3hts

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(New page: 200px<br /><applet load="3hts" size="450" color="white" frame="true" align="right" spinBox="true" caption="3hts, resolution 1.75&Aring;" /> '''HEAT SHOCK TRANSCRIP...)
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[[Image:3hts.jpg|left|200px]]<br /><applet load="3hts" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="3hts, resolution 1.75&Aring;" />
 
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'''HEAT SHOCK TRANSCRIPTION FACTOR/DNA COMPLEX'''<br />
 
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==Overview==
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==HEAT SHOCK TRANSCRIPTION FACTOR/DNA COMPLEX==
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The 1.75 A crystal structure of the Kluyveromyces lactis heat shock, transcription factor (HSF) DNA-binding domain (DBD) complexed with DNA, reveals a protein-DNA interface with few direct major groove contacts and, a number of phosphate backbone contacts that are primarily water-mediated, interactions. The DBD, a 'winged' helix-turn-helix protein, displays a, novel mode of binding in that the 'wing' does not contact DNA like all, others of that class. Instead, the monomeric DBD, which crystallized as a, symmetric dimer to a pair of nGAAn inverted repeats, uses the 'wing' to, form part of the protein-protein contacts. This dimer interface is likely, important for increasing the DNA-binding specificity and affinity of the, trimeric form of HSF, as well as for increasing cooperativity between, adjacent trimers.
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<StructureSection load='3hts' size='340' side='right'caption='[[3hts]], [[Resolution|resolution]] 1.75&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3hts]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Kluyveromyces_lactis Kluyveromyces lactis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HTS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3HTS FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.75&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3hts FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3hts OCA], [https://pdbe.org/3hts PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3hts RCSB], [https://www.ebi.ac.uk/pdbsum/3hts PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3hts ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/HSF_KLULA HSF_KLULA] DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. Also required for growth at normal temperatures.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ht/3hts_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3hts ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The 1.75 A crystal structure of the Kluyveromyces lactis heat shock transcription factor (HSF) DNA-binding domain (DBD) complexed with DNA reveals a protein-DNA interface with few direct major groove contacts and a number of phosphate backbone contacts that are primarily water-mediated interactions. The DBD, a 'winged' helix-turn-helix protein, displays a novel mode of binding in that the 'wing' does not contact DNA like all others of that class. Instead, the monomeric DBD, which crystallized as a symmetric dimer to a pair of nGAAn inverted repeats, uses the 'wing' to form part of the protein-protein contacts. This dimer interface is likely important for increasing the DNA-binding specificity and affinity of the trimeric form of HSF, as well as for increasing cooperativity between adjacent trimers.
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==About this Structure==
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A new use for the 'wing' of the 'winged' helix-turn-helix motif in the HSF-DNA cocrystal.,Littlefield O, Nelson HC Nat Struct Biol. 1999 May;6(5):464-70. PMID:10331875<ref>PMID:10331875</ref>
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3HTS is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Kluyveromyces_lactis Kluyveromyces lactis] with GOL as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=3HTS OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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A new use for the 'wing' of the 'winged' helix-turn-helix motif in the HSF-DNA cocrystal., Littlefield O, Nelson HC, Nat Struct Biol. 1999 May;6(5):464-70. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10331875 10331875]
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</div>
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[[Category: Kluyveromyces lactis]]
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<div class="pdbe-citations 3hts" style="background-color:#fffaf0;"></div>
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[[Category: Single protein]]
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[[Category: Littlefield, O.]]
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[[Category: Nelson, H.C.M.]]
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[[Category: GOL]]
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[[Category: complex (winged helix_turn_ helix/dna)]]
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[[Category: dna-binding protein]]
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[[Category: transcription regulation]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 19:47:28 2007''
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==See Also==
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*[[Heat shock factor|Heat shock factor]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Kluyveromyces lactis]]
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[[Category: Large Structures]]
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[[Category: Littlefield O]]
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[[Category: Nelson HCM]]

Current revision

HEAT SHOCK TRANSCRIPTION FACTOR/DNA COMPLEX

PDB ID 3hts

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