3cro

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(New page: 200px<br /><applet load="3cro" size="450" color="white" frame="true" align="right" spinBox="true" caption="3cro, resolution 2.500&Aring;" /> '''THE PHAGE 434 CRO/O...)
Current revision (09:37, 21 February 2024) (edit) (undo)
 
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[[Image:3cro.jpg|left|200px]]<br /><applet load="3cro" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="3cro, resolution 2.500&Aring;" />
 
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'''THE PHAGE 434 CRO/OR1 COMPLEX AT 2.5 ANGSTROMS RESOLUTION'''<br />
 
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==Overview==
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==THE PHAGE 434 CRO/OR1 COMPLEX AT 2.5 ANGSTROMS RESOLUTION==
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The crystal structure of phage 434 Cro protein in complex with a 20, base-pair DNA fragment has been determined to 2.5 A resolution. The DNA, fragment contains the sequence of the OR1 operator site. The structure, shows a bent conformation for the DNA, straighter at the center and more, bent at the ends. The central base-pairs adopt conformations with, significant deviations from coplanarity. The two molecules interact, extensively along their common interface, both through hydrogen bonds and, van der Waals interactions. The significance of these interactions for, operator binding and recognition is discussed.
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<StructureSection load='3cro' size='340' side='right'caption='[[3cro]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[3cro]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Phage_434 Phage 434]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CRO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3CRO FirstGlance]. <br>
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3CRO is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacteriophage_434 Bacteriophage 434]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=3CRO OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3cro FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3cro OCA], [https://pdbe.org/3cro PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3cro RCSB], [https://www.ebi.ac.uk/pdbsum/3cro PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3cro ProSAT]</span></td></tr>
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==Reference==
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</table>
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The phage 434 Cro/OR1 complex at 2.5 A resolution., Mondragon A, Harrison SC, J Mol Biol. 1991 May 20;219(2):321-34. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=2038059 2038059]
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== Function ==
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[[Category: Bacteriophage 434]]
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[https://www.uniprot.org/uniprot/RCRO_BP434 RCRO_BP434] Cro represses genes normally expressed in early phage development and is necessary for the late stage of lytic growth. It does this by binding to the OL and OR operators regions normally used by the repressor protein for lysogenic maintenance.
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[[Category: Single protein]]
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== Evolutionary Conservation ==
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[[Category: Harrison, S.C.]]
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[[Image:Consurf_key_small.gif|200px|right]]
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[[Category: Mondragon, A.]]
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Check<jmol>
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[[Category: double helix]]
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<jmolCheckbox>
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[[Category: protein-dna complex]]
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cr/3cro_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 19:37:06 2007''
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3cro ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
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[[Category: Phage 434]]
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[[Category: Harrison SC]]
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[[Category: Mondragon A]]

Current revision

THE PHAGE 434 CRO/OR1 COMPLEX AT 2.5 ANGSTROMS RESOLUTION

PDB ID 3cro

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