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1k7t

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(New page: 200px<br /><applet load="1k7t" size="450" color="white" frame="true" align="right" spinBox="true" caption="1k7t, resolution 2.4&Aring;" /> '''Crystal Structure Ana...)
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[[Image:1k7t.gif|left|200px]]<br /><applet load="1k7t" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1k7t, resolution 2.4&Aring;" />
 
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'''Crystal Structure Analysis of crosslinked-WGA3/GlcNAcbeta1,6Gal complex'''<br />
 
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==Overview==
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==Crystal Structure Analysis of crosslinked-WGA3/GlcNAcbeta1,6Gal complex==
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The interactions of wheat-germ agglutinin (WGA) with the GlcNAc beta, 1,6Gal sequence, a characteristic component of branched, poly-N-acetyllactosaminoglycans, were investigated using isothermal, titration calorimetry and X-ray crystallography. GlcNAc beta 1,6Gal, exhibited an affinity greater than GlcNAc beta 1,4GlcNAc to all WGA, isolectins, whereas Gal beta 1,6GlcNAc showed much less affinity than, GlcNAc beta 1,4GlcNAc. X-ray structural analyses of the, glutaraldehyde-crosslinked WGA isolectin 3 crystals in complex with GlcNAc, beta 1,6Gal, GlcNAc beta 1,4GlcNAc and GlcNAc beta 1,6Gal beta 1,4Glc were, performed at 2.4, 2.2 and 2.2 A resolution, respectively. In spite of, different glycosidic linkages, GlcNAc beta 1,6Gal and GlcNAc beta, 1,4GlcNAc exhibited basically similar binding modes to each other, in, contact with side chains of two aromatic residues, Tyr64 and His66., However, the conformations of the ligands in the two primary binding sites, were not always identical. GlcNAc beta 1,6Gal showed more extensive, variation in the parameters defining the glycosidic linkage structure, compared to GlcNAc beta 1,4GlcNAc, demonstrating large conformational, flexibility of the former ligand in the interaction with WGA. The, difference in the ligand binding conformation was accompanied by, alterations of the side chain conformation of the amino acid residues, involved in the interactions. The hydrogen bond between Ser62 and the, non-reducing end GlcNAc was always observed regardless of the ligand type, indicating the key role of this interaction. In addition to the hydrogen, bonding and van der Waals interactions, CH--pi interactions involving, Tyr64, His66 and Tyr73 are suggested to play an essential role in, determining the ligand binding conformation in all complexes. One of the, GlcNAc beta 1,6Gal ligands had no crystal packing contact with another WGA, molecule, therefore the conformation might be more relevant to the, interaction mode in solution.
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<StructureSection load='1k7t' size='340' side='right'caption='[[1k7t]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1k7t]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Triticum_aestivum Triticum aestivum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K7T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1K7T FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1k7t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1k7t OCA], [https://pdbe.org/1k7t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1k7t RCSB], [https://www.ebi.ac.uk/pdbsum/1k7t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1k7t ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/AGI3_WHEAT AGI3_WHEAT] N-acetyl-D-glucosamine / N-acetyl-D-neuraminic acid binding lectin.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/k7/1k7t_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1k7t ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1K7T is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Triticum_aestivum Triticum aestivum]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1K7T OCA].
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*[[Agglutinin 3D structures|Agglutinin 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Interactions of wheat-germ agglutinin with GlcNAc beta 1,6Gal sequence., Muraki M, Ishimura M, Harata K, Biochim Biophys Acta. 2002 Jan 15;1569(1-3):10-20. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11853952 11853952]
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[[Category: Large Structures]]
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[[Category: Single protein]]
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[[Category: Triticum aestivum]]
[[Category: Triticum aestivum]]
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[[Category: Harata, K.]]
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[[Category: Harata K]]
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[[Category: Ishimura, M.]]
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[[Category: Ishimura M]]
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[[Category: Muraki, M.]]
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[[Category: Muraki M]]
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[[Category: hevein-type fold]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 18:55:41 2007''
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Current revision

Crystal Structure Analysis of crosslinked-WGA3/GlcNAcbeta1,6Gal complex

PDB ID 1k7t

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