2b8h

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{{Seed}}
 
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[[Image:2b8h.png|left|200px]]
 
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==A/NWS/whale/Maine/1/84 (H1N9) reassortant influenza virus neuraminidase==
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The line below this paragraph, containing "STRUCTURE_2b8h", creates the "Structure Box" on the page.
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<StructureSection load='2b8h' size='340' side='right'caption='[[2b8h]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2b8h]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Influenza_A_virus Influenza A virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2B8H OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2B8H FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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{{STRUCTURE_2b8h| PDB=2b8h | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2b8h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2b8h OCA], [https://pdbe.org/2b8h PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2b8h RCSB], [https://www.ebi.ac.uk/pdbsum/2b8h PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2b8h ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/NRAM_I84A1 NRAM_I84A1] Catalyzes the removal of terminal sialic acid residues from viral and cellular glycoconjugates. Cleaves off the terminal sialic acids on the glycosylated HA during virus budding to facilitate virus release. Additionally helps virus spread through the circulation by further removing sialic acids from the cell surface. These cleavages prevent self-aggregation and ensure the efficient spread of the progeny virus from cell to cell. Otherwise, infection would be limited to one round of replication. Described as a receptor-destroying enzyme because it cleaves a terminal sialic acid from the cellular receptors. May facilitate viral invasion of the upper airways by cleaving the sialic acid moities on the mucin of the airway epithelial cells. Likely to plays a role in the budding process through its association with lipid rafts during intracellular transport. May additionally display a raft-association independent effect on budding. Plays a role in the determination of host range restriction on replication and virulence. Sialidase activity in late endosome/lysosome traffic seems to enhance virus replication.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/b8/2b8h_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2b8h ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The X-ray structure of influenza virus neuraminidase (NA) isolated from whale, subtype N9, has been determined at 2.2 A resolution and contains a tetrameric protein in the asymmetric unit. In structures of NA determined previously, a calcium ion is observed to coordinate amino acids near the substrate-binding site. In three of the NA monomers determined here this calcium is absent, resulting in structural alterations near the substrate-binding site. These changes affect the conformation of residues that participate in several key interactions between the enzyme and substrate and provide at a molecular level the basis of the structural and functional role of calcium in substrate and inhibitor binding. Several sulfate ions were identified in complex with the protein. These are located in the active site, occupying the space reserved for the substrate (sialic acid) carboxylate, and in positions leading away from the substrate-binding site. These sites offer a new opportunity for the design of inhibitors of influenza virus NA.
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===A/NWS/whale/Maine/1/84 (H1N9) reassortant influenza virus neuraminidase===
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Structure of a calcium-deficient form of influenza virus neuraminidase: implications for substrate binding.,Smith BJ, Huyton T, Joosten RP, McKimm-Breschkin JL, Zhang JG, Luo CS, Lou MZ, Labrou NE, Garrett TP Acta Crystallogr D Biol Crystallogr. 2006 Sep;62(Pt 9):947-52. Epub 2006, Aug 19. PMID:16929094<ref>PMID:16929094</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 2b8h" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_16929094}}, adds the Publication Abstract to the page
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*[[Neuraminidase 3D structures|Neuraminidase 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 16929094 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_16929094}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Influenza A virus]]
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2B8H is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Influenza_a_virus Influenza a virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2B8H OCA].
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[[Category: Large Structures]]
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[[Category: Cox MMJ]]
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==Reference==
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[[Category: Garrett TPJ]]
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Structure of a calcium-deficient form of influenza virus neuraminidase: implications for substrate binding., Smith BJ, Huyton T, Joosten RP, McKimm-Breschkin JL, Zhang JG, Luo CS, Lou MZ, Labrou NE, Garrett TP, Acta Crystallogr D Biol Crystallogr. 2006 Sep;62(Pt 9):947-52. Epub 2006, Aug 19. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16929094 16929094]
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[[Category: Huyton T]]
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[[Category: Exo-alpha-sialidase]]
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[[Category: Joosten RP]]
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[[Category: Influenza a virus]]
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[[Category: Labrou NE]]
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[[Category: Single protein]]
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[[Category: Lou M-Z]]
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[[Category: Cox, M M.J.]]
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[[Category: Luo CS]]
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[[Category: Garrett, T P.J.]]
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[[Category: McKimm-Breschkin JL]]
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[[Category: Huyton, T.]]
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[[Category: Platis D]]
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[[Category: Joosten, R P.]]
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[[Category: Smith BJ]]
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[[Category: Labrou, N E.]]
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[[Category: Zhang J-G]]
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[[Category: Lou, M Z.]]
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[[Category: Luo, C S.]]
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[[Category: McKimm-Breschkin, J L.]]
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[[Category: Platis, D.]]
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[[Category: Smith, B J.]]
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[[Category: Zhang, J G.]]
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[[Category: 6-bladed beta-propeller]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 15:20:41 2008''
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Current revision

A/NWS/whale/Maine/1/84 (H1N9) reassortant influenza virus neuraminidase

PDB ID 2b8h

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