This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


1key

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
(New page: 200px<br /><applet load="1key" size="450" color="white" frame="true" align="right" spinBox="true" caption="1key, resolution 2.65&Aring;" /> '''Crystal Structure of...)
Current revision (07:46, 7 February 2024) (edit) (undo)
 
(15 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1key.gif|left|200px]]<br /><applet load="1key" size="450" color="white" frame="true" align="right" spinBox="true"
 
-
caption="1key, resolution 2.65&Aring;" />
 
-
'''Crystal Structure of Mouse Testis/Brain RNA-binding Protein (TB-RBP)'''<br />
 
-
==Overview==
+
==Crystal Structure of Mouse Testis/Brain RNA-binding Protein (TB-RBP)==
-
The testis/brain-RNA-binding protein (TB-RBP) spatially and temporally, controls the expression of specific mRNAs in developing male germ cells, and brain cells, and is implicated in DNA recombination and repair events., We report the 2.65 A crystal structure of mouse TB-RBP. The structure is, predominantly alpha-helical and exhibits a novel protein fold and mode of, assembly. Crystal symmetry and molecular symmetry combine to form an octet, of TB-RBP monomers in the shape of an elongated spherical particle with a, large cavity at its center. Amino acid residues that affect RNA and DNA, binding are located on the interior surface of the assembled particle, and, a putative nucleotide-binding domain that controls RNA binding is located, at a dimer interface. Other modes of assembly are suggested for TB-RBP, based on our structure and recently reported electron microscopic, reconstructions of human TB-RBP.
+
<StructureSection load='1key' size='340' side='right'caption='[[1key]], [[Resolution|resolution]] 2.65&Aring;' scene=''>
-
 
+
== Structural highlights ==
-
==About this Structure==
+
<table><tr><td colspan='2'>[[1key]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KEY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1KEY FirstGlance]. <br>
-
1KEY is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1KEY OCA].
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.65&#8491;</td></tr>
-
 
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1key FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1key OCA], [https://pdbe.org/1key PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1key RCSB], [https://www.ebi.ac.uk/pdbsum/1key PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1key ProSAT]</span></td></tr>
-
==Reference==
+
</table>
-
Crystal structure of TB-RBP, a novel RNA-binding and regulating protein., Pascal JM, Hart PJ, Hecht NB, Robertus JD, J Mol Biol. 2002 Jun 21;319(5):1049-57. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12079346 12079346]
+
== Function ==
 +
[https://www.uniprot.org/uniprot/TSN_MOUSE TSN_MOUSE] DNA-binding protein that specifically recognizes consensus sequences at the breakpoint junctions in chromosomal translocations, mostly involving immunoglobulin (Ig)/T-cell receptor gene segments. Seems to recognize single-stranded DNA ends generated by staggered breaks occurring at recombination hot spots.<ref>PMID:15491149</ref> Exhibits both single-stranded and double-stranded endoribonuclease activity. May act as an activator of RNA-induced silencing complex (RISC) by facilitating endonucleolytic cleavage of the siRNA passenger strand.<ref>PMID:15491149</ref>
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ke/1key_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1key ConSurf].
 +
<div style="clear:both"></div>
 +
== References ==
 +
<references/>
 +
__TOC__
 +
</StructureSection>
 +
[[Category: Large Structures]]
[[Category: Mus musculus]]
[[Category: Mus musculus]]
-
[[Category: Single protein]]
+
[[Category: Hart PJ]]
-
[[Category: Hart, P.J.]]
+
[[Category: Hecht NB]]
-
[[Category: Hecht, N.B.]]
+
[[Category: Pascal JM]]
-
[[Category: Pascal, J.M.]]
+
[[Category: Robertus JD]]
-
[[Category: Robertus, J.D.]]
+
-
[[Category: apotb-rbp]]
+
-
[[Category: rna-binding protein]]
+
-
[[Category: tetramer/octamer assembly]]
+
-
 
+
-
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 19:08:36 2007''
+

Current revision

Crystal Structure of Mouse Testis/Brain RNA-binding Protein (TB-RBP)

PDB ID 1key

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1key"
Personal tools