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3pcy

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(New page: 200px<br /><applet load="3pcy" size="450" color="white" frame="true" align="right" spinBox="true" caption="3pcy, resolution 1.9&Aring;" /> '''THE CRYSTAL STRUCTURE...)
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[[Image:3pcy.jpg|left|200px]]<br /><applet load="3pcy" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="3pcy, resolution 1.9&Aring;" />
 
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'''THE CRYSTAL STRUCTURE OF MERCURY-SUBSTITUTED POPLAR PLASTOCYANIN AT 1.9-ANGSTROMS RESOLUTION'''<br />
 
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==Overview==
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==THE CRYSTAL STRUCTURE OF MERCURY-SUBSTITUTED POPLAR PLASTOCYANIN AT 1.9-ANGSTROMS RESOLUTION==
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The crystal structure of Hg(II)-plastocyanin has been determined and, refined at a resolution of 1.9 A. The crystals were prepared by soaking, crystals of Cu(II)-plastocyanin from poplar leaves (Populus nigra var., italica) in a solution of a mercuric salt. Replacement of the Cu(II) atom, in plastocyanin by Hg(II) causes only minor changes in the geometry of the, metal site, and there are few significant changes elsewhere in the, molecule. It is concluded that, as in the case of the native protein, the, geometry of the metal site is determined by the polypeptide. The weak, metal-S(methionine) bond found in Cu(II)-plastocyanin remains weak in, Hg(II)-plastocyanin. The "flip" of a proline side chain close to the metal, site from a C gamma-exo conformation in Cu(II)-plastocyanin to a C, gamma-endo conformation in Hg(II)-plastocyanin suggests that this region, of the molecule is particularly flexible. Crystallographic evidence for, the close similarity of the Hg(II)- and Cu(II)-plastocyanin structures was, originally obtained from electron density difference maps at 2.5-A, resolution. The refinement of the structure was begun with a set of atomic, coordinates taken from the structure of Cu(II)-plastocyanin. A Hg(II) atom, was substituted for the Cu(II) atom, and the side chains of 6 residues in, the vicinity of the metal site were omitted. Three series of, stereochemically restrained least-squares refinement calculations were, interspersed with two stages of model adjustment followed by phase, extension. Fifty-nine water molecules were located. The final structure, has a crystallographic residual R = 0.16.
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<StructureSection load='3pcy' size='340' side='right'caption='[[3pcy]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3pcy]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Populus_nigra Populus nigra]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PCY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3PCY FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HG:MERCURY+(II)+ION'>HG</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3pcy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3pcy OCA], [https://pdbe.org/3pcy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3pcy RCSB], [https://www.ebi.ac.uk/pdbsum/3pcy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3pcy ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/PLAS1_POPNI PLAS1_POPNI] Participates in electron transfer between P700 and the cytochrome b6-f complex in photosystem I.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/pc/3pcy_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3pcy ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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3PCY is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Populus_nigra Populus nigra] with HG as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=3PCY OCA].
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*[[Plastocyanin 3D structures|Plastocyanin 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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The crystal structure of mercury-substituted poplar plastocyanin at 1.9-A resolution., Church WB, Guss JM, Potter JJ, Freeman HC, J Biol Chem. 1986 Jan 5;261(1):234-7. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=3941073 3941073]
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[[Category: Large Structures]]
[[Category: Populus nigra]]
[[Category: Populus nigra]]
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[[Category: Single protein]]
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[[Category: Church WB]]
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[[Category: Church, W.B.]]
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[[Category: Freeman HC]]
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[[Category: Freeman, H.C.]]
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[[Category: Guss JM]]
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[[Category: Guss, J.M.]]
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[[Category: Potter JJ]]
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[[Category: Potter, J.J.]]
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[[Category: HG]]
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[[Category: electron transport protein(cuproprotein)]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 19:55:18 2007''
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Current revision

THE CRYSTAL STRUCTURE OF MERCURY-SUBSTITUTED POPLAR PLASTOCYANIN AT 1.9-ANGSTROMS RESOLUTION

PDB ID 3pcy

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