1kh8

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(New page: 200px<br /><applet load="1kh8" size="450" color="white" frame="true" align="right" spinBox="true" caption="1kh8, resolution 2.0&Aring;" /> '''Structure of a cis-pr...)
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[[Image:1kh8.gif|left|200px]]<br /><applet load="1kh8" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1kh8, resolution 2.0&Aring;" />
 
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'''Structure of a cis-proline (P114) to glycine variant of Ribonuclease A'''<br />
 
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==Overview==
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==Structure of a cis-proline (P114) to glycine variant of Ribonuclease A==
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Replacement of a cis-proline by glycine at position 114 in ribonuclease A, leads to a large decrease in thermal stability and simplifies the, refolding kinetics. A crystallographic approach was used to determine, whether the decrease in thermal stability results from the presence of a, cis glycine peptide bond, or from a localized structural rearrangement, caused by the isomerization of the mutated cis 114 peptide bond. The, structure was solved at 2.0 A resolution and refined to an R-factor of, 19.5% and an R(free) of 21.9%. The overall conformation of the protein was, similar to that of wild-type ribonuclease A; however, there was a large, localized rearrangement of the mutated loop (residues 110-117-a 9.3 A, shift of the Calpha atom of residue 114). The peptide bond before Gly114, is in the trans configuration. Interestingly, a large anomalous difference, density was found near residue 114, and was attributed to a bound cesium, ion present in the crystallization experiment. The trans isomeric, configuration of the peptide bond in the folded state of this mutant is, consistent with the refolding kinetics previously reported, and the, associated protein conformational change provides an explanation for the, decreased thermal stability.
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<StructureSection load='1kh8' size='340' side='right'caption='[[1kh8]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1kh8]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KH8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1KH8 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CS:CESIUM+ION'>CS</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1kh8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1kh8 OCA], [https://pdbe.org/1kh8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1kh8 RCSB], [https://www.ebi.ac.uk/pdbsum/1kh8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1kh8 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/RNAS1_BOVIN RNAS1_BOVIN] Endonuclease that catalyzes the cleavage of RNA on the 3' side of pyrimidine nucleotides. Acts on single stranded and double stranded RNA.<ref>PMID:7479688</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/kh/1kh8_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1kh8 ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1KH8 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] with SO4 and CS as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Pancreatic_ribonuclease Pancreatic ribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.5 3.1.27.5] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1KH8 OCA].
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*[[Ribonuclease 3D structures|Ribonuclease 3D structures]]
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== References ==
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==Reference==
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<references/>
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The crystal structure of the cis-proline to glycine variant (P114G) of ribonuclease A., Schultz DA, Friedman AM, White MA, Fox RO, Protein Sci. 2005 Nov;14(11):2862-70. Epub 2005 Sep 30. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16199662 16199662]
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__TOC__
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</StructureSection>
[[Category: Bos taurus]]
[[Category: Bos taurus]]
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[[Category: Pancreatic ribonuclease]]
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[[Category: Large Structures]]
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[[Category: Single protein]]
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[[Category: Fox RO]]
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[[Category: Fox, R.O.]]
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[[Category: Friedman AM]]
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[[Category: Friedman, A.M.]]
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[[Category: Schultz DA]]
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[[Category: Schultz, D.A.]]
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[[Category: White MA]]
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[[Category: White, M.A.]]
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[[Category: CS]]
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[[Category: SO4]]
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[[Category: cesium]]
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[[Category: cis]]
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[[Category: proline]]
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[[Category: ribonuclease a]]
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[[Category: rnase a]]
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[[Category: trans]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 19:12:47 2007''
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Current revision

Structure of a cis-proline (P114) to glycine variant of Ribonuclease A

PDB ID 1kh8

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