1knb

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(New page: 200px<br /><applet load="1knb" size="450" color="white" frame="true" align="right" spinBox="true" caption="1knb, resolution 1.7&Aring;" /> '''CRYSTAL STRUCTURE OF ...)
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[[Image:1knb.gif|left|200px]]<br /><applet load="1knb" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1knb, resolution 1.7&Aring;" />
 
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'''CRYSTAL STRUCTURE OF THE RECEPTOR-BINDING DOMAIN OF ADENOVIRUS TYPE 5 FIBER PROTEIN AT 1.7 ANGSTROMS RESOLUTION'''<br />
 
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==Overview==
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==CRYSTAL STRUCTURE OF THE RECEPTOR-BINDING DOMAIN OF ADENOVIRUS TYPE 5 FIBER PROTEIN AT 1.7 ANGSTROMS RESOLUTION==
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BACKGROUND: Adenoviral infection begins with the binding of virion to the, surface of host cells. Specific attachment is achieved through, interactions between host-cell receptors and the adenovirus fiber protein, and is mediated by the globular carboxy-terminal domain of the adenovirus, fiber protein, termed the carboxy-terminal knob domain. RESULTS: The, crystal structure of the carboxy-terminal knob domain of the adenovirus, type 5 (Ad5) fiber protein has been determined at 1.7 A resolution. Each, knob monomer forms an eight-stranded antiparallel beta-sandwich structure., In the crystal lattice, the knob monomers form closely interacting trimers, which possess a deep surface depression centered around the three-fold, molecular symmetry axis and three symmetry-related valleys. CONCLUSIONS:, The amino acid residues lining the wall of the central surface depression, and the three symmetry-related floors of the valleys are strictly, conserved in the knob domains of Ad5 and adenovirus type 2 (Ad2) fiber, proteins, which share the same cellular receptor. The beta-sandwich, structure of the knob monomer demonstrates a unique folding topology which, is different from that of other known antiparallel beta-sandwich, structures. The large buried surface area and numerous polar interactions, in the trimer indicate that this form of the knob protein is predominant, in solution, suggesting a possible assembly pathway for the native fiber, protein.
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<StructureSection load='1knb' size='340' side='right'caption='[[1knb]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1knb]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Human_adenovirus_5 Human adenovirus 5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KNB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1KNB FirstGlance]. <br>
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1KNB is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Human_adenovirus_11 Human adenovirus 11]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1KNB OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1knb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1knb OCA], [https://pdbe.org/1knb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1knb RCSB], [https://www.ebi.ac.uk/pdbsum/1knb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1knb ProSAT]</span></td></tr>
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==Reference==
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</table>
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Crystal structure of the receptor-binding domain of adenovirus type 5 fiber protein at 1.7 A resolution., Xia D, Henry LJ, Gerard RD, Deisenhofer J, Structure. 1994 Dec 15;2(12):1259-70. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=7704534 7704534]
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== Function ==
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[[Category: Human adenovirus 11]]
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[https://www.uniprot.org/uniprot/SPIKE_ADE05 SPIKE_ADE05] Forms spikes that protrude from each vertex of the icosahedral capsid. Interacts with host coxsackievirus and adenovirus receptor CXADR located at the cell tight junctions to provide virion initial attachment to target cell. The fiber protein binds to CXADR with a higher affinity than CXADR binds to itself, thereby blocking the cell-cell adhesion function of CXADR dimers and leading to local disruption of the tight junction. Fiber protein present on neo-synthesized particles may thus disrupt the junctional integrity in order to facilitate further neighboring cells infection. Fiber proteins are shed during virus entry, when virus is still at the cell surface. Fiber shedding is dependent on viral CXADR drifting motion and subsequent binding to immobile integrins. Heparan sulfate might also play a role in virus binding (By similarity).
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[[Category: Single protein]]
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== Evolutionary Conservation ==
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[[Category: Deisenhofer, J.]]
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[[Image:Consurf_key_small.gif|200px|right]]
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[[Category: Gerard, R.D.]]
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Check<jmol>
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[[Category: Henry, L.J.]]
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<jmolCheckbox>
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[[Category: Xia, D.]]
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/kn/1knb_consurf.spt"</scriptWhenChecked>
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[[Category: cell receptor recognition]]
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 19:23:58 2007''
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1knb ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
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[[Category: Human adenovirus 5]]
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[[Category: Large Structures]]
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[[Category: Deisenhofer J]]
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[[Category: Gerard RD]]
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[[Category: Henry LJ]]
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[[Category: Xia D]]

Current revision

CRYSTAL STRUCTURE OF THE RECEPTOR-BINDING DOMAIN OF ADENOVIRUS TYPE 5 FIBER PROTEIN AT 1.7 ANGSTROMS RESOLUTION

PDB ID 1knb

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