2bj0

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{{Seed}}
 
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[[Image:2bj0.png|left|200px]]
 
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==Crystal Structure of AChBP from Bulinus truncatus revals the conserved structural scaffold and sites of variation in nicotinic acetylcholine receptors==
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The line below this paragraph, containing "STRUCTURE_2bj0", creates the "Structure Box" on the page.
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<StructureSection load='2bj0' size='340' side='right'caption='[[2bj0]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2bj0]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Bulinus_truncatus Bulinus truncatus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BJ0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2BJ0 FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CXS:3-CYCLOHEXYL-1-PROPYLSULFONIC+ACID'>CXS</scene></td></tr>
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{{STRUCTURE_2bj0| PDB=2bj0 | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2bj0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bj0 OCA], [https://pdbe.org/2bj0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2bj0 RCSB], [https://www.ebi.ac.uk/pdbsum/2bj0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2bj0 ProSAT]</span></td></tr>
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</table>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bj/2bj0_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2bj0 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The crystal structure of acetylcholine-binding protein (AChBP) from the mollusk Lymnaea stagnalis is the established model for the ligand binding domains of the ligand-gated ion channel family, which includes nicotinic acetylcholine, 5-hydroxytryptamine (5-HT3), gamma-aminobutyric acid (GABA), types A and C, and glycine receptors. Here we present the crystal structure of a remote homolog, AChBP from Bulinus truncatus, which reveals both the conserved structural scaffold and the sites of variation in this receptor family. These include rigid body movements of loops that are close to the transmembrane interface in the receptors and changes in the intermonomer contacts, which alter the pentamer stability drastically. Structural, pharmacological and mutational analysis of both AChBPs shows how 3 amino acid changes in the binding site contribute to a 5-10-fold difference in affinity for nicotinic ligands. Comparison of these structures will be valuable for improving structure-function studies of ligand-gated ion channel receptors, including signal transduction, homology modeling, and drug design.
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===CRYSTAL STRUCTURE OF ACHBP FROM BULINUS TRUNCATUS REVALS THE CONSERVED STRUCTURAL SCAFFOLD AND SITES OF VARIATION IN NICOTINIC ACETYLCHOLINE RECEPTORS===
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Crystal structure of acetylcholine-binding protein from Bulinus truncatus reveals the conserved structural scaffold and sites of variation in nicotinic acetylcholine receptors.,Celie PH, Klaassen RV, van Rossum-Fikkert SE, van Elk R, van Nierop P, Smit AB, Sixma TK J Biol Chem. 2005 Jul 15;280(28):26457-66. Epub 2005 May 16. PMID:15899893<ref>PMID:15899893</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 2bj0" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_15899893}}, adds the Publication Abstract to the page
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*[[Acetylcholine binding protein 3D structures|Acetylcholine binding protein 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 15899893 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_15899893}}
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__TOC__
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</StructureSection>
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==About this Structure==
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2BJ0 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bulinus_truncatus Bulinus truncatus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BJ0 OCA].
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==Reference==
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Crystal structure of acetylcholine-binding protein from Bulinus truncatus reveals the conserved structural scaffold and sites of variation in nicotinic acetylcholine receptors., Celie PH, Klaassen RV, van Rossum-Fikkert SE, van Elk R, van Nierop P, Smit AB, Sixma TK, J Biol Chem. 2005 Jul 15;280(28):26457-66. Epub 2005 May 16. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15899893 15899893]
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[[Category: Bulinus truncatus]]
[[Category: Bulinus truncatus]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Celie, P H.N.]]
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[[Category: Celie PHN]]
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[[Category: Elk, R Van.]]
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[[Category: Klaassen RV]]
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[[Category: Klaassen, R V.]]
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[[Category: Sixma TK]]
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[[Category: Nierop, P Van.]]
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[[Category: Smit AB]]
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[[Category: Rossum-Fikkert, S E.Van.]]
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[[Category: Van Elk R]]
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[[Category: Sixma, T K.]]
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[[Category: Van Nierop P]]
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[[Category: Smit, A B.]]
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[[Category: Van Rossum-Fikkert SE]]
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[[Category: 3d-structure]]
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[[Category: Glycoprotein]]
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[[Category: Glycprotein]]
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[[Category: Igg fold]]
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[[Category: Immunoglobulin domain]]
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[[Category: Pentamer]]
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[[Category: Signal]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 23:45:12 2008''
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Current revision

Crystal Structure of AChBP from Bulinus truncatus revals the conserved structural scaffold and sites of variation in nicotinic acetylcholine receptors

PDB ID 2bj0

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