3bsb

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (14:50, 1 November 2023) (edit) (undo)
 
(8 intermediate revisions not shown.)
Line 1: Line 1:
-
{{Seed}}
 
-
[[Image:3bsb.png|left|200px]]
 
-
<!--
+
==Crystal Structure of Human Pumilio1 in Complex with CyclinB reverse RNA==
-
The line below this paragraph, containing "STRUCTURE_3bsb", creates the "Structure Box" on the page.
+
<StructureSection load='3bsb' size='340' side='right'caption='[[3bsb]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
-
You may change the PDB parameter (which sets the PDB file loaded into the applet)
+
== Structural highlights ==
-
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+
<table><tr><td colspan='2'>[[3bsb]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BSB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3BSB FirstGlance]. <br>
-
or leave the SCENE parameter empty for the default display.
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
-
-->
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3bsb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3bsb OCA], [https://pdbe.org/3bsb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3bsb RCSB], [https://www.ebi.ac.uk/pdbsum/3bsb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3bsb ProSAT]</span></td></tr>
-
{{STRUCTURE_3bsb| PDB=3bsb | SCENE= }}
+
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/PUM1_HUMAN PUM1_HUMAN] Sequence-specific RNA-binding protein that regulates translation and mRNA stability by binding the 3'-UTR of mRNA targets. May be required to support proliferation and self-renewal of stem cells (By similarity).
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bs/3bsb_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3bsb ConSurf].
 +
<div style="clear:both"></div>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
Pumilio is a founder member of the evolutionarily conserved Puf family of RNA-binding proteins that control a number of physiological processes in eukaryotes. A structure of human Pumilio (hPum) Puf domain bound to a Drosophila regulatory sequence showed that each Puf repeat recognizes a single nucleotide. Puf domains in general bind promiscuously to a large set of degenerate sequences, but the structural basis for this promiscuity has been unclear. Here, we describe the structures of hPum Puf domain complexed to two noncognate RNAs, CycB(reverse) and Puf5. In each complex, one of the nucleotides is ejected from the binding surface, in effect, acting as a "spacer." The complexes also reveal the plasticity of several Puf repeats, which recognize noncanonical nucleotides. Together, these complexes provide a molecular basis for recognition of degenerate binding sites, which significantly increases the number of mRNAs targeted for regulation by Puf proteins in vivo.
-
===Crystal Structure of Human Pumilio1 in Complex with CyclinB reverse RNA===
+
Structures of human Pumilio with noncognate RNAs reveal molecular mechanisms for binding promiscuity.,Gupta YK, Nair DT, Wharton RP, Aggarwal AK Structure. 2008 Apr;16(4):549-57. Epub 2008 Mar 6. PMID:18328718<ref>PMID:18328718</ref>
-
 
+
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-
<!--
+
</div>
-
The line below this paragraph, {{ABSTRACT_PUBMED_18328718}}, adds the Publication Abstract to the page
+
<div class="pdbe-citations 3bsb" style="background-color:#fffaf0;"></div>
-
(as it appears on PubMed at http://www.pubmed.gov), where 18328718 is the PubMed ID number.
+
== References ==
-
-->
+
<references/>
-
{{ABSTRACT_PUBMED_18328718}}
+
__TOC__
-
 
+
</StructureSection>
-
==About this Structure==
+
-
3BSB is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BSB OCA].
+
-
 
+
-
==Reference==
+
-
Structures of Human Pumilio with Noncognate RNAs Reveal Molecular Mechanisms for Binding Promiscuity., Gupta YK, Nair DT, Wharton RP, Aggarwal AK, Structure. 2008 Mar 5;. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18328718 18328718]
+
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
-
[[Category: Protein complex]]
+
[[Category: Large Structures]]
-
[[Category: Aggarwal, A K.]]
+
[[Category: Aggarwal AK]]
-
[[Category: Gupta, Y K.]]
+
[[Category: Gupta YK]]
-
[[Category: Nair, D T.]]
+
[[Category: Nair DT]]
-
[[Category: Wharton, R P.]]
+
[[Category: Wharton RP]]
-
[[Category: Alternative splicing]]
+
-
[[Category: Cytoplasm]]
+
-
[[Category: Phosphoprotein]]
+
-
[[Category: Protein-rna complex]]
+
-
[[Category: Rna binding protein/rna complex]]
+
-
[[Category: Rna-binding]]
+
-
[[Category: Translation regulation]]
+
-
 
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 16:35:11 2008''
+

Current revision

Crystal Structure of Human Pumilio1 in Complex with CyclinB reverse RNA

PDB ID 3bsb

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3bsb"
Personal tools