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3nse

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(New page: 200px<br /><applet load="3nse" size="450" color="white" frame="true" align="right" spinBox="true" caption="3nse, resolution 2.1&Aring;" /> '''BOVINE ENOS, H4B-FREE...)
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[[Image:3nse.jpg|left|200px]]<br /><applet load="3nse" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="3nse, resolution 2.1&Aring;" />
 
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'''BOVINE ENOS, H4B-FREE, SEITU COMPLEX'''<br />
 
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==Overview==
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==BOVINE ENOS, H4B-FREE, SEITU COMPLEX==
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Nitric oxide, a key signaling molecule, is produced by a family of enzymes, collectively called nitric oxide synthases (NOS). Here, we report the, crystal structure of the heme domain of endothelial NOS in, tetrahydrobiopterin (H4B)-free and -bound forms at 1.95 A and 1.9 A, resolution, respectively. In both structures a zinc ion is tetrahedrally, coordinated to pairs of symmetry-related cysteine residues at the dimer, interface. The phylogenetically conserved Cys-(X)4-Cys motif and its, strategic location establish a structural role for the metal center in, maintaining the integrity of the H4B-binding site. The unexpected, recognition of the substrate, L-arginine, at the H4B site indicates that, this site is poised to stabilize a positively charged pterin ring and, suggests a model involving a cationic pterin radical in the catalytic, cycle.
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<StructureSection load='3nse' size='340' side='right'caption='[[3nse]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3nse]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NSE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3NSE FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=ARG:ARGININE'>ARG</scene>, <scene name='pdbligand=CAC:CACODYLATE+ION'>CAC</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=ITU:ETHYLISOTHIOUREA'>ITU</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3nse FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3nse OCA], [https://pdbe.org/3nse PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3nse RCSB], [https://www.ebi.ac.uk/pdbsum/3nse PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3nse ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/NOS3_BOVIN NOS3_BOVIN] Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ns/3nse_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3nse ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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3NSE is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] with ACT, CAC, ZN, ARG, HEM, ITU and GOL as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Nitric-oxide_synthase Nitric-oxide synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.13.39 1.14.13.39] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=3NSE OCA].
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*[[Nitric Oxide Synthase 3D structures|Nitric Oxide Synthase 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Crystal structure of constitutive endothelial nitric oxide synthase: a paradigm for pterin function involving a novel metal center., Raman CS, Li H, Martasek P, Kral V, Masters BS, Poulos TL, Cell. 1998 Dec 23;95(7):939-50. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9875848 9875848]
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[[Category: Bos taurus]]
[[Category: Bos taurus]]
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[[Category: Nitric-oxide synthase]]
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[[Category: Large Structures]]
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[[Category: Single protein]]
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[[Category: Kral V]]
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[[Category: Kral, V.]]
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[[Category: Li H]]
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[[Category: Li, H.]]
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[[Category: Martasek P]]
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[[Category: Martasek, P.]]
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[[Category: Masters BSS]]
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[[Category: Masters, B.S.S.]]
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[[Category: Poulos TL]]
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[[Category: Poulos, T.L.]]
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[[Category: Raman CS]]
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[[Category: Raman, C.S.]]
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[[Category: ACT]]
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[[Category: ARG]]
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[[Category: CAC]]
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[[Category: GOL]]
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[[Category: HEM]]
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[[Category: ITU]]
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[[Category: ZN]]
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[[Category: complex (oxidoreductase/peptide)]]
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[[Category: heme protein]]
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[[Category: nitric oxide synthase]]
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[[Category: tetrahydrobiopterin]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 19:53:45 2007''
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Current revision

BOVINE ENOS, H4B-FREE, SEITU COMPLEX

PDB ID 3nse

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