3cox

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(New page: 200px<br /><applet load="3cox" size="450" color="white" frame="true" align="right" spinBox="true" caption="3cox, resolution 1.8&Aring;" /> '''CRYSTAL STRUCTURE OF ...)
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[[Image:3cox.gif|left|200px]]<br /><applet load="3cox" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="3cox, resolution 1.8&Aring;" />
 
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'''CRYSTAL STRUCTURE OF CHOLESTEROL OXIDASE COMPLEXED WITH A STEROID SUBSTRATE. IMPLICATIONS FOR FAD DEPENDENT ALCOHOL OXIDASES'''<br />
 
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==Overview==
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==CRYSTAL STRUCTURE OF CHOLESTEROL OXIDASE COMPLEXED WITH A STEROID SUBSTRATE. IMPLICATIONS FOR FAD DEPENDENT ALCOHOL OXIDASES==
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Cholesterol oxidase from Brevibacterium sterolicum is a flavin-dependent, enzyme that catalyzes the oxidation and isomerization of 3 beta-hydroxy, steroids with a double bond at delta 5-delta 6 of the steroid ring, backbone. The crystal structure of the free enzyme in the absence of a, steroid substrate has previously been determined. In this paper we report, the crystal structure of the complex of cholesterol oxidase with the, steroid substrate dehydroisoandrosterone, refined at 1.8-A resolution. The, final crystallographic R-value is 15.7% for all reflections between 10.0-, and 1.8-A resolution. The steroid is buried within the protein in an, internal cavity which, in the free enzyme crystal structure, was occupied, by a lattice of water molecules. The conformations of a number of side, chains lining the active-site cavity have changed in order to accommodate, the steroid substrate. A loop region of the structure between residues 70, and 90 differs significantly between the substrate-free and, substrate-bound forms of the enzyme, presumably to facilitate binding of, the steroid. The hydroxyl group of the steroid substrate is, hydrogen-bonded to both the flavin ring system of the FAD cofactor and a, bound water molecule. FAD-dependent cholesterol oxidase shares significant, structural homology with another flavoenzyme, glucose oxidase, suggesting, that it might also be a member of the glucose-methanol-choline (GMC), oxidoreductase family. Although there is only limited sequence homology, a, superposition of these two structures reveals a conserved histidine, residue within hydrogen-bonding distance of the active-site water, molecule.(ABSTRACT TRUNCATED AT 250 WORDS)
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<StructureSection load='3cox' size='340' side='right'caption='[[3cox]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3cox]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Brevibacterium_sterolicum Brevibacterium sterolicum]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1cox 1cox]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3COX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3COX FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3cox FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3cox OCA], [https://pdbe.org/3cox PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3cox RCSB], [https://www.ebi.ac.uk/pdbsum/3cox PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3cox ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/CHOD_BREST CHOD_BREST] Catalyzes the oxidation and isomerization of cholesterol to cholestenone (4-cholesten-3-one), which is an initial step in the cholesterol degradation process.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/co/3cox_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3cox ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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3COX is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Brevibacterium_sterolicum Brevibacterium sterolicum] with FAD as [http://en.wikipedia.org/wiki/ligand ligand]. This structure superseeds the now removed PDB entry 1COX. Active as [http://en.wikipedia.org/wiki/Cholesterol_oxidase Cholesterol oxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.3.6 1.1.3.6] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=3COX OCA].
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*[[Cholesterol oxidase|Cholesterol oxidase]]
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__TOC__
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==Reference==
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</StructureSection>
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Crystal structure of cholesterol oxidase complexed with a steroid substrate: implications for flavin adenine dinucleotide dependent alcohol oxidases., Li J, Vrielink A, Brick P, Blow DM, Biochemistry. 1993 Nov 2;32(43):11507-15. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8218217 8218217]
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[[Category: Brevibacterium sterolicum]]
[[Category: Brevibacterium sterolicum]]
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[[Category: Cholesterol oxidase]]
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[[Category: Large Structures]]
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[[Category: Single protein]]
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[[Category: Blow DM]]
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[[Category: Blow, D.M.]]
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[[Category: Brick P]]
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[[Category: Brick, P.]]
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[[Category: Li J]]
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[[Category: Li, J.]]
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[[Category: Vrielink A]]
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[[Category: Vrielink, A.]]
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[[Category: FAD]]
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[[Category: oxidoreductase(oxygen receptor)]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 19:36:13 2007''
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Current revision

CRYSTAL STRUCTURE OF CHOLESTEROL OXIDASE COMPLEXED WITH A STEROID SUBSTRATE. IMPLICATIONS FOR FAD DEPENDENT ALCOHOL OXIDASES

PDB ID 3cox

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