1nf3

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{{Seed}}
 
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[[Image:1nf3.png|left|200px]]
 
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==Structure of Cdc42 in a complex with the GTPase-binding domain of the cell polarity protein, Par6==
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The line below this paragraph, containing "STRUCTURE_1nf3", creates the "Structure Box" on the page.
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<StructureSection load='1nf3' size='340' side='right'caption='[[1nf3]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1nf3]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NF3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1NF3 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GNP:PHOSPHOAMINOPHOSPHONIC+ACID-GUANYLATE+ESTER'>GNP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
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{{STRUCTURE_1nf3| PDB=1nf3 | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1nf3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1nf3 OCA], [https://pdbe.org/1nf3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1nf3 RCSB], [https://www.ebi.ac.uk/pdbsum/1nf3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1nf3 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/CDC42_HUMAN CDC42_HUMAN] Plasma membrane-associated small GTPase which cycles between an active GTP-bound and an inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses. Involved in epithelial cell polarization processes. Regulates the bipolar attachment of spindle microtubules to kinetochores before chromosome congression in metaphase. Plays a role in the extension and maintenance of the formation of thin, actin-rich surface projections called filopodia. Mediates CDC42-dependent cell migration.<ref>PMID:14978216</ref> <ref>PMID:15642749</ref> <ref>PMID:17038317</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/nf/1nf3_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1nf3 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Cdc42 is a small GTPase that is required for cell polarity establishment in eukaryotes as diverse as budding yeast and mammals. Par6 is also implicated in metazoan cell polarity establishment and asymmetric cell divisions. Cdc42.GTP interacts with proteins that contain a conserved sequence called a CRIB motif. Uniquely, Par6 possesses a semi-CRIB motif that is not sufficient for binding to Cdc42. An adjacent PDZ domain is also necessary and is required for biological effects of Par6. Here we report the crystal structure of a complex between Cdc42 and the Par6 GTPase-binding domain. The semi-CRIB motif forms a beta-strand that inserts between the four strands of Cdc42 and the three strands of the PDZ domain to form a continuous eight-stranded sheet. Cdc42 induces a conformational change in Par6, detectable by fluorescence resonance energy transfer spectroscopy. Nuclear magnetic resonance studies indicate that the semi-CRIB motif of Par6 is at least partially structured by the PDZ domain. The structure highlights a novel role for a PDZ domain as a structural scaffold.
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===Structure of Cdc42 in a complex with the GTPase-binding domain of the cell polarity protein, Par6===
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Structure of Cdc42 in a complex with the GTPase-binding domain of the cell polarity protein, Par6.,Garrard SM, Capaldo CT, Gao L, Rosen MK, Macara IG, Tomchick DR EMBO J. 2003 Mar 3;22(5):1125-33. PMID:12606577<ref>PMID:12606577</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 1nf3" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_12606577}}, adds the Publication Abstract to the page
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*[[GTP-binding protein 3D structures|GTP-binding protein 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 12606577 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_12606577}}
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__TOC__
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</StructureSection>
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==About this Structure==
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1NF3 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NF3 OCA].
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==Reference==
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Structure of Cdc42 in a complex with the GTPase-binding domain of the cell polarity protein, Par6., Garrard SM, Capaldo CT, Gao L, Rosen MK, Macara IG, Tomchick DR, EMBO J. 2003 Mar 3;22(5):1125-33. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12606577 12606577]
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[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Large Structures]]
[[Category: Mus musculus]]
[[Category: Mus musculus]]
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[[Category: Protein complex]]
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[[Category: Capaldo CT]]
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[[Category: Capaldo, C T.]]
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[[Category: Gao L]]
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[[Category: Gao, L.]]
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[[Category: Garrard SM]]
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[[Category: Garrard, S M.]]
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[[Category: Macara IG]]
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[[Category: Macara, I G.]]
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[[Category: Rosen MK]]
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[[Category: Rosen, M K.]]
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[[Category: Tomchick DR]]
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[[Category: Tomchick, D R.]]
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[[Category: Gtpase binding domain]]
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[[Category: Pdz domain]]
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[[Category: Semi-crib motif]]
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[[Category: Switch i and ii]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 15:35:44 2008''
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Current revision

Structure of Cdc42 in a complex with the GTPase-binding domain of the cell polarity protein, Par6

PDB ID 1nf3

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