1k8k

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(New page: 200px<br /><applet load="1k8k" size="450" color="white" frame="true" align="right" spinBox="true" caption="1k8k, resolution 2.0&Aring;" /> '''Crystal Structure of ...)
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[[Image:1k8k.gif|left|200px]]<br /><applet load="1k8k" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1k8k, resolution 2.0&Aring;" />
 
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'''Crystal Structure of Arp2/3 Complex'''<br />
 
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==Overview==
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==Crystal Structure of Arp2/3 Complex==
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We determined a crystal structure of bovine Arp2/3 complex, an assembly of, seven proteins that initiates actin polymerization in eukaryotic cells, at, 2.0 angstrom resolution. Actin-related protein 2 (Arp2) and Arp3 are, folded like actin, with distinctive surface features. Subunits ARPC2 p34, and ARPC4 p20 in the core of the complex associate through long, carboxyl-terminal alpha helices and have similarly folded amino-terminal, alpha/beta domains. ARPC1 p40 is a seven-blade beta propeller with an, insertion that may associate with the side of an actin filament. ARPC3 p21, and ARPC5 p16 are globular alpha-helical subunits. We predict that, WASp/Scar proteins activate Arp2/3 complex by bringing Arp2 into proximity, with Arp3 for nucleation of a branch on the side of a preexisting actin, filament.
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<StructureSection load='1k8k' size='340' side='right'caption='[[1k8k]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1k8k]] is a 7 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K8K OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1K8K FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1k8k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1k8k OCA], [https://pdbe.org/1k8k PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1k8k RCSB], [https://www.ebi.ac.uk/pdbsum/1k8k PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1k8k ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/ARP3_BOVIN ARP3_BOVIN] Functions as ATP-binding component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks. Seems to contact the pointed end of the daughter actin filament. Plays a role in ciliogenesis (By similarity).
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/k8/1k8k_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1k8k ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1K8K is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1K8K OCA].
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*[[Actin-related protein 3D structures|Actin-related protein 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Crystal structure of Arp2/3 complex., Robinson RC, Turbedsky K, Kaiser DA, Marchand JB, Higgs HN, Choe S, Pollard TD, Science. 2001 Nov 23;294(5547):1679-84. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11721045 11721045]
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[[Category: Bos taurus]]
[[Category: Bos taurus]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Choe, S.]]
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[[Category: Choe S]]
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[[Category: Higgs, H.N.]]
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[[Category: Higgs HN]]
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[[Category: Kaiser, D.A.]]
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[[Category: Kaiser DA]]
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[[Category: Marchand, J.B.]]
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[[Category: Marchand J-B]]
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[[Category: Pollard, T.D.]]
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[[Category: Pollard TD]]
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[[Category: Robinson, R.C.]]
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[[Category: Robinson RC]]
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[[Category: Turbedsky, K.]]
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[[Category: Turbedsky K]]
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[[Category: beta-propeller]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 18:57:19 2007''
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Current revision

Crystal Structure of Arp2/3 Complex

PDB ID 1k8k

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