1sez

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{{Seed}}
 
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[[Image:1sez.png|left|200px]]
 
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==Crystal Structure of Protoporphyrinogen IX Oxidase==
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The line below this paragraph, containing "STRUCTURE_1sez", creates the "Structure Box" on the page.
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<StructureSection load='1sez' size='340' side='right'caption='[[1sez]], [[Resolution|resolution]] 2.90&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1sez]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Nicotiana_tabacum Nicotiana tabacum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SEZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1SEZ FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.9&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=OMN:4-BROMO-3-(5-CARBOXY-4-CHLORO-2-FLUOROPHENYL)-1-METHYL-5-TRIFLUOROMETHYL-PYRAZOL'>OMN</scene>, <scene name='pdbligand=TON:2-{2-[4-(1,1,3,3-TETRAMETHYLBUTYL)PHENOXY]ETHOXY}ETHANOL'>TON</scene></td></tr>
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{{STRUCTURE_1sez| PDB=1sez | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1sez FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1sez OCA], [https://pdbe.org/1sez PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1sez RCSB], [https://www.ebi.ac.uk/pdbsum/1sez PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1sez ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/PPOM_TOBAC PPOM_TOBAC] Catalyzes the 6-electron oxidation of protoporphyrinogen-IX to form protoporphyrin-IX.<ref>PMID:9238074</ref> Provides precursor for the mitochondrial and plastidic heme synthesis and the predominant chlorophyll synthesis in plastids.<ref>PMID:9238074</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/se/1sez_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1sez ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Protoporphyrinogen IX oxidase (PPO), the last common enzyme of haem and chlorophyll biosynthesis, catalyses the oxidation of protoporphyrinogen IX to protoporphyrin IX. The membrane-embedded flavoprotein is the target of a large class of herbicides. In humans, a defect in PPO is responsible for the dominantly inherited disease variegate porphyria. Here we present the crystal structure of mitochondrial PPO from tobacco complexed with a phenyl-pyrazol inhibitor. PPO forms a loosely associated dimer and folds into an FAD-binding domain of the p-hydroxybenzoate-hydrolase fold and a substrate-binding domain that enclose a narrow active site cavity beneath the FAD and an alpha-helical membrane-binding domain. The active site architecture suggests a specific substrate-binding mode compatible with the unusual six-electron oxidation. The membrane-binding domains can be docked onto the dimeric structure of human ferrochelatase, the next enzyme in haem biosynthesis, embedded in the opposite side of the membrane. This modelled transmembrane complex provides a structural explanation for the uncoupling of haem biosynthesis observed in variegate porphyria patients and in plants after inhibiting PPO.
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===Crystal Structure of Protoporphyrinogen IX Oxidase===
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Crystal structure of protoporphyrinogen IX oxidase: a key enzyme in haem and chlorophyll biosynthesis.,Koch M, Breithaupt C, Kiefersauer R, Freigang J, Huber R, Messerschmidt A EMBO J. 2004 Apr 21;23(8):1720-8. Epub 2004 Apr 1. PMID:15057273<ref>PMID:15057273</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_15057273}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 1sez" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 15057273 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_15057273}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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1SEZ is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Nicotiana_tabacum Nicotiana tabacum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SEZ OCA].
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==Reference==
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Crystal structure of protoporphyrinogen IX oxidase: a key enzyme in haem and chlorophyll biosynthesis., Koch M, Breithaupt C, Kiefersauer R, Freigang J, Huber R, Messerschmidt A, EMBO J. 2004 Apr 21;23(8):1720-8. Epub 2004 Apr 1. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15057273 15057273]
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[[Category: Nicotiana tabacum]]
[[Category: Nicotiana tabacum]]
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[[Category: Protoporphyrinogen oxidase]]
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[[Category: Breithaupt C]]
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[[Category: Single protein]]
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[[Category: Freigang J]]
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[[Category: Breithaupt, C.]]
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[[Category: Huber R]]
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[[Category: Freigang, J.]]
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[[Category: Kiefersauer R]]
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[[Category: Huber, R.]]
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[[Category: Koch M]]
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[[Category: Kiefersauer, R.]]
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[[Category: Messerschmidt A]]
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[[Category: Koch, M.]]
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[[Category: Messerschmidt, A.]]
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[[Category: Fad-binding]]
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[[Category: Monotopic membrane-binding domain]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 23:38:12 2008''
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Current revision

Crystal Structure of Protoporphyrinogen IX Oxidase

PDB ID 1sez

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