1t8t

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{{Seed}}
 
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[[Image:1t8t.png|left|200px]]
 
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==Crystal Structure of human 3-O-Sulfotransferase-3 with bound PAP==
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The line below this paragraph, containing "STRUCTURE_1t8t", creates the "Structure Box" on the page.
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<StructureSection load='1t8t' size='340' side='right'caption='[[1t8t]], [[Resolution|resolution]] 1.85&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1t8t]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T8T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1T8T FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.85&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=A3P:ADENOSINE-3-5-DIPHOSPHATE'>A3P</scene>, <scene name='pdbligand=CIT:CITRIC+ACID'>CIT</scene></td></tr>
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{{STRUCTURE_1t8t| PDB=1t8t | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1t8t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1t8t OCA], [https://pdbe.org/1t8t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1t8t RCSB], [https://www.ebi.ac.uk/pdbsum/1t8t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1t8t ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/HS3SA_HUMAN HS3SA_HUMAN] Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) to catalyze the transfer of a sulfo group to an N-unsubstituted glucosamine linked to a 2-O-sulfo iduronic acid unit on heparan sulfate. Catalyzes the O-sulfation of glucosamine in IdoUA2S-GlcNS and also in IdoUA2S-GlcNH2. The substrate-specific O-sulfation generates an enzyme-modified heparan sulfate which acts as a binding receptor to Herpes simplex virus-1 (HSV-1) and permits its entry. Unlike 3-OST-1, does not convert non-anticoagulant heparan sulfate to anticoagulant heparan sulfate.<ref>PMID:10520990</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/t8/1t8t_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1t8t ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Heparan sulfate (HS) plays essential roles in assisting herpes simplex virus infection and other biological processes. The biosynthesis of HS includes numerous specialized sulfotransferases that generate a variety of sulfated saccharide sequences, conferring the selectivity of biological functions of HS. We report a structural study of human HS 3-O-sulfotransferase isoform 3 (3-OST-3), a key sulfotransferase that transfers a sulfuryl group to a specific glucosamine in HS generating an entry receptor for herpes simplex virus 1. We have obtained the crystal structure of 3-OST-3 at 1.95 A in a ternary complex with 3'-phosphoadenosine 5'-phosphate and a tetrasaccharide substrate. Mutational analyses were also performed on the residues involved in the binding of the substrate. Residues Gln255 and Lys368 are essential for the sulfotransferase activity and lie within hydrogen bonding distances to the carboxyl and sulfo groups of the uronic acid unit. These residues participate in the substrate recognition of 3-OST-3. This structure provides atomic level evidence for delineating the substrate recognition and catalytic mechanism for 3-OST-3.
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===Crystal Structure of human 3-O-Sulfotransferase-3 with bound PAP===
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Structural analysis of the sulfotransferase (3-o-sulfotransferase isoform 3) involved in the biosynthesis of an entry receptor for herpes simplex virus 1.,Moon AF, Edavettal SC, Krahn JM, Munoz EM, Negishi M, Linhardt RJ, Liu J, Pedersen LC J Biol Chem. 2004 Oct 22;279(43):45185-93. Epub 2004 Aug 10. PMID:15304505<ref>PMID:15304505</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 1t8t" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_15304505}}, adds the Publication Abstract to the page
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*[[Sulfotransferase 3D structures|Sulfotransferase 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 15304505 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_15304505}}
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__TOC__
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</StructureSection>
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==About this Structure==
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1T8T is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T8T OCA].
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==Reference==
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Structural analysis of the sulfotransferase (3-o-sulfotransferase isoform 3) involved in the biosynthesis of an entry receptor for herpes simplex virus 1., Moon AF, Edavettal SC, Krahn JM, Munoz EM, Negishi M, Linhardt RJ, Liu J, Pedersen LC, J Biol Chem. 2004 Oct 22;279(43):45185-93. Epub 2004 Aug 10. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15304505 15304505]
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[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Edavettal, S C.]]
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[[Category: Edavettal SC]]
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[[Category: Krahn, J M.]]
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[[Category: Krahn JM]]
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[[Category: Linhardt, R J.]]
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[[Category: Linhardt RJ]]
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[[Category: Liu, J.]]
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[[Category: Liu J]]
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[[Category: Moon, A F.]]
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[[Category: Moon AF]]
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[[Category: Munoz, E M.]]
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[[Category: Munoz EM]]
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[[Category: Negishi, M.]]
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[[Category: Negishi M]]
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[[Category: Pedersen, L C.]]
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[[Category: Pedersen LC]]
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[[Category: Alpha-beta motif]]
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[[Category: Substrate-binding cleft]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 16:03:13 2008''
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Current revision

Crystal Structure of human 3-O-Sulfotransferase-3 with bound PAP

PDB ID 1t8t

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