1jwe

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{{Seed}}
 
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[[Image:1jwe.png|left|200px]]
 
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==NMR Structure of the N-Terminal Domain of E. Coli Dnab Helicase==
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The line below this paragraph, containing "STRUCTURE_1jwe", creates the "Structure Box" on the page.
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<StructureSection load='1jwe' size='340' side='right'caption='[[1jwe]]' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1jwe]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JWE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1JWE FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1jwe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jwe OCA], [https://pdbe.org/1jwe PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1jwe RCSB], [https://www.ebi.ac.uk/pdbsum/1jwe PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1jwe ProSAT]</span></td></tr>
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{{STRUCTURE_1jwe| PDB=1jwe | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/DNAB_ECOLI DNAB_ECOLI] Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity and contains distinct active sites for ATP binding, DNA binding, and interaction with DnaC protein, primase, and other prepriming proteins.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/jw/1jwe_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1jwe ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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BACKGROUND: DnaB is the primary replicative helicase in Escherichia coli. Native DnaB is a hexamer of identical subunits, each consisting of a larger C-terminal domain and a smaller N-terminal domain. Electron-microscopy data show hexamers with C6 or C3 symmetry, indicating large domain movements and reversible pairwise association. RESULTS: The three-dimensional structure of the N-terminal domain of E. coli DnaB was determined by nuclear magnetic resonance (NMR) spectroscopy. Structural similarity was found with the primary dimerisation domain of a topoisomerase, the gyrase A subunit from E. coli. A monomer-dimer equilibrium was observed for the isolated N-terminal domain of DnaB. A dimer model with C2 symmetry was derived from intermolecular nuclear Overhauser effects, which is consistent with all available NMR data. CONCLUSIONS: The monomer-dimer equilibrium observed for the N-terminal domain of DnaB is likely to be of functional significance for helicase activity, by participating in the switch between C6 and C3 symmetry of the helicase hexamer.
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===NMR Structure of the N-Terminal Domain of E. Coli Dnab Helicase===
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NMR structure of the N-terminal domain of E. coli DnaB helicase: implications for structure rearrangements in the helicase hexamer.,Weigelt J, Brown SE, Miles CS, Dixon NE, Otting G Structure. 1999 Jun 15;7(6):681-90. PMID:10404597<ref>PMID:10404597</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 1jwe" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_10404597}}, adds the Publication Abstract to the page
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*[[Helicase 3D structures|Helicase 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 10404597 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_10404597}}
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__TOC__
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</StructureSection>
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==About this Structure==
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1JWE is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JWE OCA].
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==Reference==
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NMR structure of the N-terminal domain of E. coli DnaB helicase: implications for structure rearrangements in the helicase hexamer., Weigelt J, Brown SE, Miles CS, Dixon NE, Otting G, Structure. 1999 Jun 15;7(6):681-90. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10404597 10404597]
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[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Brown, S E.]]
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[[Category: Brown SE]]
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[[Category: Dixon, N E.]]
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[[Category: Dixon NE]]
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[[Category: Miles, C S.]]
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[[Category: Miles CS]]
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[[Category: Otting, G.]]
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[[Category: Otting G]]
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[[Category: Weigelt, J.]]
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[[Category: Weigelt J]]
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[[Category: Helicase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 02:23:35 2008''
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Current revision

NMR Structure of the N-Terminal Domain of E. Coli Dnab Helicase

PDB ID 1jwe

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