1ksk

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
(New page: 200px<br /><applet load="1ksk" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ksk, resolution 2.0&Aring;" /> '''STRUCTURE OF RSUA'''<...)
Current revision (08:35, 6 November 2024) (edit) (undo)
 
(17 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1ksk.gif|left|200px]]<br /><applet load="1ksk" size="450" color="white" frame="true" align="right" spinBox="true"
 
-
caption="1ksk, resolution 2.0&Aring;" />
 
-
'''STRUCTURE OF RSUA'''<br />
 
-
==Overview==
+
==STRUCTURE OF RSUA==
-
In Escherichia coli, the pseudouridine synthase RsuA catalyzes formation, of pseudouridine (psi) at position 516 in 16S rRNA during assembly of the, 30S ribosomal subunit. We have determined the crystal structure of RsuA, bound to uracil at 2.0 A resolution and to uridine 5'-monophosphate (UMP), at 2.65 A resolution. RsuA consists of an N-terminal domain connected by, an extended linker to the central and C-terminal domains. Uracil and UMP, bind in a cleft between the central and C-terminal domains near the, catalytic residue Asp 102. The N-terminal domain shows structural, similarity to the ribosomal protein S4. Despite only 15% amino acid, identity, the other two domains are structurally similar to those of the, tRNA-specific psi-synthase TruA, including the position of the catalytic, Asp. Our results suggest that all four families of pseudouridine synthases, share the same fold of their catalytic domain(s) and uracil-binding site.
+
<StructureSection load='1ksk' size='340' side='right'caption='[[1ksk]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
 +
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[1ksk]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KSK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1KSK FirstGlance]. <br>
 +
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
 +
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=URA:URACIL'>URA</scene></td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ksk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ksk OCA], [https://pdbe.org/1ksk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ksk RCSB], [https://www.ebi.ac.uk/pdbsum/1ksk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ksk ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/RSUA_ECOLI RSUA_ECOLI] Responsible for synthesis of pseudouridine from uracil-516 in 16S ribosomal RNA.<ref>PMID:7612632</ref> <ref>PMID:10376875</ref>
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ks/1ksk_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ksk ConSurf].
 +
<div style="clear:both"></div>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
In Escherichia coli, the pseudouridine synthase RsuA catalyzes formation of pseudouridine (psi) at position 516 in 16S rRNA during assembly of the 30S ribosomal subunit. We have determined the crystal structure of RsuA bound to uracil at 2.0 A resolution and to uridine 5'-monophosphate (UMP) at 2.65 A resolution. RsuA consists of an N-terminal domain connected by an extended linker to the central and C-terminal domains. Uracil and UMP bind in a cleft between the central and C-terminal domains near the catalytic residue Asp 102. The N-terminal domain shows structural similarity to the ribosomal protein S4. Despite only 15% amino acid identity, the other two domains are structurally similar to those of the tRNA-specific psi-synthase TruA, including the position of the catalytic Asp. Our results suggest that all four families of pseudouridine synthases share the same fold of their catalytic domain(s) and uracil-binding site.
-
==About this Structure==
+
Structure of the 16S rRNA pseudouridine synthase RsuA bound to uracil and UMP.,Sivaraman J, Sauve V, Larocque R, Stura EA, Schrag JD, Cygler M, Matte A Nat Struct Biol. 2002 May;9(5):353-8. PMID:11953756<ref>PMID:11953756</ref>
-
1KSK is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with URA as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Pseudouridylate_synthase Pseudouridylate synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.70 4.2.1.70] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1KSK OCA].
+
-
==Reference==
+
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-
Structure of the 16S rRNA pseudouridine synthase RsuA bound to uracil and UMP., Sivaraman J, Sauve V, Larocque R, Stura EA, Schrag JD, Cygler M, Matte A, Nat Struct Biol. 2002 May;9(5):353-8. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11953756 11953756]
+
</div>
-
[[Category: Escherichia coli]]
+
<div class="pdbe-citations 1ksk" style="background-color:#fffaf0;"></div>
-
[[Category: Pseudouridylate synthase]]
+
-
[[Category: Single protein]]
+
-
[[Category: Cygler, M.]]
+
-
[[Category: Larocque, R.]]
+
-
[[Category: Matte, A.]]
+
-
[[Category: Sauve, V.]]
+
-
[[Category: Schrag, J.D.]]
+
-
[[Category: Sivaraman, J.]]
+
-
[[Category: Stura, E.A.]]
+
-
[[Category: URA]]
+
-
[[Category: crystal structure]]
+
-
[[Category: pseudouridine synthase]]
+
-
[[Category: rsua]]
+
-
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 19:47:58 2007''
+
==See Also==
 +
*[[Guide-independent Pseudouridine synthase|Guide-independent Pseudouridine synthase]]
 +
*[[Pseudouridine synthase 3D structures|Pseudouridine synthase 3D structures]]
 +
== References ==
 +
<references/>
 +
__TOC__
 +
</StructureSection>
 +
[[Category: Escherichia coli]]
 +
[[Category: Large Structures]]
 +
[[Category: Cygler M]]
 +
[[Category: Larocque R]]
 +
[[Category: Matte A]]
 +
[[Category: Sauve V]]
 +
[[Category: Schrag JD]]
 +
[[Category: Sivaraman J]]
 +
[[Category: Stura EA]]

Current revision

STRUCTURE OF RSUA

PDB ID 1ksk

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1ksk"
Personal tools