1us6

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{{Seed}}
 
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[[Image:1us6.png|left|200px]]
 
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==Crystal structure of the quorum-sensing protein TraM from Agrobacterium tumefaciens at 1.65 Ang. resolution==
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The line below this paragraph, containing "STRUCTURE_1us6", creates the "Structure Box" on the page.
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<StructureSection load='1us6' size='340' side='right'caption='[[1us6]], [[Resolution|resolution]] 1.65&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1us6]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Agrobacterium_tumefaciens Agrobacterium tumefaciens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1US6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1US6 FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.65&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1us6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1us6 OCA], [https://pdbe.org/1us6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1us6 RCSB], [https://www.ebi.ac.uk/pdbsum/1us6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1us6 ProSAT]</span></td></tr>
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{{STRUCTURE_1us6| PDB=1us6 | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/TRAM_RHIRD TRAM_RHIRD] Negatively regulates conjugation and the expression of tra genes by antagonizing TraR/AAI-dependent activation. TraM may either bind or modify TraR or AAI making them unavailable. Alternatively, TraM may bind tra promoters preventing TraR activation.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/us/1us6_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1us6 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Transfer of the tumor-inducing plasmid in Agrobacterium tumefaciens is controlled by a quorum-sensing system whose main components are the transcriptional regulator TraR and its autoinducer. This system allows bacteria to synchronize infection of the host plant when a "quorum" of cells has been reached. TraM is an A. tumefaciens protein involved in the regulation of this system because it binds to TraR and prevents it from binding DNA. As a first step to understanding the molecular basis for the regulation of TraR by TraM, we have determined the crystal structure of TraM at 1.65 A resolution. This protein is packed as a dimer, with each monomer consisting mainly of two antiparallel alpha helices. Monomers are tightly associated, with a large hydrophobic area buried upon dimerization. Secondly, we characterized the TraR-TraM complex in vitro. TraM (11.4 kDa, monomer molecular mass) binds tightly TraR (27 kDa, monomer molecular mass) forming a stable oligomeric complex that likely accounts for two TraR and two TraM dimers.
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===CRYSTAL STRUCTURE OF THE QUORUM-SENSING PROTEIN TRAM FROM AGROBACTERIUM TUMEFACIENS AT 1.65 ANG. RESOLUTION===
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Crystal structure of the quorum-sensing protein TraM and its interaction with the transcriptional regulator TraR.,Vannini A, Volpari C, Di Marco S J Biol Chem. 2004 Jun 4;279(23):24291-6. Epub 2004 Mar 24. PMID:15044488<ref>PMID:15044488</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_15044488}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 1us6" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 15044488 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_15044488}}
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__TOC__
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</StructureSection>
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==About this Structure==
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1US6 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Agrobacterium_tumefaciens Agrobacterium tumefaciens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1US6 OCA].
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==Reference==
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Crystal structure of the quorum-sensing protein TraM and its interaction with the transcriptional regulator TraR., Vannini A, Volpari C, Di Marco S, J Biol Chem. 2004 Jun 4;279(23):24291-6. Epub 2004 Mar 24. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15044488 15044488]
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[[Category: Agrobacterium tumefaciens]]
[[Category: Agrobacterium tumefaciens]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Marco, S Di.]]
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[[Category: Di Marco S]]
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[[Category: Vannini, A.]]
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[[Category: Vannini A]]
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[[Category: Conjugation]]
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[[Category: Negative regulator]]
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[[Category: Plasmid.]]
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[[Category: Quorum-sensing]]
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[[Category: Repressor]]
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[[Category: Transcripti regulation]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 23:58:29 2008''
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Current revision

Crystal structure of the quorum-sensing protein TraM from Agrobacterium tumefaciens at 1.65 Ang. resolution

PDB ID 1us6

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