2q0q

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{{Seed}}
 
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[[Image:2q0q.png|left|200px]]
 
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==Structure of the Native M. Smegmatis Aryl Esterase==
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The line below this paragraph, containing "STRUCTURE_2q0q", creates the "Structure Box" on the page.
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<StructureSection load='2q0q' size='340' side='right'caption='[[2q0q]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2q0q]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycolicibacterium_smegmatis Mycolicibacterium smegmatis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Q0Q OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2Q0Q FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.5&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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{{STRUCTURE_2q0q| PDB=2q0q | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2q0q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2q0q OCA], [https://pdbe.org/2q0q PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2q0q RCSB], [https://www.ebi.ac.uk/pdbsum/2q0q PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2q0q ProSAT]</span></td></tr>
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</table>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/q0/2q0q_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2q0q ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The unusual architecture of the enzyme (MsAcT) isolated from Mycobacterium smegmatis forms the mechanistic basis for favoring alcoholysis over hydrolysis in water. Unlike hydrolases that perform alcoholysis only under anhydrous conditions, MsAcT demonstrates alcoholysis in substantially aqueous media and, in the presence of hydrogen peroxide, has a perhydrolysis:hydrolysis ratio 50-fold greater than that of the best lipase tested. The crystal structures of the apoenzyme and an inhibitor-bound form have been determined to 1.5 A resolution. MsAcT is an octamer in the asymmetric unit and forms a tightly associated aggregate in solution. Relative to other structurally similar monomers, MsAcT contains several insertions that contribute to the oligomerization and greatly restrict the shape of the active site, thereby limiting its accessibility. These properties create an environment by which MsAcT can catalyze transesterification reactions in an aqueous medium and suggests how a serine hydrolase can be engineered to be an efficient acyltransferase.
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===Structure of the Native M. Smegmatis Aryl Esterase===
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Structure of a novel enzyme that catalyzes acyl transfer to alcohols in aqueous conditions.,Mathews I, Soltis M, Saldajeno M, Ganshaw G, Sala R, Weyler W, Cervin MA, Whited G, Bott R Biochemistry. 2007 Aug 7;46(31):8969-79. Epub 2007 Jul 18. PMID:17636869<ref>PMID:17636869</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_17636869}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 2q0q" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 17636869 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_17636869}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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2Q0Q is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Mycobacterium_smegmatis Mycobacterium smegmatis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Q0Q OCA].
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[[Category: Mycolicibacterium smegmatis]]
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[[Category: Bott R]]
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==Reference==
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[[Category: Cervin MA]]
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Structure of a novel enzyme that catalyzes acyl transfer to alcohols in aqueous conditions., Mathews I, Soltis M, Saldajeno M, Ganshaw G, Sala R, Weyler W, Cervin MA, Whited G, Bott R, Biochemistry. 2007 Aug 7;46(31):8969-79. Epub 2007 Jul 18. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17636869 17636869]
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[[Category: Ganshaw G]]
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[[Category: Arylesterase]]
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[[Category: Mathews II]]
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[[Category: Mycobacterium smegmatis]]
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[[Category: Sala R]]
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[[Category: Single protein]]
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[[Category: Saldajeno M]]
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[[Category: Bott, R.]]
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[[Category: Soltis M]]
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[[Category: Cervin, M A.]]
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[[Category: Weyler W]]
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[[Category: Ganshaw, G.]]
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[[Category: Whited G]]
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[[Category: Mathews, I I.]]
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[[Category: Sala, R.]]
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[[Category: Saldajeno, M.]]
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[[Category: Soltis, M.]]
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[[Category: Weyler, W.]]
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[[Category: Whited, G.]]
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[[Category: Acyl transfer]]
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[[Category: Aryl esterase]]
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[[Category: Oligomeric enzyme]]
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[[Category: Sgnh hydrolase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 00:59:59 2008''
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Current revision

Structure of the Native M. Smegmatis Aryl Esterase

PDB ID 2q0q

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