1k4j

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(New page: 200px<br /><applet load="1k4j" size="450" color="white" frame="true" align="right" spinBox="true" caption="1k4j, resolution 2.5&Aring;" /> '''Crystal Structure of ...)
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[[Image:1k4j.jpg|left|200px]]<br /><applet load="1k4j" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1k4j, resolution 2.5&Aring;" />
 
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'''Crystal Structure of the Acyl-homoserinelactone Synthase EsaI Complexed with Rhenate'''<br />
 
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==Overview==
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==Crystal Structure of the Acyl-homoserinelactone Synthase EsaI Complexed with Rhenate==
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Synthesis and detection of acyl-homoserine lactones (AHLs) enables many, gram-negative bacteria to engage in quorum sensing, an intercellular, signaling mechanism that activates differentiation to virulent and biofilm, lifestyles. The AHL synthases catalyze acylation of, S-adenosyl-L-methionine by acyl-acyl carrier protein and lactonization of, the methionine moiety to give AHLs. The crystal structure of the AHL, synthase, EsaI, determined at 1.8 A resolution, reveals a remarkable, structural similarity to the N-acetyltransferases and defines a common, phosphopantetheine binding fold as the catalytic core. Critical residues, responsible for catalysis and acyl chain specificity have been identified, from a modeled substrate complex and verified through functional analysis, in vivo. A mechanism for the N-acylation of S-adenosyl-L-methionine by, 3-oxo-hexanoyl-acyl carrier protein is proposed.
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<StructureSection load='1k4j' size='340' side='right'caption='[[1k4j]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1k4j]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pantoea_stewartii_subsp._stewartii Pantoea stewartii subsp. stewartii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K4J OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1K4J FirstGlance]. <br>
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1K4J is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pantoea_stewartii_subsp._stewartii Pantoea stewartii subsp. stewartii] with REO as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1K4J OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=REO:PERRHENATE'>REO</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1k4j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1k4j OCA], [https://pdbe.org/1k4j PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1k4j RCSB], [https://www.ebi.ac.uk/pdbsum/1k4j PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1k4j ProSAT]</span></td></tr>
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Structural basis and specificity of acyl-homoserine lactone signal production in bacterial quorum sensing., Watson WT, Minogue TD, Val DL, von Bodman SB, Churchill ME, Mol Cell. 2002 Mar;9(3):685-94. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11931774 11931774]
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/ESAI_PANSE ESAI_PANSE] Required for the synthesis of OHHL (N-(3-oxohexanoyl)-L-homoserine lactone), an autoinducer molecule which binds to EsaR. OHHL is necessary for biosynthesis of EPS virulence factor (extracellular heteropolysaccharide) which plays a role in the development of Stewart's wilt on sweet corn.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/k4/1k4j_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1k4j ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Pantoea stewartii subsp. stewartii]]
[[Category: Pantoea stewartii subsp. stewartii]]
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[[Category: Single protein]]
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[[Category: Beck von Bodman S]]
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[[Category: Bodman, S.Beck.von.]]
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[[Category: Churchill MEA]]
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[[Category: Churchill, M.E.A.]]
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[[Category: Minogue TD]]
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[[Category: Minogue, T.D.]]
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[[Category: Val DL]]
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[[Category: Val, D.L.]]
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[[Category: Watson WT]]
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[[Category: Watson, W.T.]]
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[[Category: REO]]
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[[Category: acyl-homoserinelactone synthase]]
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[[Category: autoinducer synthase]]
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[[Category: enzyme]]
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[[Category: gnat similarity]]
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[[Category: mixed alpha beta]]
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[[Category: quorum sensing]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 18:51:18 2007''
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Current revision

Crystal Structure of the Acyl-homoserinelactone Synthase EsaI Complexed with Rhenate

PDB ID 1k4j

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