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1uns

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(New page: 200px<br /> <applet load="1uns" size="450" color="white" frame="true" align="right" spinBox="true" caption="1uns, resolution 2.0&Aring;" /> '''IDENTIFICATION OF A ...)
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[[Image:1uns.gif|left|200px]]<br />
 
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<applet load="1uns" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1uns, resolution 2.0&Aring;" />
 
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'''IDENTIFICATION OF A SECONDARY ZINC-BINDING SITE IN STAPHYLOCOCCAL ENTEROTOXIN C2: IMPLICATIONS FOR SUPERANTIGEN RECOGNITION'''<br />
 
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==Overview==
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==IDENTIFICATION OF A SECONDARY ZINC-BINDING SITE IN STAPHYLOCOCCAL ENTEROTOXIN C2: IMPLICATIONS FOR SUPERANTIGEN RECOGNITION==
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The previously determined crystal structure of the superantigen, staphylococcal enterotoxin C2 (SEC2) showed binding of a single zinc ion, located between the N- and C-terminal domains. Here we present the crystal, structure of SEC2 determined to 2.0 A resolution in the presence of, additional zinc. The structure revealed the presence of a secondary, zinc-binding site close to the major histocompatibility complex, (MHC)-binding site of the toxin and some 28 A away from the primary, zinc-binding site of the toxin found in previous studies. T cell, stimulation assays showed that varying the concentration of zinc ions, present affected the activity of the toxin and we observed that high zinc, concentrations considerably inhibited T cell responses. This indicates, that SEC2 may have multiple ... [[http://ispc.weizmann.ac.il/pmbin/getpm?14559915 (full description)]]
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<StructureSection load='1uns' size='340' side='right'caption='[[1uns]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1uns]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UNS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1UNS FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1uns FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1uns OCA], [https://pdbe.org/1uns PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1uns RCSB], [https://www.ebi.ac.uk/pdbsum/1uns PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1uns ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/ENTC2_STAAU ENTC2_STAAU] Staphylococcal enterotoxins cause the intoxication staphylococcal food poisoning syndrome. The illness characterized by high fever, hypotension, diarrhea, shock, and in some cases death.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/un/1uns_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1uns ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The previously determined crystal structure of the superantigen staphylococcal enterotoxin C2 (SEC2) showed binding of a single zinc ion located between the N- and C-terminal domains. Here we present the crystal structure of SEC2 determined to 2.0 A resolution in the presence of additional zinc. The structure revealed the presence of a secondary zinc-binding site close to the major histocompatibility complex (MHC)-binding site of the toxin and some 28 A away from the primary zinc-binding site of the toxin found in previous studies. T cell stimulation assays showed that varying the concentration of zinc ions present affected the activity of the toxin and we observed that high zinc concentrations considerably inhibited T cell responses. This indicates that SEC2 may have multiple modes of interaction with the immune system that are dependent on serum zinc levels. The potential role of the secondary zinc-binding site and that of the primary one in the formation of the TCR.SEC2.MHC complex are considered, and the possibility that zinc may regulate the activity of SEC2 as a toxin facilitating different T cell responses is discussed.
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==About this Structure==
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Identification of a secondary zinc-binding site in staphylococcal enterotoxin C2. Implications for superantigen recognition.,Papageorgiou AC, Baker MD, McLeod JD, Goda SK, Manzotti CN, Sansom DM, Tranter HS, Acharya KR J Biol Chem. 2004 Jan 9;279(2):1297-303. Epub 2003 Oct 14. PMID:14559915<ref>PMID:14559915</ref>
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1UNS is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]] with ZN as [[http://en.wikipedia.org/wiki/ligand ligand]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1UNS OCA]].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Identification of a secondary zinc-binding site in staphylococcal enterotoxin C2. Implications for superantigen recognition., Papageorgiou AC, Baker MD, McLeod JD, Goda SK, Manzotti CN, Sansom DM, Tranter HS, Acharya KR, J Biol Chem. 2004 Jan 9;279(2):1297-303. Epub 2003 Oct 14. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=14559915 14559915]
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</div>
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[[Category: Single protein]]
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<div class="pdbe-citations 1uns" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Staphylococcus aureus]]
[[Category: Staphylococcus aureus]]
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[[Category: Acharya, K.R.]]
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[[Category: Acharya KR]]
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[[Category: Baker, M.D.]]
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[[Category: Baker MD]]
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[[Category: Goda, S.]]
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[[Category: Goda S]]
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[[Category: Mcleod, J.D.]]
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[[Category: McLeod JD]]
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[[Category: Papageorgiou, A.C.]]
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[[Category: Papageorgiou AC]]
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[[Category: Sansom, D.M.]]
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[[Category: Sansom DM]]
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[[Category: Tranter, H.S.]]
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[[Category: Tranter HS]]
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[[Category: ZN]]
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[[Category: enterotoxin]]
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[[Category: superantigen]]
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[[Category: toxin]]
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[[Category: zinc-binding site]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Oct 29 20:11:25 2007''
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Current revision

IDENTIFICATION OF A SECONDARY ZINC-BINDING SITE IN STAPHYLOCOCCAL ENTEROTOXIN C2: IMPLICATIONS FOR SUPERANTIGEN RECOGNITION

PDB ID 1uns

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