1l0o

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(New page: 200px<br /><applet load="1l0o" size="450" color="white" frame="true" align="right" spinBox="true" caption="1l0o, resolution 2.9&Aring;" /> '''Crystal Structure of ...)
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[[Image:1l0o.gif|left|200px]]<br /><applet load="1l0o" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1l0o, resolution 2.9&Aring;" />
 
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'''Crystal Structure of the Bacillus stearothermophilus Anti-Sigma Factor SpoIIAB with the Sporulation Sigma Factor SigmaF'''<br />
 
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==Overview==
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==Crystal Structure of the Bacillus stearothermophilus Anti-Sigma Factor SpoIIAB with the Sporulation Sigma Factor SigmaF==
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Cell type-specific transcription during Bacillus sporulation is, established by sigmaF. SpoIIAB is an anti-sigma that binds and negatively, regulates sigmaF, as well as a serine kinase that phosphorylates and, inactivates the anti-anti-sigma SpoIIAA. The crystal structure of sigmaF, bound to the SpoIIAB dimer in the low-affinity, ADP form has been, determined at 2.9 A resolution. SpoIIAB adopts the GHKL superfamily fold, of ATPases and histidine kinases. A domain of sigmaF contacts both SpoIIAB, monomers, while 80% of the sigma factor is disordered. The interaction, occludes an RNA polymerase binding surface of sigmaF, explaining the, SpoIIAB anti-sigma activity. The structure also explains the specificity, of SpoIIAB for its target sigma factors and, in combination with genetic, and biochemical data, provides insight into the mechanism of SpoIIAA, anti-anti-sigma activity.
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<StructureSection load='1l0o' size='340' side='right'caption='[[1l0o]], [[Resolution|resolution]] 2.90&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1l0o]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Geobacillus_stearothermophilus Geobacillus stearothermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1L0O OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1L0O FirstGlance]. <br>
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1L0O is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Geobacillus_stearothermophilus Geobacillus stearothermophilus] with MG and ADP as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1L0O OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.9&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1l0o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1l0o OCA], [https://pdbe.org/1l0o PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1l0o RCSB], [https://www.ebi.ac.uk/pdbsum/1l0o PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1l0o ProSAT]</span></td></tr>
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Crystal structure of the Bacillus stearothermophilus anti-sigma factor SpoIIAB with the sporulation sigma factor sigmaF., Campbell EA, Masuda S, Sun JL, Muzzin O, Olson CA, Wang S, Darst SA, Cell. 2002 Mar 22;108(6):795-807. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11955433 11955433]
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/SP2AB_GEOSE SP2AB_GEOSE] Binds to sigma F and blocks its ability to form an RNA polymerase holoenzyme (E-sigma F). Phosphorylates SpoIIAA on a serine residue. This phosphorylation may enable SpoIIAA to act as an anti-anti-sigma factor that counteracts SpoIIAB and thus releases sigma F from inhibition.[HAMAP-Rule:MF_00637]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/l0/1l0o_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1l0o ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
[[Category: Geobacillus stearothermophilus]]
[[Category: Geobacillus stearothermophilus]]
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[[Category: Protein complex]]
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[[Category: Large Structures]]
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[[Category: Campbell, E.A.]]
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[[Category: Campbell EA]]
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[[Category: Darst, S.A.]]
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[[Category: Darst SA]]
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[[Category: Masuda, S.]]
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[[Category: Masuda S]]
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[[Category: Muzzin, O.]]
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[[Category: Muzzin O]]
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[[Category: Olson, C.A.]]
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[[Category: Olson CA]]
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[[Category: Sun, J.L.]]
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[[Category: Sun JL]]
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[[Category: Wang, S.]]
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[[Category: Wang S]]
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[[Category: ADP]]
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[[Category: MG]]
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[[Category: bergerat fold]]
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[[Category: helix-turn-helix]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 20:08:36 2007''
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Current revision

Crystal Structure of the Bacillus stearothermophilus Anti-Sigma Factor SpoIIAB with the Sporulation Sigma Factor SigmaF

PDB ID 1l0o

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