3blw

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{{Seed}}
 
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[[Image:3blw.png|left|200px]]
 
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==Yeast Isocitrate Dehydrogenase with Citrate and AMP Bound in the Regulatory Subunits==
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The line below this paragraph, containing "STRUCTURE_3blw", creates the "Structure Box" on the page.
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<StructureSection load='3blw' size='340' side='right'caption='[[3blw]], [[Resolution|resolution]] 4.30&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3blw]] is a 16 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. The September 2010 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Isocitrate Dehydrogenase'' by David Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2010_9 10.2210/rcsb_pdb/mom_2010_9]. The October 2012 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Citric Acid Cycle'' by David Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2012_10 10.2210/rcsb_pdb/mom_2012_10]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BLW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3BLW FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 4.3&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AMP:ADENOSINE+MONOPHOSPHATE'>AMP</scene>, <scene name='pdbligand=FLC:CITRATE+ANION'>FLC</scene></td></tr>
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{{STRUCTURE_3blw| PDB=3blw | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3blw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3blw OCA], [https://pdbe.org/3blw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3blw RCSB], [https://www.ebi.ac.uk/pdbsum/3blw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3blw ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/IDH1_YEAST IDH1_YEAST] Performs an essential role in the oxidative function of the citric acid cycle. Also binds RNA; specifically to the 5'-untranslated leaders of mitochondrial mRNAs.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bl/3blw_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3blw ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Mitochondrial NAD(+)-specific isocitrate dehydrogenases (IDHs) are key regulators of flux through biosynthetic and oxidative pathways in response to cellular energy levels. Here we present the first structures of a eukaryotic member of this enzyme family, the allosteric, hetero-octameric, NAD(+)-specific IDH from yeast in three forms: 1) without ligands, 2) with bound analog citrate, and 3) with bound citrate + AMP. The structures reveal the molecular basis for ligand binding to homologous but distinct regulatory and catalytic sites positioned at the interfaces between IDH1 and IDH2 subunits and define pathways of communication between heterodimers and heterotetramers in the hetero-octamer. Disulfide bonds observed at the heterotetrameric interfaces in the unliganded IDH hetero-octamer are reduced in the ligand-bound forms, suggesting a redox regulatory mechanism that may be analogous to the "on-off" regulation of non-allosteric bacterial IDHs via phosphorylation. The results strongly suggest that eukaryotic IDH enzymes are exquisitely tuned to ensure that allosteric activation occurs only when concentrations of isocitrate are elevated.
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===Yeast Isocitrate Dehydrogenase with Citrate and AMP Bound in the Regulatory Subunits===
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Allosteric motions in structures of yeast NAD+-specific isocitrate dehydrogenase.,Taylor AB, Hu G, Hart PJ, McAlister-Henn L J Biol Chem. 2008 Apr 18;283(16):10872-80. Epub 2008 Feb 6. PMID:18256028<ref>PMID:18256028</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3blw" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_18256028}}, adds the Publication Abstract to the page
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*[[Isocitrate dehydrogenase 3D structures|Isocitrate dehydrogenase 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 18256028 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_18256028}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Citric Acid Cycle]]
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3BLW is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BLW OCA].
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[[Category: Isocitrate Dehydrogenase]]
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[[Category: Large Structures]]
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==Reference==
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[[Category: RCSB PDB Molecule of the Month]]
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Allosteric motions in structures of yeast NAD+-specific isocitrate dehydrogenase., Taylor AB, Hu G, Hart PJ, McAlister-Henn L, J Biol Chem. 2008 Apr 18;283(16):10872-80. Epub 2008 Feb 6. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18256028 18256028]
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[[Category: Protein complex]]
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[[Category: Saccharomyces cerevisiae]]
[[Category: Saccharomyces cerevisiae]]
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[[Category: Hart, P J.]]
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[[Category: Hart PJ]]
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[[Category: Hu, G.]]
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[[Category: Hu G]]
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[[Category: McAlister-Henn, L.]]
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[[Category: McAlister-Henn L]]
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[[Category: Taylor, A B.]]
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[[Category: Taylor AB]]
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[[Category: Allosteric enzyme]]
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[[Category: Allostery]]
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[[Category: Decarboxylase]]
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[[Category: Dehydrogenase]]
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[[Category: Magnesium]]
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[[Category: Manganese]]
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[[Category: Metal-binding]]
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[[Category: Mitochondrion]]
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[[Category: Nad]]
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[[Category: Oxidative metabolism]]
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[[Category: Oxidoreductase]]
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[[Category: Phosphoprotein]]
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[[Category: Rna-binding]]
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[[Category: Tca cycle]]
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[[Category: Transit peptide]]
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[[Category: Tricarboxylic acid cycle]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 00:02:50 2008''
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Current revision

Yeast Isocitrate Dehydrogenase with Citrate and AMP Bound in the Regulatory Subunits

PDB ID 3blw

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