1sh3

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{{Seed}}
 
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[[Image:1sh3.png|left|200px]]
 
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==Crystal Structure of Norwalk Virus Polymerase (MgSO4 crystal form)==
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The line below this paragraph, containing "STRUCTURE_1sh3", creates the "Structure Box" on the page.
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<StructureSection load='1sh3' size='340' side='right'caption='[[1sh3]], [[Resolution|resolution]] 2.95&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1sh3]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Norwalk_virus Norwalk virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SH3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1SH3 FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.95&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
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{{STRUCTURE_1sh3| PDB=1sh3 | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1sh3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1sh3 OCA], [https://pdbe.org/1sh3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1sh3 RCSB], [https://www.ebi.ac.uk/pdbsum/1sh3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1sh3 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q70ET3_9CALI Q70ET3_9CALI]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/sh/1sh3_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1sh3 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Norwalk virus is a major cause of acute gastroenteritis for which effective treatments are sorely lacking. To provide a basis for the rational design of novel antiviral agents, the main replication enzyme in Norwalk virus, the virally encoded RNA-dependent RNA polymerase (RdRP), has been expressed in an enzymatically active form, and its structure has been crystallographically determined both in the presence and absence of divalent metal cations. Although the overall fold of the enzyme is similar to that seen previously in the RdRP from rabbit hemorrhagic disease virus, the carboxyl terminus, surprisingly, is located in the active site cleft in five independent copies of the protein in three distinct crystal forms. The location of this carboxyl-terminal segment appears to interfere with the binding of double-stranded RNA in the active site cleft and may play a role in the initiation of RNA synthesis or mediate interactions with accessory replication proteins.
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===Crystal Structure of Norwalk Virus Polymerase (MgSO4 crystal form)===
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Crystal structure of norwalk virus polymerase reveals the carboxyl terminus in the active site cleft.,Ng KK, Pendas-Franco N, Rojo J, Boga JA, Machin A, Alonso JM, Parra F J Biol Chem. 2004 Apr 16;279(16):16638-45. Epub 2004 Feb 5. PMID:14764591<ref>PMID:14764591</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 1sh3" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_14764591}}, adds the Publication Abstract to the page
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*[[RNA polymerase 3D structures|RNA polymerase 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 14764591 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_14764591}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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1SH3 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Norwalk_virus Norwalk virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SH3 OCA].
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==Reference==
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Crystal structure of norwalk virus polymerase reveals the carboxyl terminus in the active site cleft., Ng KK, Pendas-Franco N, Rojo J, Boga JA, Machin A, Alonso JM, Parra F, J Biol Chem. 2004 Apr 16;279(16):16638-45. Epub 2004 Feb 5. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14764591 14764591]
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[[Category: Norwalk virus]]
[[Category: Norwalk virus]]
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[[Category: RNA-directed RNA polymerase]]
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[[Category: Alonso JM]]
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[[Category: Single protein]]
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[[Category: Boga JA]]
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[[Category: Alonso, J M.]]
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[[Category: Machin A]]
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[[Category: Boga, J A.]]
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[[Category: Ng KK]]
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[[Category: Machin, A.]]
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[[Category: Parra F]]
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[[Category: Ng, K K.]]
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[[Category: Pendas-Franco N]]
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[[Category: Parra, F.]]
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[[Category: Rojo J]]
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[[Category: Pendas-Franco, N.]]
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[[Category: Rojo, J.]]
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[[Category: Rna polymerase]]
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[[Category: Viral replication enzyme]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 20:33:10 2008''
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Current revision

Crystal Structure of Norwalk Virus Polymerase (MgSO4 crystal form)

PDB ID 1sh3

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