4fis

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(New page: 200px<br /><applet load="4fis" size="450" color="white" frame="true" align="right" spinBox="true" caption="4fis, resolution 2.3&Aring;" /> '''THE MOLECULAR STRUCTU...)
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[[Image:4fis.jpg|left|200px]]<br /><applet load="4fis" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="4fis, resolution 2.3&Aring;" />
 
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'''THE MOLECULAR STRUCTURE OF WILD-TYPE AND A MUTANT FIS PROTEIN: RELATIONSHIP BETWEEN MUTATIONAL CHANGES AND RECOMBINATIONAL ENHANCER FUNCTION OR DNA BINDING'''<br />
 
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==Overview==
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==THE MOLECULAR STRUCTURE OF WILD-TYPE AND A MUTANT FIS PROTEIN: RELATIONSHIP BETWEEN MUTATIONAL CHANGES AND RECOMBINATIONAL ENHANCER FUNCTION OR DNA BINDING==
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The 98-amino acid Fis protein from Escherichia coli functions in a variety, of reactions, including promotion of Hin-mediated site-specific DNA, inversion when bound to an enhancer sequence. It is unique among, site-specific DNA-binding proteins in that it binds to a large number of, different DNA sequences, for which a consensus sequence is difficult to, establish. X-ray crystal structure analyses have been carried out at 2.3 A, resolution for wild-type Fis and for an Arg-89----Cys mutant that does not, stimulate DNA inversion. Each monomer of the Fis dimer has four, alpha-helices, A-D; the first 19 residues are disordered in the crystal., The end of each C helix is hydrogen bonded to the beginning of helix B', from the opposite subunit in what effectively is one long continuous, although bent, helix. The four helices, C, B', C', and B, together define, a platform through the center of the Fis molecule: helices A and A' are, believed to be involved with Hin recombinase on one side, and helices D, and D' interact with DNA lying on the other side of the platform. Helices, C and D of each subunit comprise a helix-turn-helix (HTH) DNA-binding, element. The spacing of these two HTH elements in the dimer, 25 A, is too, short to allow insertion into adjacent major grooves of a straight B-DNA, helix. However, bending the DNA at discrete points, to an overall radius, of curvature of 62 A, allows efficient docking of a B-DNA helix with the, Fis molecule. The proposed complex explains the experimentally observed, patterns of methylation protection and DNase I cleavage hypersensitivity., The x-ray structure accounts for the effects of mutations in the Fis, sequence. Those that affect DNA inversion but not DNA binding are located, within the N-terminal disordered region and helix A. This inversion, activation domain is physically separated in the Fis molecule from the HTH, elements and may specify a region of contact with the Hin recombinase. In, contrast, mutations that affect HTH helices C and D, or interactions of, these with helix B, have the additional effect of decreasing or, eliminating binding to DNA.
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<StructureSection load='4fis' size='340' side='right'caption='[[4fis]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[4fis]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FIS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4FIS FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4fis FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4fis OCA], [https://pdbe.org/4fis PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4fis RCSB], [https://www.ebi.ac.uk/pdbsum/4fis PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4fis ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/FIS_ECOLI FIS_ECOLI] Activates ribosomal RNA transcription, as well other genes. Plays a direct role in upstream activation of rRNA promoters. Binds to a recombinational enhancer sequence that is required to stimulate hin-mediated DNA inversion. Prevents initiation of DNA replication from oriC.<ref>PMID:2209559</ref> <ref>PMID:8836178</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fi/4fis_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=4fis ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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4FIS is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=4FIS OCA].
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*[[FIS protein|FIS protein]]
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== References ==
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==Reference==
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<references/>
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The molecular structure of wild-type and a mutant Fis protein: relationship between mutational changes and recombinational enhancer function or DNA binding., Yuan HS, Finkel SE, Feng JA, Kaczor-Grzeskowiak M, Johnson RC, Dickerson RE, Proc Natl Acad Sci U S A. 1991 Nov 1;88(21):9558-62. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=1946369 1946369]
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__TOC__
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</StructureSection>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Dickerson, R.E.]]
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[[Category: Dickerson RE]]
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[[Category: Feng, J.A.]]
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[[Category: Feng J-A]]
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[[Category: Finkel, S.E.]]
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[[Category: Finkel SE]]
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[[Category: Johnson, R.C.]]
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[[Category: Johnson RC]]
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[[Category: Yuan, H.S.]]
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[[Category: Yuan HS]]
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[[Category: dna-binding protein]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 19:35:29 2007''
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Current revision

THE MOLECULAR STRUCTURE OF WILD-TYPE AND A MUTANT FIS PROTEIN: RELATIONSHIP BETWEEN MUTATIONAL CHANGES AND RECOMBINATIONAL ENHANCER FUNCTION OR DNA BINDING

PDB ID 4fis

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