1ukc

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{{Seed}}
 
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[[Image:1ukc.png|left|200px]]
 
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==Crystal Structure of Aspergillus niger EstA==
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The line below this paragraph, containing "STRUCTURE_1ukc", creates the "Structure Box" on the page.
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<StructureSection load='1ukc' size='340' side='right'caption='[[1ukc]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1ukc]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Aspergillus_niger Aspergillus niger]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UKC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1UKC FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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{{STRUCTURE_1ukc| PDB=1ukc | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ukc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ukc OCA], [https://pdbe.org/1ukc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ukc RCSB], [https://www.ebi.ac.uk/pdbsum/1ukc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ukc ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q6ED33_ASPNG Q6ED33_ASPNG]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/uk/1ukc_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ukc ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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From the fungus Aspergillus niger, we identified a new gene encoding protein EstA, a member of the alpha/beta-hydrolase fold superfamily but of unknown substrate specificity. EstA was overexpressed and its crystal structure was solved by molecular replacement using a lipase-acetylcholinesterase chimera template. The 2.1 A resolution structure of EstA reveals a canonical Ser/Glu/His catalytic triad located in a small pocket at the bottom of a large solvent-accessible, bowl-shaped cavity. Potential substrates selected by manual docking procedures were assayed for EstA activity. Consistent with the pocket geometry, preference for hydrolysis of short acyl/propyl chain substrates was found. Identification of close homologs from the genome of other fungi, of which some are broad host-range pathogens, defines EstA as the first member of a novel class of fungal esterases within the superfamily. Hence the structure of EstA constitutes a lead template in the design of new antifungal agents directed toward its pathogenic homologs.
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===Crystal Structure of Aspergillus niger EstA===
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Aspergillus niger protein EstA defines a new class of fungal esterases within the alpha/beta hydrolase fold superfamily of proteins.,Bourne Y, Hasper AA, Chahinian H, Juin M, De Graaff LH, Marchot P Structure. 2004 Apr;12(4):677-87. PMID:15062090<ref>PMID:15062090</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_15062090}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 1ukc" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 15062090 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_15062090}}
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__TOC__
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</StructureSection>
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==About this Structure==
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1UKC is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Aspergillus_niger Aspergillus niger]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UKC OCA].
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==Reference==
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Aspergillus niger protein EstA defines a new class of fungal esterases within the alpha/beta hydrolase fold superfamily of proteins., Bourne Y, Hasper AA, Chahinian H, Juin M, De Graaff LH, Marchot P, Structure. 2004 Apr;12(4):677-87. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15062090 15062090]
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[[Category: Aspergillus niger]]
[[Category: Aspergillus niger]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Bourne, Y.]]
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[[Category: Bourne Y]]
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[[Category: Chahinian, H.]]
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[[Category: Chahinian H]]
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[[Category: Graaff, L H.De.]]
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[[Category: De Graaff LH]]
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[[Category: Hasper, A A.]]
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[[Category: Hasper AA]]
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[[Category: Juin, M.]]
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[[Category: Juin M]]
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[[Category: Marchot, P.]]
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[[Category: Marchot P]]
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[[Category: A/b hydrolase fold]]
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[[Category: Acetylcholinesterase]]
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[[Category: Esterase]]
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[[Category: Fungi]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 20:18:39 2008''
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Current revision

Crystal Structure of Aspergillus niger EstA

PDB ID 1ukc

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OCA

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