2peh

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{{Seed}}
 
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[[Image:2peh.png|left|200px]]
 
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==Crystal structure of the UHM domain of human SPF45 in complex with SF3b155-ULM5==
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The line below this paragraph, containing "STRUCTURE_2peh", creates the "Structure Box" on the page.
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<StructureSection load='2peh' size='340' side='right'caption='[[2peh]], [[Resolution|resolution]] 2.11&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2peh]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PEH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2PEH FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.11&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2peh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2peh OCA], [https://pdbe.org/2peh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2peh RCSB], [https://www.ebi.ac.uk/pdbsum/2peh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2peh ProSAT]</span></td></tr>
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{{STRUCTURE_2peh| PDB=2peh | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/SPF45_HUMAN SPF45_HUMAN] Splice factor that binds to the single stranded 3'AG at the exon/intron border and promotes its utilization in the second catalytic step. Involved in the regulation of alternative splicing and the utilization of cryptic splice sites. Promotes the utilization of a cryptic splice site created by the beta-110 mutation in the HBB gene. The resulting frameshift leads to sickle cell anemia.<ref>PMID:12015979</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/pe/2peh_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2peh ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The U2AF-homology motif (UHM) mediates protein-protein interactions between factors involved in constitutive RNA splicing. Here we report that the splicing factor SPF45 regulates alternative splicing of the apoptosis regulatory gene FAS (also called CD95). The SPF45 UHM is necessary for this activity and binds UHM-ligand motifs (ULMs) present in the 3' splice site-recognizing factors U2AF65, SF1 and SF3b155. We describe a 2.1-A crystal structure of SPF45-UHM in complex with a ULM peptide from SF3b155. Features distinct from those of previously described UHM-ULM structures allowed the design of mutations in the SPF45 UHM that selectively impair binding to individual ULMs. Splicing assays using the ULM-selective SPF45 variants demonstrate that individual UHM-ULM interactions are required for FAS splicing regulation by SPF45 in vivo. Our data suggest that networks of UHM-ULM interactions are involved in regulating alternative splicing.
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===Crystal structure of the UHM domain of human SPF45 in complex with SF3b155-ULM5===
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U2AF-homology motif interactions are required for alternative splicing regulation by SPF45.,Corsini L, Bonnal S, Basquin J, Hothorn M, Scheffzek K, Valcarcel J, Sattler M Nat Struct Mol Biol. 2007 Jul;14(7):620-9. Epub 2007 Jun 24. PMID:17589525<ref>PMID:17589525</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_17589525}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 2peh" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 17589525 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_17589525}}
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__TOC__
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</StructureSection>
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==About this Structure==
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2PEH is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PEH OCA].
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==Reference==
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U2AF-homology motif interactions are required for alternative splicing regulation by SPF45., Corsini L, Bonnal S, Basquin J, Hothorn M, Scheffzek K, Valcarcel J, Sattler M, Nat Struct Mol Biol. 2007 Jul;14(7):620-9. Epub 2007 Jun 24. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17589525 17589525]
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[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Protein complex]]
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[[Category: Large Structures]]
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[[Category: Basquin, J.]]
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[[Category: Basquin J]]
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[[Category: Corsini, L.]]
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[[Category: Corsini L]]
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[[Category: Hothorn, M.]]
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[[Category: Hothorn M]]
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[[Category: Sattler, M.]]
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[[Category: Sattler M]]
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[[Category: Alternative splicing]]
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[[Category: Rrm]]
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[[Category: Uhm]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 02:11:59 2008''
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Current revision

Crystal structure of the UHM domain of human SPF45 in complex with SF3b155-ULM5

PDB ID 2peh

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