1klk

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(New page: 200px<br /><applet load="1klk" size="450" color="white" frame="true" align="right" spinBox="true" caption="1klk, resolution 2.3&Aring;" /> '''CRYSTAL STRUCTURE OF ...)
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[[Image:1klk.gif|left|200px]]<br /><applet load="1klk" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1klk, resolution 2.3&Aring;" />
 
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'''CRYSTAL STRUCTURE OF PNEUMOCYSTIS CARINII DIHYDROFOLATE REDUCTASE TERNARY COMPLEX WITH PT653 AND NADPH'''<br />
 
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==Overview==
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==CRYSTAL STRUCTURE OF PNEUMOCYSTIS CARINII DIHYDROFOLATE REDUCTASE TERNARY COMPLEX WITH PT653 AND NADPH==
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Structural data are reported for, N-(2,4-diaminopteridin-6-yl)methyldibenz[b,f]azepine (PT653), an example, of structure-based inhibitor design with 21-fold selectivity for, Pneumocystis carinii dihydrofolate reductase (pcDHFR) relative to rat, liver dihydrofolate reductase (rlDHFR). These data test the hypothesis, that 2,4-diaminopteridines with a bulky N,N-diarylaminomethyl side chain, at the 6-position could fit better into the larger active site of pcDHFR, than into that of mammalian DHFR. The crystal structure of the ternary, complex of NADPH, PT653 and pcDHFR, refined to 2.4 A resolution, reveals, that PT653 binds in a different orientation than predicted from modeling, studies reported previously [Rosowsky et al. (1999), J. Med. Chem. 42, 4853-4860]. These crystal data show that the pteridine-ring plane is, tilted compared with that observed in the crystal structure of the pcDHFR, methotrexate (MTX) NADPH ternary complex used as a template to model PT653, binding. Also, as a result of this tilt, the dibenzoazepine ring is bound, deeper into the p-aminobenzoyl folate binding pocket of pcDHFR, thereby, relieving close intermolecular contacts predicted from the modeling data., By far the most significant structural change, but more subtle in, magnitude, is the ligand-induced conformational shift of 1.2 A away from, the inhibitor of residues 61-66 in helix C. The other major effect is the, unwinding of the short helical segment involving loop 47 which has a, different conformation to that observed in other pcDHFR complexes [Cody et, al. (1999), Biochemistry, 38, 4303-4312]. The favorable pcDHFR selectivity, of PT653 could be a result of ligand-induced fit of the large hydrophobic, dibenzazepine ring which occupies regions of the enzyme active site not, probed by other antifolates and which take advantage of sequence and, conformational differences between the structures of human and pcDHFR., These data suggest that such hydrophobic analogs could be used as lead, compounds in the design of more pcDHFR-selective antifolates. Enzyme, inhibition data also show that PT653 is 102-fold selective for Toxoplasma, gondii (tg) DHFR relative to rlDHFR. Homology-modeling studies of the, tgDHFR structure suggest that differences in ligand-binding orientation, and enzyme sequence could influence the enhanced selectivity of PT653 for, tgDHFR.
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<StructureSection load='1klk' size='340' side='right'caption='[[1klk]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1klk]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pneumocystis_carinii Pneumocystis carinii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KLK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1KLK FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NDP:NADPH+DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NDP</scene>, <scene name='pdbligand=PMD:[N-(2,4-DIAMINOPTERIDIN-6-YL)-METHYL]-DIBENZ[B,F]AZEPINE'>PMD</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1klk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1klk OCA], [https://pdbe.org/1klk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1klk RCSB], [https://www.ebi.ac.uk/pdbsum/1klk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1klk ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/DYR_PNECA DYR_PNECA] Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/kl/1klk_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1klk ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1KLK is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus] with PMD and NDP as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Dihydrofolate_reductase Dihydrofolate reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.5.1.3 1.5.1.3] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1KLK OCA].
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*[[Dihydrofolate reductase 3D structures|Dihydrofolate reductase 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Structure-based enzyme inhibitor design: modeling studies and crystal structure analysis of Pneumocystis carinii dihydrofolate reductase ternary complex with PT653 and NADPH., Cody V, Galitsky N, Luft JR, Pangborn W, Rosowsky A, Queener SF, Acta Crystallogr D Biol Crystallogr. 2002 Jun;58(Pt 6 Pt 2):946-54. Epub, 2002 May 29. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12037296 12037296]
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[[Category: Large Structures]]
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[[Category: Dihydrofolate reductase]]
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[[Category: Pneumocystis carinii]]
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[[Category: Rattus norvegicus]]
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[[Category: Cody V]]
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[[Category: Single protein]]
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[[Category: Galitsky N]]
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[[Category: Cody, V.]]
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[[Category: Luft JR]]
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[[Category: Galitsky, N.]]
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[[Category: Pangborn W]]
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[[Category: Luft, J.R.]]
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[[Category: Queener SF]]
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[[Category: Pangborn, W.]]
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[[Category: Rosowsky A]]
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[[Category: Queener, S.F.]]
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[[Category: Rosowsky, A.]]
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[[Category: NDP]]
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[[Category: PMD]]
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[[Category: aids]]
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[[Category: antifolate]]
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[[Category: protein-inhibitor complex]]
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[[Category: structure-based design]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 19:20:41 2007''
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Current revision

CRYSTAL STRUCTURE OF PNEUMOCYSTIS CARINII DIHYDROFOLATE REDUCTASE TERNARY COMPLEX WITH PT653 AND NADPH

PDB ID 1klk

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