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2r29

From Proteopedia

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{{Seed}}
 
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[[Image:2r29.png|left|200px]]
 
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==Neutralization of dengue virus by a serotype cross-reactive antibody elucidated by cryoelectron microscopy and x-ray crystallography==
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The line below this paragraph, containing "STRUCTURE_2r29", creates the "Structure Box" on the page.
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<StructureSection load='2r29' size='340' side='right'caption='[[2r29]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2r29]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Den26 Den26] and [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2R29 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2R29 FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">E protein ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=31634 DEN26])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2r29 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2r29 OCA], [https://pdbe.org/2r29 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2r29 RCSB], [https://www.ebi.ac.uk/pdbsum/2r29 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2r29 ProSAT]</span></td></tr>
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{{STRUCTURE_2r29| PDB=2r29 | SCENE= }}
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</table>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/r2/2r29_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2r29 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The monoclonal antibody 1A1D-2 has been shown to strongly neutralize dengue virus serotypes 1, 2 and 3, primarily by inhibiting attachment to host cells. A crystal structure of its antigen binding fragment (Fab) complexed with domain III of the viral envelope glycoprotein, E, showed that the epitope would be partially occluded in the known structure of the mature dengue virus. Nevertheless, antibody could bind to the virus at 37 degrees C, suggesting that the virus is in dynamic motion making hidden epitopes briefly available. A cryo-electron microscope image reconstruction of the virus:Fab complex showed large changes in the organization of the E protein that exposed the epitopes on two of the three E molecules in each of the 60 icosahedral asymmetric units of the virus. The changes in the structure of the viral surface are presumably responsible for inhibiting attachment to cells.
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===Neutralization of dengue virus by a serotype cross-reactive antibody elucidated by cryoelectron microscopy and x-ray crystallography===
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Binding of a neutralizing antibody to dengue virus alters the arrangement of surface glycoproteins.,Lok SM, Kostyuchenko V, Nybakken GE, Holdaway HA, Battisti AJ, Sukupolvi-Petty S, Sedlak D, Fremont DH, Chipman PR, Roehrig JT, Diamond MS, Kuhn RJ, Rossmann MG Nat Struct Mol Biol. 2008 Mar;15(3):312-7. Epub 2008 Feb 10. PMID:18264114<ref>PMID:18264114</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 2r29" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_18264114}}, adds the Publication Abstract to the page
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*[[Monoclonal Antibodies 3D structures|Monoclonal Antibodies 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 18264114 is the PubMed ID number.
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== References ==
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-->
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<references/>
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{{ABSTRACT_PUBMED_18264114}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Den26]]
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2R29 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Dengue_virus_2 Dengue virus 2] and [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2R29 OCA].
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[[Category: Large Structures]]
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==Reference==
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Binding of a neutralizing antibody to dengue virus alters the arrangement of surface glycoproteins., Lok SM, Kostyuchenko V, Nybakken GE, Holdaway HA, Battisti AJ, Sukupolvi-Petty S, Sedlak D, Fremont DH, Chipman PR, Roehrig JT, Diamond MS, Kuhn RJ, Rossmann MG, Nat Struct Mol Biol. 2008 Mar;15(3):312-7. Epub 2008 Feb 10. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18264114 18264114]
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[[Category: Dengue virus 2]]
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[[Category: Mus musculus]]
[[Category: Mus musculus]]
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[[Category: Protein complex]]
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[[Category: Battisti, A J]]
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[[Category: Battisti, A J.]]
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[[Category: Chipman, P R]]
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[[Category: Chipman, P R.]]
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[[Category: Diamond, M S]]
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[[Category: Diamond, M S.]]
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[[Category: Fremont, D H]]
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[[Category: Fremont, D H.]]
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[[Category: Holdaway, H A]]
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[[Category: Holdaway,H A]]
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[[Category: Kostyuchenko, V K]]
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[[Category: Kostyuchenko, V K.]]
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[[Category: Kuhn, R J]]
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[[Category: Kuhn, R J.]]
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[[Category: Lok, S M]]
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[[Category: Lok, S M.]]
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[[Category: Nybakken, G E]]
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[[Category: Nybakken, G E.]]
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[[Category: Roehrig, J T]]
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[[Category: Roehrig, J T.]]
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[[Category: Rossmann, M G]]
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[[Category: Rossmann, M G.]]
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[[Category: Sedlak, D]]
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[[Category: Sedlak,D.]]
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[[Category: Sukupolvi-petty, S]]
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[[Category: Sukupolvi-petty,S.]]
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[[Category: Atp-binding]]
[[Category: Atp-binding]]
[[Category: Capsid protein]]
[[Category: Capsid protein]]
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[[Category: Transmembrane]]
[[Category: Transmembrane]]
[[Category: Viral nucleoprotein]]
[[Category: Viral nucleoprotein]]
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[[Category: Viral protein/immune system complex]]
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[[Category: Viral protein-immune system complex]]
[[Category: Virion]]
[[Category: Virion]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 01:48:49 2008''
 

Current revision

Neutralization of dengue virus by a serotype cross-reactive antibody elucidated by cryoelectron microscopy and x-ray crystallography

PDB ID 2r29

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