We apologize for Proteopedia being slow to respond. For the past two years, a new implementation of Proteopedia has been being built. Soon, it will replace this 18-year old system. All existing content will be moved to the new system at a date that will be announced here.

5acn

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
(New page: 200px<br /><applet load="5acn" size="450" color="white" frame="true" align="right" spinBox="true" caption="5acn, resolution 2.1&Aring;" /> '''STRUCTURE OF ACTIVATE...)
Current revision (03:50, 21 November 2024) (edit) (undo)
 
(15 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:5acn.gif|left|200px]]<br /><applet load="5acn" size="450" color="white" frame="true" align="right" spinBox="true"
 
-
caption="5acn, resolution 2.1&Aring;" />
 
-
'''STRUCTURE OF ACTIVATED ACONITASE. FORMATION OF THE (4FE-4S) CLUSTER IN THE CRYSTAL'''<br />
 
-
==Overview==
+
==STRUCTURE OF ACTIVATED ACONITASE. FORMATION OF THE (4FE-4S) CLUSTER IN THE CRYSTAL==
-
The structure of activated pig heart aconitase [citrate(isocitrate), hydro-lyase, EC 4.2.1.3] containing a [4Fe-4S] cluster has been refined at, 2.5-A resolution to a crystallographic residual of 18.2%. Comparison of, this structure to the recently determined 2.1-A resolution structure of, the inactive enzyme containing a [3Fe-4S] cluster, by difference Fourier, analysis, shows that upon activation iron is inserted into the structure, isomorphously. The common atoms of the [3Fe-4S] and [4Fe-4S] cores agree, within 0.1 A; the three common cysteinyl S gamma ligand atoms agree within, 0.25 A. The fourth ligand of the Fe inserted into the [3Fe-4S] cluster is, a water or hydroxyl from solvent, consistent with the absence of a free, cysteine ligand in the enzyme active site cleft and the isomorphism of the, two structures. A water molecule occupies a similar site in the crystal, structure of the inactive enzyme.
+
<StructureSection load='5acn' size='340' side='right'caption='[[5acn]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
 +
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[5acn]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ACN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5ACN FirstGlance]. <br>
 +
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
 +
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=TRC:TRICARBALLYLIC+ACID'>TRC</scene></td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5acn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5acn OCA], [https://pdbe.org/5acn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5acn RCSB], [https://www.ebi.ac.uk/pdbsum/5acn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5acn ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/ACON_PIG ACON_PIG] Catalyzes the isomerization of citrate to isocitrate via cis-aconitate.
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ac/5acn_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=5acn ConSurf].
 +
<div style="clear:both"></div>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
The structure of activated pig heart aconitase [citrate(isocitrate) hydro-lyase, EC 4.2.1.3] containing a [4Fe-4S] cluster has been refined at 2.5-A resolution to a crystallographic residual of 18.2%. Comparison of this structure to the recently determined 2.1-A resolution structure of the inactive enzyme containing a [3Fe-4S] cluster, by difference Fourier analysis, shows that upon activation iron is inserted into the structure isomorphously. The common atoms of the [3Fe-4S] and [4Fe-4S] cores agree within 0.1 A; the three common cysteinyl S gamma ligand atoms agree within 0.25 A. The fourth ligand of the Fe inserted into the [3Fe-4S] cluster is a water or hydroxyl from solvent, consistent with the absence of a free cysteine ligand in the enzyme active site cleft and the isomorphism of the two structures. A water molecule occupies a similar site in the crystal structure of the inactive enzyme.
-
==About this Structure==
+
Structure of activated aconitase: formation of the [4Fe-4S] cluster in the crystal.,Robbins AH, Stout CD Proc Natl Acad Sci U S A. 1989 May;86(10):3639-43. PMID:2726740<ref>PMID:2726740</ref>
-
5ACN is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa] with SO4, F3S and TRC as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Aconitate_hydratase Aconitate hydratase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.3 4.2.1.3] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=5ACN OCA].
+
-
==Reference==
+
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-
Structure of activated aconitase: formation of the [4Fe-4S] cluster in the crystal., Robbins AH, Stout CD, Proc Natl Acad Sci U S A. 1989 May;86(10):3639-43. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=2726740 2726740]
+
</div>
-
[[Category: Aconitate hydratase]]
+
<div class="pdbe-citations 5acn" style="background-color:#fffaf0;"></div>
-
[[Category: Single protein]]
+
-
[[Category: Sus scrofa]]
+
-
[[Category: Robbins, A.H.]]
+
-
[[Category: Stout, C.D.]]
+
-
[[Category: F3S]]
+
-
[[Category: SO4]]
+
-
[[Category: TRC]]
+
-
[[Category: lyase(carbon-oxygen)]]
+
-
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 19:23:22 2007''
+
==See Also==
 +
*[[Aconitase 3D structures|Aconitase 3D structures]]
 +
== References ==
 +
<references/>
 +
__TOC__
 +
</StructureSection>
 +
[[Category: Large Structures]]
 +
[[Category: Sus scrofa]]
 +
[[Category: Robbins AH]]
 +
[[Category: Stout CD]]

Current revision

STRUCTURE OF ACTIVATED ACONITASE. FORMATION OF THE (4FE-4S) CLUSTER IN THE CRYSTAL

PDB ID 5acn

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5acn"
Personal tools