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2cm6

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(New page: 200px<br /> <applet load="2cm6" size="450" color="white" frame="true" align="right" spinBox="true" caption="2cm6, resolution 1.85&Aring;" /> '''CRYSTAL STRUCTURE O...)
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[[Image:2cm6.gif|left|200px]]<br />
 
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<applet load="2cm6" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="2cm6, resolution 1.85&Aring;" />
 
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'''CRYSTAL STRUCTURE OF THE C2B DOMAIN OF RABPHILIN3A'''<br />
 
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==Overview==
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==crystal structure of the C2B domain of rabphilin3A==
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The Ca(2+) binding properties of C2 domains are essential for the function, of their host proteins. We present here the first crystal structures, showing an unexpected Ca(2+) binding mode of the C2B domain of, rabphilin-3A in atomic detail. Acidic residues from the linker region, between the C2A and C2B domains of rabphilin-3A interact with the, Ca(2+)-binding region of the C2B domain. Because of these interactions, the coordination sphere of the two bound Ca(2+) ions is almost complete., Mutation of these acidic residues to alanine resulted in a 10-fold, decrease in the intrinsic Ca(2+) binding affinity of the C2B domain. Using, NMR spectroscopy, we show that this interaction occurred only in the, Ca(2+)-bound state of the C2B domain. In addition, this Ca(2+) binding, mode was maintained ... [[http://ispc.weizmann.ac.il/pmbin/getpm?17166855 (full description)]]
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<StructureSection load='2cm6' size='340' side='right'caption='[[2cm6]], [[Resolution|resolution]] 1.85&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2cm6]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CM6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2CM6 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.85&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2cm6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cm6 OCA], [https://pdbe.org/2cm6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2cm6 RCSB], [https://www.ebi.ac.uk/pdbsum/2cm6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2cm6 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/RP3A_RAT RP3A_RAT] Protein transport. Probably involved with Ras-related protein Rab-3A in synaptic vesicle traffic and/or synaptic vesicle fusion. Could play a role in neurotransmitter release by regulating membrane flow in the nerve terminal.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cm/2cm6_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2cm6 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The Ca(2+) binding properties of C2 domains are essential for the function of their host proteins. We present here the first crystal structures showing an unexpected Ca(2+) binding mode of the C2B domain of rabphilin-3A in atomic detail. Acidic residues from the linker region between the C2A and C2B domains of rabphilin-3A interact with the Ca(2+)-binding region of the C2B domain. Because of these interactions, the coordination sphere of the two bound Ca(2+) ions is almost complete. Mutation of these acidic residues to alanine resulted in a 10-fold decrease in the intrinsic Ca(2+) binding affinity of the C2B domain. Using NMR spectroscopy, we show that this interaction occurred only in the Ca(2+)-bound state of the C2B domain. In addition, this Ca(2+) binding mode was maintained in the C2 domain tandem fragment. In NMR-based liposome binding assays, the linker was not released upon phospholipid binding. Therefore, this unprecedented Ca(2+) binding mode not only shows how a C2 domain increases its intrinsic Ca(2+) affinity, but also provides the structural base for an atypical protein-Ca(2+)-phospholipid binding mode of rabphilin-3A.
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==About this Structure==
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The C2A-C2B linker defines the high affinity Ca(2+) binding mode of rabphilin-3A.,Montaville P, Schlicker C, Leonov A, Zweckstetter M, Sheldrick GM, Becker S J Biol Chem. 2007 Feb 16;282(7):5015-25. Epub 2006 Dec 13. PMID:17166855<ref>PMID:17166855</ref>
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2CM6 is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]] with CA and PO4 as [[http://en.wikipedia.org/wiki/ligands ligands]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2CM6 OCA]].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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The C2A-C2B linker defines the high affinity Ca(2+) binding mode of rabphilin-3A., Montaville P, Schlicker C, Leonov A, Zweckstetter M, Sheldrick GM, Becker S, J Biol Chem. 2007 Feb 16;282(7):5015-25. Epub 2006 Dec 13. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17166855 17166855]
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</div>
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[[Category: Rattus norvegicus]]
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<div class="pdbe-citations 2cm6" style="background-color:#fffaf0;"></div>
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[[Category: Single protein]]
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[[Category: Becker, S.]]
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[[Category: Montaville, P.]]
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[[Category: Schlicker, C.]]
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[[Category: Sheldrick, G.M.]]
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[[Category: CA]]
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[[Category: PO4]]
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[[Category: c2 domain]]
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[[Category: c2a-c2b linker fragment]]
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[[Category: c2b]]
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[[Category: ca2+ binding]]
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[[Category: metal-binding]]
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[[Category: protein transport]]
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[[Category: rabphilin3a]]
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[[Category: synapse]]
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[[Category: synaptic exocytosis]]
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[[Category: transport]]
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[[Category: zinc]]
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[[Category: zinc-finger]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Oct 29 20:12:57 2007''
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==See Also==
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*[[Exophilin 3D structures|Exophilin 3D structures]]
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*[[Rabphilin|Rabphilin]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
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[[Category: Rattus norvegicus]]
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[[Category: Becker S]]
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[[Category: Montaville P]]
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[[Category: Schlicker C]]
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[[Category: Sheldrick GM]]

Current revision

crystal structure of the C2B domain of rabphilin3A

PDB ID 2cm6

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