2iw3

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{{Seed}}
 
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[[Image:2iw3.png|left|200px]]
 
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==Elongation Factor 3 in complex with ADP==
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The line below this paragraph, containing "STRUCTURE_2iw3", creates the "Structure Box" on the page.
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<StructureSection load='2iw3' size='340' side='right'caption='[[2iw3]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2iw3]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IW3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2IW3 FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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{{STRUCTURE_2iw3| PDB=2iw3 | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2iw3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2iw3 OCA], [https://pdbe.org/2iw3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2iw3 RCSB], [https://www.ebi.ac.uk/pdbsum/2iw3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2iw3 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/EF3A_YEAST EF3A_YEAST] Required for the ATP-dependent release of deacylated tRNA from the ribosomal E-site during protein biosynthesis. Stimulates the eEF1A-dependent binding of aminoacyl-tRNA to the ribosomal A-site, which has reduced affinity for tRNA as long as the E-site is occupied.<ref>PMID:6456269</ref> <ref>PMID:7657623</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/iw/2iw3_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2iw3 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Elongation factor eEF3 is an ATPase that, in addition to the two canonical factors eEF1A and eEF2, serves an essential function in the translation cycle of fungi. eEF3 is required for the binding of the aminoacyl-tRNA-eEF1A-GTP ternary complex to the ribosomal A-site and has been suggested to facilitate the clearance of deacyl-tRNA from the E-site. Here we present the crystal structure of Saccharomyces cerevisiae eEF3, showing that it consists of an amino-terminal HEAT repeat domain, followed by a four-helix bundle and two ABC-type ATPase domains, with a chromodomain inserted in ABC2. Moreover, we present the cryo-electron microscopy structure of the ATP-bound form of eEF3 in complex with the post-translocational-state 80S ribosome from yeast. eEF3 uses an entirely new factor binding site near the ribosomal E-site, with the chromodomain likely to stabilize the ribosomal L1 stalk in an open conformation, thus allowing tRNA release.
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===ELONGATION FACTOR 3 IN COMPLEX WITH ADP===
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Structure of eEF3 and the mechanism of transfer RNA release from the E-site.,Andersen CB, Becker T, Blau M, Anand M, Halic M, Balar B, Mielke T, Boesen T, Pedersen JS, Spahn CM, Kinzy TG, Andersen GR, Beckmann R Nature. 2006 Oct 12;443(7112):663-8. Epub 2006 Aug 23. PMID:16929303<ref>PMID:16929303</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 2iw3" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_16929303}}, adds the Publication Abstract to the page
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*[[Elongation factor 3D structures|Elongation factor 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 16929303 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_16929303}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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2IW3 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IW3 OCA].
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==Reference==
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Structure of eEF3 and the mechanism of transfer RNA release from the E-site., Andersen CB, Becker T, Blau M, Anand M, Halic M, Balar B, Mielke T, Boesen T, Pedersen JS, Spahn CM, Kinzy TG, Andersen GR, Beckmann R, Nature. 2006 Oct 12;443(7112):663-8. Epub 2006 Aug 23. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16929303 16929303]
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[[Category: Saccharomyces cerevisiae]]
[[Category: Saccharomyces cerevisiae]]
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[[Category: Single protein]]
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[[Category: Anand M]]
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[[Category: Anand, M.]]
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[[Category: Andersen CBF]]
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[[Category: Andersen, C B.F.]]
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[[Category: Andersen GR]]
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[[Category: Andersen, G R.]]
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[[Category: Balar B]]
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[[Category: Balar, B.]]
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[[Category: Becker T]]
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[[Category: Becker, T.]]
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[[Category: Beckmann R]]
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[[Category: Beckmann, R.]]
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[[Category: Blau M]]
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[[Category: Blau, M.]]
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[[Category: Boesen T]]
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[[Category: Boesen, T.]]
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[[Category: Halic M]]
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[[Category: Halic, M.]]
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[[Category: Kinzy TG]]
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[[Category: Kinzy, T G.]]
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[[Category: Mielke T]]
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[[Category: Mielke, T.]]
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[[Category: Pedersen JS]]
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[[Category: Pedersen, J S.]]
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[[Category: Spahn CMT]]
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[[Category: Spahn, C M.T.]]
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[[Category: Acetylation]]
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[[Category: Atp-binding]]
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[[Category: Elongation factor]]
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[[Category: Nucleotide-binding]]
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[[Category: Phosphorylation]]
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[[Category: Protein biosynthesis]]
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[[Category: Rna-binding]]
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[[Category: Rrna-binding]]
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[[Category: Translation]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 17:28:34 2008''
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Current revision

Elongation Factor 3 in complex with ADP

PDB ID 2iw3

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