1k3h

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(New page: 200px<br /><applet load="1k3h" size="450" color="white" frame="true" align="right" spinBox="true" caption="1k3h" /> '''NMR Solution Structure of Oxidized Cytochrom...)
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[[Image:1k3h.jpg|left|200px]]<br /><applet load="1k3h" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1k3h" />
 
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'''NMR Solution Structure of Oxidized Cytochrome c-553 from Bacillus pasteurii'''<br />
 
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==Overview==
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==NMR Solution Structure of Oxidized Cytochrome c-553 from Bacillus pasteurii==
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The solution structure of oxidized cytochrome c(553) (71 amino acid, residues) from the Gram-positive bacterium Bacillus pasteurii is here, reported and compared with the available crystal structure. The solution, structure is obtained from 1609 meaningful NOE data (22.7 per residue), 76, dihedral angles, and 59 pseudocontact shifts. The root mean square, deviations from the average structure are 0.25+/-0.07 and 0.59+/-0.13 A, for the backbone and all heavy atoms, respectively, and the quality, assessment of the structure is satisfactory. The solution structure, closely reproduces the fold observed in the crystal structure. The, backbone mobility was then investigated through amide (15)N relaxation, rate and (15)N-(1)H NOE measurements. The protein is rigid in both the, sub-nanosecond and millisecond time scales, probably due to the relatively, large heme:number of amino acids ratio. Modeling of eight c-type, cytochromes from other Gram-positive bacteria with a high sequence, identity (&gt;30 %) to the present cytochrome c(553) was performed. Analysis, of consensus features accounts for the relatively low reduction potential, as being due to extensive heme hydration and indicates residues 34-35, 44-46, 69-72, and 75 as a conserved hydrophobic patch for the interaction, with a protein partner. At variance with mitochondrial c-type cytochrome, this protein does not experience pH-dependent coordination equilibria. The, reasons for this difference are analyzed.
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<StructureSection load='1k3h' size='340' side='right'caption='[[1k3h]]' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1k3h]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Sporosarcina_pasteurii Sporosarcina pasteurii]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K3H OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1K3H FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HEC:HEME+C'>HEC</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1k3h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1k3h OCA], [https://pdbe.org/1k3h PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1k3h RCSB], [https://www.ebi.ac.uk/pdbsum/1k3h PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1k3h ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/CY553_SPOPA CY553_SPOPA]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/k3/1k3h_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1k3h ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1K3H is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Sporosarcina_pasteurii Sporosarcina pasteurii] with HEC as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1K3H OCA].
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*[[Cytochrome C 3D structures|Cytochrome C 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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NMR solution structure, backbone mobility, and homology modeling of c-type cytochromes from gram-positive bacteria., Banci L, Bertini I, Ciurli S, Dikiy A, Dittmer J, Rosato A, Sciara G, Thompsett AR, Chembiochem. 2002 Apr 2;3(4):299-310. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11933230 11933230]
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[[Category: Large Structures]]
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[[Category: Single protein]]
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[[Category: Sporosarcina pasteurii]]
[[Category: Sporosarcina pasteurii]]
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[[Category: Banci, L.]]
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[[Category: Banci L]]
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[[Category: Bertini, I.]]
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[[Category: Bertini I]]
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[[Category: Ciurli, S.]]
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[[Category: Ciurli S]]
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[[Category: Dikiy, A.]]
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[[Category: Dikiy A]]
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[[Category: Dittmer, J.]]
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[[Category: Dittmer J]]
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[[Category: Rosato, A.]]
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[[Category: Rosato A]]
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[[Category: Sciara, G.]]
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[[Category: Sciara G]]
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[[Category: Thompsett, A.R.]]
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[[Category: Thompsett AR]]
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[[Category: HEC]]
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[[Category: bacillus pasteurii]]
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[[Category: c-553]]
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[[Category: cytochrome]]
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[[Category: electron transfer]]
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[[Category: heme]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 18:49:28 2007''
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Current revision

NMR Solution Structure of Oxidized Cytochrome c-553 from Bacillus pasteurii

PDB ID 1k3h

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