This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


4icb

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
(New page: 200px<br /><applet load="4icb" size="450" color="white" frame="true" align="right" spinBox="true" caption="4icb, resolution 1.6&Aring;" /> '''PROLINE CIS-TRANS ISO...)
Current revision (11:53, 1 March 2024) (edit) (undo)
 
(12 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:4icb.gif|left|200px]]<br /><applet load="4icb" size="450" color="white" frame="true" align="right" spinBox="true"
 
-
caption="4icb, resolution 1.6&Aring;" />
 
-
'''PROLINE CIS-TRANS ISOMERS IN CALBINDIN D9K OBSERVED BY X-RAY CRYSTALLOGRAPHY'''<br />
 
-
==Overview==
+
==PROLINE CIS-TRANS ISOMERS IN CALBINDIN D9K OBSERVED BY X-RAY CRYSTALLOGRAPHY==
-
In a structure of recombinant bovine calbindin D9k, determined, crystallographically to 1.6 A resolution, a proline in mixed, approximately equally populated, cis and trans conformation is observed., Isomers of this kind have not been reported in structure determinations of, calbindin D9k to 2.3 A resolution or in any other crystallographically, determined protein structure. The cis-trans isomerization occurs at the, peptide bond between Gly42 and Pro43, which is in agreement with results, from two-dimensional 1H nuclear magnetic resonance spectroscopy, experiments on solutions of calbindin D9k. Alternative backbone stretches, have been modeled and refined by stereochemical restrained least-squares, refinement for the segment Lys41 to Pro43. The final R-value was 0.188., The structural perturbations accompanying the cis-trans isomerization are, found to be very localized. The largest positional differences are, observed at residue Gly42, in which the alternative positions of the, oxygen atom are 3.6 A apart.
+
<StructureSection load='4icb' size='340' side='right'caption='[[4icb]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
 +
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[4icb]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ICB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4ICB FirstGlance]. <br>
 +
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6&#8491;</td></tr>
 +
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4icb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4icb OCA], [https://pdbe.org/4icb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4icb RCSB], [https://www.ebi.ac.uk/pdbsum/4icb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4icb ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/S100G_BOVIN S100G_BOVIN]
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ic/4icb_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=4icb ConSurf].
 +
<div style="clear:both"></div>
-
==About this Structure==
+
==See Also==
-
4ICB is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] with CA as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=4ICB OCA].
+
*[[S100 proteins 3D structures|S100 proteins 3D structures]]
-
 
+
__TOC__
-
==Reference==
+
</StructureSection>
-
Proline cis-trans isomers in calbindin D9k observed by X-ray crystallography., Svensson LA, Thulin E, Forsen S, J Mol Biol. 1992 Feb 5;223(3):601-6. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=1542107 1542107]
+
[[Category: Bos taurus]]
[[Category: Bos taurus]]
-
[[Category: Single protein]]
+
[[Category: Large Structures]]
-
[[Category: Svensson, L.A.]]
+
[[Category: Svensson LA]]
-
[[Category: CA]]
+
-
[[Category: calcium-binding protein]]
+
-
 
+
-
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 19:39:24 2007''
+

Current revision

PROLINE CIS-TRANS ISOMERS IN CALBINDIN D9K OBSERVED BY X-RAY CRYSTALLOGRAPHY

PDB ID 4icb

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4icb"
Personal tools