1n3e

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{{Seed}}
 
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[[Image:1n3e.png|left|200px]]
 
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==Crystal structure of I-CreI bound to a palindromic DNA sequence I (palindrome of left side of wildtype DNA target sequence)==
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The line below this paragraph, containing "STRUCTURE_1n3e", creates the "Structure Box" on the page.
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<StructureSection load='1n3e' size='340' side='right'caption='[[1n3e]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1n3e]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Chlamydomonas_reinhardtii Chlamydomonas reinhardtii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N3E OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1N3E FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
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{{STRUCTURE_1n3e| PDB=1n3e | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1n3e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1n3e OCA], [https://pdbe.org/1n3e PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1n3e RCSB], [https://www.ebi.ac.uk/pdbsum/1n3e PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1n3e ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/DNE1_CHLRE DNE1_CHLRE] Endonuclease involved in group I intron homing. Recognizes and cleaves a 19-24 bp palindromic DNA site.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/n3/1n3e_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1n3e ConSurf].
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<div style="clear:both"></div>
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===Crystal structure of I-CreI bound to a palindromic DNA sequence I (palindrome of left side of wildtype DNA target sequence)===
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==See Also==
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*[[Endonuclease 3D structures|Endonuclease 3D structures]]
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__TOC__
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</StructureSection>
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The line below this paragraph, {{ABSTRACT_PUBMED_12758074}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 12758074 is the PubMed ID number.
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{{ABSTRACT_PUBMED_12758074}}
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==About this Structure==
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1N3E is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Chlamydomonas_reinhardtii Chlamydomonas reinhardtii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N3E OCA].
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==Reference==
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Flexible DNA target site recognition by divergent homing endonuclease isoschizomers I-CreI and I-MsoI., Chevalier B, Turmel M, Lemieux C, Monnat RJ Jr, Stoddard BL, J Mol Biol. 2003 May 30;329(2):253-69. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12758074 12758074]
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[[Category: Chlamydomonas reinhardtii]]
[[Category: Chlamydomonas reinhardtii]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Chevalier, B.]]
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[[Category: Chevalier B]]
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[[Category: Lemieux, C.]]
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[[Category: Lemieux C]]
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[[Category: Monnat, R J.]]
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[[Category: Monnat RJ]]
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[[Category: Stoddard, B L.]]
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[[Category: Stoddard BL]]
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[[Category: Turmel, M.]]
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[[Category: Turmel M]]
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[[Category: Dna recognition]]
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[[Category: Endonuclease]]
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[[Category: Homing]]
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[[Category: Laglidadg]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 18:19:29 2008''
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Current revision

Crystal structure of I-CreI bound to a palindromic DNA sequence I (palindrome of left side of wildtype DNA target sequence)

PDB ID 1n3e

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Proteopedia Page Contributors and Editors (what is this?)

OCA

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