1ka9

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(New page: 200px<br /><applet load="1ka9" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ka9, resolution 2.3&Aring;" /> '''Imidazole Glycerol Ph...)
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[[Image:1ka9.jpg|left|200px]]<br /><applet load="1ka9" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1ka9, resolution 2.3&Aring;" />
 
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'''Imidazole Glycerol Phosphate Synthase'''<br />
 
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==Overview==
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==Imidazole Glycerol Phosphate Synthase==
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Imidazole glycerol phosphate synthase (IGPs) catalyzes the fifth step in, the histidine biosynthetic pathway located at the branch point to de novo, purine biosynthesis. IGPs is a multienzyme comprising glutaminase and, synthase subunits. The glutaminase activity, which hydrolyzes glutamine to, give ammonia, is coupled with substrate binding to the synthase subunit., The three-dimensional structure of the IGPs from Thermus thermophilus HB8, has been determined at 2.3 A resolution, and compared with the previously, determined structures for the yeast and Thermotoga maritima enzymes. The, structure of each subunit is similar to that of the corresponding domain, in the yeast enzyme or subunit in the T. maritima enzyme. However, the, overall structure is significantly different from the yeast and T., maritima enzymes, indicating that IGPs may change the relative orientation, between the two subunits and close the glutaminase site upon glutamine, binding. The putative ammonia tunnel, which carries nascent ammonia from, glutaminase to the synthase site, has a closed gate comprising a cyclic, salt bridge formed by four charged residues of the synthase subunit. The, side chain of Lys100 in the cyclic salt bridge might change its side chain, direction to form new interactions with the main chain carbonyl group of, glutamine from the synthase subunit and the hydoxyl group of tyrosine from, the glutaminase subunit, resulting in the opening of the gate for ammonia, transfer.
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<StructureSection load='1ka9' size='340' side='right'caption='[[1ka9]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1ka9]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KA9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1KA9 FirstGlance]. <br>
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1KA9 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1KA9 OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ka9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ka9 OCA], [https://pdbe.org/1ka9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ka9 RCSB], [https://www.ebi.ac.uk/pdbsum/1ka9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ka9 ProSAT], [https://www.topsan.org/Proteins/RSGI/1ka9 TOPSAN]</span></td></tr>
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==Reference==
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</table>
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Structure of imidazole glycerol phosphate synthase from Thermus thermophilus HB8: open-closed conformational change and ammonia tunneling., Omi R, Mizuguchi H, Goto M, Miyahara I, Hayashi H, Kagamiyama H, Hirotsu K, J Biochem (Tokyo). 2002 Nov;132(5):759-65. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12417026 12417026]
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== Function ==
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[[Category: Protein complex]]
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[https://www.uniprot.org/uniprot/HIS5_THET8 HIS5_THET8] IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The HisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to HisF for the synthesis of IGP and AICAR (By similarity).[HAMAP-Rule:MF_00278]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ka/1ka9_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ka9 ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Thermus thermophilus]]
[[Category: Thermus thermophilus]]
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[[Category: Hirotsu, K.]]
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[[Category: Hirotsu K]]
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[[Category: Miyahara, I.]]
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[[Category: Miyahara I]]
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[[Category: Omi, R.]]
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[[Category: Omi R]]
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[[Category: RSGI, RIKEN.Structural.Genomics/Proteomics.Initiative.]]
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[[Category: riken structural genomics/proteomics initiative]]
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[[Category: rsgi]]
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[[Category: structural genomics]]
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[[Category: synthase]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 18:59:44 2007''
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Current revision

Imidazole Glycerol Phosphate Synthase

PDB ID 1ka9

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