1umn

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{{Seed}}
 
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[[Image:1umn.png|left|200px]]
 
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==Crystal structure of Dps-like peroxide resistance protein (Dpr) from Streptococcus suis==
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The line below this paragraph, containing "STRUCTURE_1umn", creates the "Structure Box" on the page.
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<StructureSection load='1umn' size='340' side='right'caption='[[1umn]], [[Resolution|resolution]] 1.95&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1umn]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_suis Streptococcus suis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UMN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1UMN FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.95&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene></td></tr>
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{{STRUCTURE_1umn| PDB=1umn | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1umn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1umn OCA], [https://pdbe.org/1umn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1umn RCSB], [https://www.ebi.ac.uk/pdbsum/1umn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1umn ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/DPS_STRSU DPS_STRSU] Protects DNA from oxidative damage by sequestering intracellular Fe(2+) ion and storing it in the form of Fe(3+) oxyhydroxide mineral. One hydrogen peroxide oxidizes two Fe(2+) ions, which prevents hydroxyl radical production by the Fenton reaction (By similarity). It binds and incorporates Fe(2+) iron. Is responsible for hydrogen peroxide resistance. Does not bind DNA.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/um/1umn_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1umn ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The Dps-like peroxide resistance protein (Dpr) is an aerotolerance and hydrogen peroxide resistance agent found in the meningitis-associated pathogen Streptococcus suis. Dpr is believed to act by binding free intracellular iron to prevent Fenton chemistry-catalysed formation of toxic hydroxyl radicals. The crystal structure of Dpr has been determined to 1.95 A resolution. The final model has an Rcyst value of 18.5% (Rfree = 22.4%) and consists of 12 identical monomers (each of them comprising a four alpha-helix bundle) that form a hollow sphere obeying 23 symmetry. Structural features show that Dpr belongs to the Dps family of bacterial proteins. Twelve putative ferroxidase centers, each formed at the interface of neighboring monomer pairs, were identified in the Dpr structure with structural similarities to those found in other Dps family members. Dpr was crystallized in the absence of iron, hence no bound iron was found in the structure in contrast to other Dps family members. A novel metal-binding site approximately 6A from the ferroxidase centre was identified and assigned to a bound calcium ion. Two residues from the ferroxidase centre (Asp63 and Asp74) were found to be involved in calcium binding. Structural comparison with other family members revealed that Asp63 and Asp74 adopt different conformation in the Dpr structure. The structure of Dpr presented here shows potential local conformational changes that may occur during iron incorporation. A role for the metal-binding site in iron uptake is proposed.
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===CRYSTAL STRUCTURE OF DPS-LIKE PEROXIDE RESISTANCE PROTEIN (DPR) FROM STREPTOCOCCUS SUIS===
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Crystal structure of Streptococcus suis Dps-like peroxide resistance protein Dpr: implications for iron incorporation.,Kauko A, Haataja S, Pulliainen AT, Finne J, Papageorgiou AC J Mol Biol. 2004 Apr 30;338(3):547-58. PMID:15081812<ref>PMID:15081812</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_15081812}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 1umn" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 15081812 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_15081812}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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1UMN is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Streptococcus_suis Streptococcus suis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UMN OCA].
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==Reference==
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Crystal structure of Streptococcus suis Dps-like peroxide resistance protein Dpr: implications for iron incorporation., Kauko A, Haataja S, Pulliainen AT, Finne J, Papageorgiou AC, J Mol Biol. 2004 Apr 30;338(3):547-58. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15081812 15081812]
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[[Category: Single protein]]
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[[Category: Streptococcus suis]]
[[Category: Streptococcus suis]]
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[[Category: Finne, J.]]
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[[Category: Finne J]]
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[[Category: Haataja, S.]]
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[[Category: Haataja S]]
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[[Category: Kauko, A.]]
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[[Category: Kauko A]]
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[[Category: Papageorgiou, A C.]]
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[[Category: Papageorgiou AC]]
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[[Category: Pulliainen, A.]]
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[[Category: Pulliainen A]]
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[[Category: Dps-family]]
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[[Category: Ferritin-like]]
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[[Category: Ferroxidase]]
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[[Category: Iron-binding]]
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[[Category: Peroxide resistance]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 20:15:16 2008''
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Current revision

Crystal structure of Dps-like peroxide resistance protein (Dpr) from Streptococcus suis

PDB ID 1umn

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