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2xim

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(New page: 200px<br /><applet load="2xim" size="450" color="white" frame="true" align="right" spinBox="true" caption="2xim, resolution 2.3&Aring;" /> '''ARGININE RESIDUES AS ...)
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[[Image:2xim.jpg|left|200px]]<br /><applet load="2xim" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="2xim, resolution 2.3&Aring;" />
 
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'''ARGININE RESIDUES AS STABILIZING ELEMENTS IN PROTEINS'''<br />
 
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==Overview==
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==ARGININE RESIDUES AS STABILIZING ELEMENTS IN PROTEINS==
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Site-specific substitutions of arginine for lysine in the thermostable, D-xylose isomerase (XI) from Actinoplanes missouriensis are shown to, impart significant heat stability enhancement in the presence of sugar, substrates most probably by interfering with nonenzymatic glycation. The, same substitutions are also found to increase heat stability in the, absence of any sugar derivatives, where a mechanism based on prevention of, glycation can no longer be invoked. This rather conservative substitution, is moreover shown to improve thermostability in two other structurally, unrelated proteins, human copper, zinc-superoxide dismutase (CuZnSOD) and, D-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) from Bacillus subtilis., The stabilizing effect of Lys----Arg substitutions is rationalized on the, basis of a detailed analysis of the crystal structures of wild-type XI and, of engineered variants with Lys----Arg substitution at four distinct, locations, residues 253, 309, 319, and 323. Molecular model building, analysis of the structures of wild-type and mutant CuZnSOD (K9R) and GAPDH, (G281K and G281R) is used to explain the observed stability enhancement in, these proteins. In addition to demonstrating that even thermostable, proteins can lend themselves to further stability improvement, our, findings provide direct evidence that arginine residues are important, stabilizing elements in proteins. Moreover, the stabilizing role of, electrostatic interactions, particularly between subunits in oligomeric, proteins, is documented.
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<StructureSection load='2xim' size='340' side='right'caption='[[2xim]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2xim]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Actinoplanes_missouriensis Actinoplanes missouriensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XIM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2XIM FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=XYL:D-XYLITOL'>XYL</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2xim FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xim OCA], [https://pdbe.org/2xim PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2xim RCSB], [https://www.ebi.ac.uk/pdbsum/2xim PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2xim ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/XYLA_ACTM4 XYLA_ACTM4]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/xi/2xim_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2xim ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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2XIM is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Actinoplanes_missouriensis Actinoplanes missouriensis] with XYL and MG as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Xylose_isomerase Xylose isomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.1.5 5.3.1.5] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2XIM OCA].
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*[[D-xylose isomerase 3D structures|D-xylose isomerase 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Arginine residues as stabilizing elements in proteins., Mrabet NT, Van den Broeck A, Van den brande I, Stanssens P, Laroche Y, Lambeir AM, Matthijssens G, Jenkins J, Chiadmi M, van Tilbeurgh H, et al., Biochemistry. 1992 Mar 3;31(8):2239-53. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=1540579 1540579]
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[[Category: Actinoplanes missouriensis]]
[[Category: Actinoplanes missouriensis]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Xylose isomerase]]
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[[Category: Chiadmi M]]
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[[Category: Brande, I.Van.Den.]]
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[[Category: Demaeyer M]]
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[[Category: Chiadmi, M.]]
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[[Category: Janin J]]
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[[Category: Demaeyer, M.]]
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[[Category: Jenkins J]]
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[[Category: Denbroek, A.Van.]]
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[[Category: Lambeir A-M]]
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[[Category: Janin, J.]]
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[[Category: Laroche Y]]
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[[Category: Jenkins, J.]]
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[[Category: Lasters I]]
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[[Category: Lambeir, A.M.]]
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[[Category: Matthyssens G]]
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[[Category: Laroche, Y.]]
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[[Category: Mrabet NT]]
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[[Category: Lasters, I.]]
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[[Category: Quax WJ]]
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[[Category: Matthyssens, G.]]
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[[Category: Rey F]]
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[[Category: Mrabet, N.T.]]
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[[Category: Stanssens P]]
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[[Category: Quax, W.J.]]
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[[Category: Van Den Brande I]]
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[[Category: Rey, F.]]
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[[Category: Van Denbroek A]]
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[[Category: Stanssens, P.]]
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[[Category: Vantilbeurgh H]]
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[[Category: Vantilbeurgh, H.]]
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[[Category: Wodak SJ]]
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[[Category: Wodak, S.J.]]
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[[Category: MG]]
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[[Category: XYL]]
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[[Category: isomerase(intramolecular oxidoreductse)]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 19:30:14 2007''
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Current revision

ARGININE RESIDUES AS STABILIZING ELEMENTS IN PROTEINS

PDB ID 2xim

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