1l0h

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(New page: 200px<br /><applet load="1l0h" size="450" color="white" frame="true" align="right" spinBox="true" caption="1l0h, resolution 2.0&Aring;" /> '''CRYSTAL STRUCTURE OF ...)
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[[Image:1l0h.jpg|left|200px]]<br /><applet load="1l0h" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1l0h, resolution 2.0&Aring;" />
 
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'''CRYSTAL STRUCTURE OF BUTYRYL-ACP FROM E.COLI'''<br />
 
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==Overview==
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==CRYSTAL STRUCTURE OF BUTYRYL-ACP FROM E.COLI==
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Acyl carrier protein (ACP) is an essential cofactor in biosynthesis of, fatty acids and many other reactions that require acyl transfer steps. We, have determined the first crystal structures of an acylated form of ACP, from E. coli, that of butyryl-ACP. Our analysis of the molecular surface, of ACP reveals a plastic hydrophobic cavity in the vicinity of the, phosphopantethylated Ser36 residue that is expanded and occupied by the, butyryl and beta-mercaptoethylamine moieties of the acylated, 4'-phosphopantetheine group in one of our crystal forms. In the other, form, the cavity is contracted, and we propose that the protein has, adopted the conformation after delivery of substrate into the active site, of a partner enzyme.
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<StructureSection load='1l0h' size='340' side='right'caption='[[1l0h]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1l0h]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1L0H OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1L0H FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1l0h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1l0h OCA], [https://pdbe.org/1l0h PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1l0h RCSB], [https://www.ebi.ac.uk/pdbsum/1l0h PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1l0h ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/ACP_ECOLI ACP_ECOLI] Carrier of the growing fatty acid chain in fatty acid biosynthesis.[HAMAP-Rule:MF_01217]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/l0/1l0h_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1l0h ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Acyl carrier protein (ACP) is an essential cofactor in biosynthesis of fatty acids and many other reactions that require acyl transfer steps. We have determined the first crystal structures of an acylated form of ACP from E. coli, that of butyryl-ACP. Our analysis of the molecular surface of ACP reveals a plastic hydrophobic cavity in the vicinity of the phosphopantethylated Ser36 residue that is expanded and occupied by the butyryl and beta-mercaptoethylamine moieties of the acylated 4'-phosphopantetheine group in one of our crystal forms. In the other form, the cavity is contracted, and we propose that the protein has adopted the conformation after delivery of substrate into the active site of a partner enzyme.
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==About this Structure==
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X-ray crystallographic studies on butyryl-ACP reveal flexibility of the structure around a putative acyl chain binding site.,Roujeinikova A, Baldock C, Simon WJ, Gilroy J, Baker PJ, Stuitje AR, Rice DW, Slabas AR, Rafferty JB Structure. 2002 Jun;10(6):825-35. PMID:12057197<ref>PMID:12057197</ref>
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1L0H is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with ZN as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1L0H OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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X-ray crystallographic studies on butyryl-ACP reveal flexibility of the structure around a putative acyl chain binding site., Roujeinikova A, Baldock C, Simon WJ, Gilroy J, Baker PJ, Stuitje AR, Rice DW, Slabas AR, Rafferty JB, Structure. 2002 Jun;10(6):825-35. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12057197 12057197]
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</div>
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[[Category: Escherichia coli]]
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<div class="pdbe-citations 1l0h" style="background-color:#fffaf0;"></div>
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[[Category: Single protein]]
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[[Category: Baker, P.J.]]
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[[Category: Baldock, C.]]
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[[Category: Gilroy, J.]]
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[[Category: Rafferty, J.B.]]
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[[Category: Rice, D.W.]]
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[[Category: Roujeinikova, A.]]
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[[Category: Simon, W.J.]]
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[[Category: Slabas, A.R.]]
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[[Category: Stuitje, A.R.]]
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[[Category: ZN]]
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[[Category: acyl carrier protein]]
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[[Category: acyl chain binding]]
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[[Category: crystal structure]]
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[[Category: fatty acid biosynthesis]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 20:08:04 2007''
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==See Also==
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*[[Acyl carrier protein 3D structures|Acyl carrier protein 3D structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Escherichia coli]]
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[[Category: Large Structures]]
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[[Category: Baker PJ]]
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[[Category: Baldock C]]
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[[Category: Gilroy J]]
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[[Category: Rafferty JB]]
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[[Category: Rice DW]]
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[[Category: Roujeinikova A]]
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[[Category: Simon WJ]]
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[[Category: Slabas AR]]
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[[Category: Stuitje AR]]

Current revision

CRYSTAL STRUCTURE OF BUTYRYL-ACP FROM E.COLI

PDB ID 1l0h

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