1krh

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(New page: 200px<br /><applet load="1krh" size="450" color="white" frame="true" align="right" spinBox="true" caption="1krh, resolution 1.5&Aring;" /> '''X-ray Stucture of Ben...)
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[[Image:1krh.jpg|left|200px]]<br /><applet load="1krh" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1krh, resolution 1.5&Aring;" />
 
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'''X-ray Stucture of Benzoate Dioxygenase Reductase'''<br />
 
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==Overview==
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==X-ray Structure of Benzoate Dioxygenase Reductase==
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One of the major processes for aerobic biodegradation of aromatic, compounds is initiated by Rieske dioxygenases. Benzoate dioxygenase, contains a reductase component, BenC, that is responsible for the, two-electron transfer from NADH via FAD and an iron-sulfur cluster to the, terminal oxygenase component. Here, we present the structure of BenC from, Acinetobacter sp. strain ADP1 at 1.5 A resolution. BenC contains three, domains, each binding a redox cofactor: iron-sulfur, FAD and NADH, respectively. The [2Fe-2S] domain is similar to that of plant ferredoxins, and the FAD and NADH domains are similar to members of the, ferredoxin:NADPH reductase superfamily. In phthalate dioxygenase, reductase, the only other Rieske dioxygenase reductase for which a crystal, structure is available, the ferredoxin-like and flavin binding domains are, sequentially reversed compared to BenC. The BenC structure shows, significant differences in the location of the ferredoxin domain relative, to the other domains, compared to phthalate dioxygenase reductase and, other known systems containing these three domains. In BenC, the, ferredoxin domain interacts with both the flavin and NAD(P)H domains. The, iron-sulfur center and the flavin are about 9 A apart, which allows a fast, electron transfer. The BenC structure is the first determined for a, reductase from the class IB Rieske dioxygenases, whose reductases transfer, electrons directly to their oxygenase components. Based on sequence, similarities, a very similar structure was modeled for the class III, naphthalene dioxygenase reductase, which transfers electrons to an, intermediary ferredoxin, rather than the oxygenase component.
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<StructureSection load='1krh' size='340' side='right'caption='[[1krh]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1krh]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Acinetobacter_sp. Acinetobacter sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KRH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1KRH FirstGlance]. <br>
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1KRH is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Acinetobacter_sp. Acinetobacter sp.] with SO4, FES and FAD as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Ferredoxin--NAD(+)_reductase Ferredoxin--NAD(+) reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.18.1.3 1.18.1.3] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1KRH OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.5&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=FES:FE2/S2+(INORGANIC)+CLUSTER'>FES</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1krh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1krh OCA], [https://pdbe.org/1krh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1krh RCSB], [https://www.ebi.ac.uk/pdbsum/1krh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1krh ProSAT]</span></td></tr>
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X-ray crystal structure of benzoate 1,2-dioxygenase reductase from Acinetobacter sp. strain ADP1., Karlsson A, Beharry ZM, Matthew Eby D, Coulter ED, Neidle EL, Kurtz DM Jr, Eklund H, Ramaswamy S, J Mol Biol. 2002 Apr 26;318(2):261-72. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12051836 12051836]
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</table>
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[[Category: Acinetobacter sp.]]
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== Function ==
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[[Category: Ferredoxin--NAD(+) reductase]]
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[https://www.uniprot.org/uniprot/BENC_ACIAD BENC_ACIAD] Electron transfer component of benzoate 1,2-dioxygenase system.
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[[Category: Single protein]]
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== Evolutionary Conservation ==
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[[Category: Beharry, Z.M.]]
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[[Image:Consurf_key_small.gif|200px|right]]
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[[Category: Coulter, E.D.]]
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Check<jmol>
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[[Category: Eby, D.M.]]
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<jmolCheckbox>
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[[Category: Eklund, H.]]
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/kr/1krh_consurf.spt"</scriptWhenChecked>
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[[Category: Jr., D.M.Kurtz.]]
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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[[Category: Karlsson, A.]]
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<text>to colour the structure by Evolutionary Conservation</text>
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[[Category: Niedle, E.L.]]
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</jmolCheckbox>
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[[Category: Ramaswamy, S.]]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1krh ConSurf].
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[[Category: FAD]]
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<div style="clear:both"></div>
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[[Category: FES]]
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__TOC__
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[[Category: SO4]]
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</StructureSection>
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[[Category: alpha-beta]]
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[[Category: Acinetobacter sp]]
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[[Category: fad-binding]]
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[[Category: Large Structures]]
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[[Category: ferredoxin]]
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[[Category: Beharry ZM]]
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[[Category: nadh-binding]]
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[[Category: Coulter ED]]
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[[Category: Eby DM]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 19:44:49 2007''
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[[Category: Eklund H]]
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[[Category: Karlsson A]]
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[[Category: Kurtz Jr DM]]
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[[Category: Niedle EL]]
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[[Category: Ramaswamy S]]

Current revision

X-ray Structure of Benzoate Dioxygenase Reductase

PDB ID 1krh

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