5tgl

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(New page: 200px<br /><applet load="5tgl" size="450" color="white" frame="true" align="right" spinBox="true" caption="5tgl, resolution 3.0&Aring;" /> '''A MODEL FOR INTERFACI...)
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[[Image:5tgl.gif|left|200px]]<br /><applet load="5tgl" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="5tgl, resolution 3.0&Aring;" />
 
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'''A MODEL FOR INTERFACIAL ACTIVATION IN LIPASES FROM THE STRUCTURE OF A FUNGAL LIPASE-INHIBITOR COMPLEX'''<br />
 
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==Overview==
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==A MODEL FOR INTERFACIAL ACTIVATION IN LIPASES FROM THE STRUCTURE OF A FUNGAL LIPASE-INHIBITOR COMPLEX==
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Lipases are hydrolytic enzymes which break down triacylglycerides into, free fatty acids and glycerols. They have been classified as serine, hydrolases owing to their inhibition by diethyl p-nitrophenyl phosphate., Lipase activity is greatly increased at the lipid-water interface, a, phenomenon known as interfacial activation. X-ray analysis has revealed, the atomic structures of two triacylglycerol lipases, unrelated in, sequence: the human pancreatic lipase (hPL)4, and an enzyme isolated from, the fungus Rhizomucor (formerly Mucor) miehei (RmL). In both enzymes the, active centres contain structurally analogous Asp-His-Ser triads, (characteristic of serine proteinases), which are buried completely, beneath a short helical segment, or 'lid'. Here we present the crystal, structure (at 3 A resolution) of a complex of R. miehei lipase with, n-hexylphosphonate ethyl ester in which the enzyme's active site is, exposed by the movement of the helical lid. This movement also increases, the nonpolarity of the surface surrounding the catalytic site. We propose, that the structure of the enzyme in this complex is equivalent to the, activated state generated by the oil-water interface.
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<StructureSection load='5tgl' size='340' side='right'caption='[[5tgl]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[5tgl]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Rhizomucor_miehei Rhizomucor miehei]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5TGL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5TGL FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HEE:N-HEXYLPHOSPHONATE+ETHYL+ESTER'>HEE</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5tgl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5tgl OCA], [https://pdbe.org/5tgl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5tgl RCSB], [https://www.ebi.ac.uk/pdbsum/5tgl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5tgl ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/LIP_RHIMI LIP_RHIMI]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/tg/5tgl_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=5tgl ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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5TGL is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Rhizomucor_miehei Rhizomucor miehei] with HEE as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Triacylglycerol_lipase Triacylglycerol lipase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.3 3.1.1.3] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=5TGL OCA].
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*[[Lipase 3D Structures|Lipase 3D Structures]]
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__TOC__
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==Reference==
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</StructureSection>
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A model for interfacial activation in lipases from the structure of a fungal lipase-inhibitor complex., Brzozowski AM, Derewenda U, Derewenda ZS, Dodson GG, Lawson DM, Turkenburg JP, Bjorkling F, Huge-Jensen B, Patkar SA, Thim L, Nature. 1991 Jun 6;351(6326):491-4. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=2046751 2046751]
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[[Category: Large Structures]]
[[Category: Rhizomucor miehei]]
[[Category: Rhizomucor miehei]]
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[[Category: Single protein]]
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[[Category: Bjorkling F]]
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[[Category: Triacylglycerol lipase]]
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[[Category: Brzozowski AM]]
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[[Category: Bjorkling, F.]]
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[[Category: Derewenda U]]
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[[Category: Brzozowski, A.M.]]
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[[Category: Derewenda ZS]]
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[[Category: Derewenda, U.]]
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[[Category: Dodson GG]]
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[[Category: Derewenda, Z.S.]]
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[[Category: Huge-Jensen B]]
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[[Category: Dodson, G.G.]]
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[[Category: Lawson D]]
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[[Category: Huge-Jensen, B.]]
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[[Category: Patkar SR]]
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[[Category: Lawson, D.]]
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[[Category: Thim L]]
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[[Category: Patkar, S.R.]]
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[[Category: Turkenburg JP]]
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[[Category: Thim, L.]]
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[[Category: Turkenburg, J.P.]]
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[[Category: HEE]]
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[[Category: hydrolase(carboxylic esterase)]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 19:42:16 2007''
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Current revision

A MODEL FOR INTERFACIAL ACTIVATION IN LIPASES FROM THE STRUCTURE OF A FUNGAL LIPASE-INHIBITOR COMPLEX

PDB ID 5tgl

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