase from E. coli has been, solved to 2.4 A resolution using X-ray diffraction data. The structure is, homo-tetrameric and displays C(4) symmetry. Each subunit has a single, domain comprised of a central beta-sheet flanked on either side by layers, of alpha-helices. The active site is identified by the position of the, catalytic zinc residue and is located at the interface between two, adjacent subunits. A remarkable feature of the structure is that it shows, a very close resemblance to that of L-fuculose-1-phosphate aldolase. This, is consistent with the notion that both enzymes belong to a superfamily of, epimerases/aldolases that catalyze carbon-carbon bond cleavage reactions, via a metal-stabilized enolate intermediate. Detailed inspection of the, epimerase structure, however, indicates that despite the close overall, structural similarity to class II aldolases, the enzyme has evolved, distinct active site features that promote its particular chemistry.
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<StructureSection load='1k0w' size='340' side='right'caption='[[1k0w]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1k0w]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K0W OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1K0W FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1k0w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1k0w OCA], [https://pdbe.org/1k0w PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1k0w RCSB], [https://www.ebi.ac.uk/pdbsum/1k0w PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1k0w ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/ARAD_ECOLI ARAD_ECOLI]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/k0/1k0w_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1k0w ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The structure of L-ribulose-5-phosphate 4-epimerase from E. coli has been solved to 2.4 A resolution using X-ray diffraction data. The structure is homo-tetrameric and displays C(4) symmetry. Each subunit has a single domain comprised of a central beta-sheet flanked on either side by layers of alpha-helices. The active site is identified by the position of the catalytic zinc residue and is located at the interface between two adjacent subunits. A remarkable feature of the structure is that it shows a very close resemblance to that of L-fuculose-1-phosphate aldolase. This is consistent with the notion that both enzymes belong to a superfamily of epimerases/aldolases that catalyze carbon-carbon bond cleavage reactions via a metal-stabilized enolate intermediate. Detailed inspection of the epimerase structure, however, indicates that despite the close overall structural similarity to class II aldolases, the enzyme has evolved distinct active site features that promote its particular chemistry.
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==About this Structure==
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The structure of L-ribulose-5-phosphate 4-epimerase: an aldolase-like platform for epimerization.,Luo Y, Samuel J, Mosimann SC, Lee JE, Tanner ME, Strynadka NC Biochemistry. 2001 Dec 11;40(49):14763-71. PMID:11732895<ref>PMID:11732895</ref>
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1K0W is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with ZN as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/L-ribulose-5-phosphate_4-epimerase L-ribulose-5-phosphate 4-epimerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.1.3.4 5.1.3.4] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1K0W OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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The structure of L-ribulose-5-phosphate 4-epimerase: an aldolase-like platform for epimerization., Luo Y, Samuel J, Mosimann SC, Lee JE, Tanner ME, Strynadka NC, Biochemistry. 2001 Dec 11;40(49):14763-71. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11732895 11732895]
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</div>
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<div class="pdbe-citations 1k0w" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: L-ribulose-5-phosphate 4-epimerase]]
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[[Category: Large Structures]]
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[[Category: Single protein]]
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[[Category: Lee JE]]
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[[Category: Lee, J.E.]]
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[[Category: Luo Y]]
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[[Category: Luo, Y.]]
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[[Category: Mosimann SC]]
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[[Category: Mosimann, S.C.]]
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[[Category: Samuel J]]
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[[Category: Samuel, J.]]
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[[Category: Strynadka NCJ]]
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[[Category: Strynadka, N.C.J.]]
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[[Category: ZN]]
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[[Category: aldolase]]
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[[Category: epimerase]]
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[[Category: ribulose]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 18:45:55 2007''
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Current revision

CRYSTAL STRUCTURE OF L-RIBULOSE-5-PHOSPHATE 4-EPIMERASE

PDB ID 1k0w

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